Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A13 F69 I0 R1 1426 148.0 5875178 97.4% 5722423 123.7

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,883,027 C→G 26.1% intergenic (+30/‑161) dmlA → / → yeaV D‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase/putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,883,0270CG26.1% 26.2 / 7.5 16intergenic (+30/‑161)dmlA/yeaVD‑malate oxidase, NAD‑dependent; putative tartrate dehydrogenase/putative transporter
Reads supporting (aligned to +/- strand):  ref base C (5/6);  new base G (2/2);  total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAT  >  NC_000913/1882885‑1883138
                                                                                                                                              |                                                                                                               
ttCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgc                                                                                                           >  2:711522/1‑149 (MQ=255)
  cTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCgg                                                                                                         <  1:711522/149‑1 (MQ=255)
          aaTTGAAGAAGTGATTGCTCCCGGGCCGAAAACAACTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGGAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAATGACGCGGTTTTTTTa                                                                                                 <  4:1390555/149‑1 (MQ=255)
                    gTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCttt                                                                                       <  2:114232/149‑1 (MQ=255)
                        ttGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgc                                                                                                           <  1:1374413/125‑1 (MQ=255)
                        ttGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGAcgc                                                                                                           >  2:1374413/1‑125 (MQ=255)
                            tCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCa                                                                                    >  3:541494/1‑144 (MQ=255)
                            tCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCa                                                                                    <  4:541494/144‑1 (MQ=255)
                                      aaaaCACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCgg                                                                                                         <  1:1311050/113‑1 (MQ=255)
                                      aaaaCACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCAGTGACGCgg                                                                                                         >  2:1311050/1‑113 (MQ=255)
                                                                                                                                    aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt  >  1:397275/1‑122 (MQ=255)
                                                                                                                                    aaCCGCGTCAGTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAt  <  2:397275/122‑1 (MQ=255)
                                                                                                                                        gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATg                                <  1:673246/88‑1 (MQ=255)
                                                                                                                                        gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATg                                >  2:673246/1‑88 (MQ=255)
                                                                                                                                        gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAt              >  1:376320/1‑106 (MQ=255)
                                                                                                                                        gcgTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAAt              <  2:376320/106‑1 (MQ=255)
                                                                                                                                              |                                                                                                               
TTCTGGCAGCAATTGAAGAAGTGATTGCTCACGGGCCGAAAACACCTGATATGAAAGGCAATGCCACCACGCCACAGGTTGCCGACGCGATTTGCAAAATTATTTTGCGTTAAGGTCAAACCAGTTTATTTGAACCGCGTCACTGACGCGGTTTTTTTATTCGTTCTTTGCAGTAAATAACCTGCGTCATTTCACCTTTTATTGTTTCCGTTTCGTGTTTTATGGCTTTCCGTATTCTTAATTGTTTAATTTAT  >  NC_000913/1882885‑1883138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A