Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F5 I0 R1
|
408 |
67.6 |
2494072 |
96.8% |
2414261 |
139.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_000913 |
2,117,893 |
T→C |
6.6% |
N171S (AAC→AGC) |
wcaK ← |
colanic acid biosynthesis protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_000913 | 2,117,893 | 0 | T | C | 6.6%
| 193.2
/ 3.2
| 60 | N171S (AAC→AGC) | wcaK | colanic acid biosynthesis protein |
| Reads supporting (aligned to +/- strand): ref base T (27/29); new base C (2/2); total (29/31) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01 |
GTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC > NC_000913/2117754‑2118035
|
gTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGtt > 1:46916/1‑149 (MQ=255)
tcttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTg > 4:552391/1‑149 (MQ=255)
cttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTgg < 4:196057/149‑1 (MQ=255)
ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGt > 3:402475/1‑149 (MQ=255)
ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGt > 3:152330/1‑149 (MQ=255)
ttctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGt > 3:558054/1‑149 (MQ=255)
ctGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGTTa > 3:204951/1‑149 (MQ=255)
gtgtgGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGAAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTaaa < 3:584198/149‑1 (MQ=255)
gATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCa > 2:468735/1‑149 (MQ=255)
aTCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAg < 1:486426/132‑1 (MQ=255)
aTCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAg > 2:486426/1‑132 (MQ=255)
gACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt > 1:193645/1‑149 (MQ=255)
gACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt < 3:180072/149‑1 (MQ=255)
gACCAGCCACGAGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTCCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCt > 1:507988/1‑149 (MQ=255)
aCGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACggg < 3:118572/149‑1 (MQ=255)
gACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACtgt > 3:128706/1‑149 (MQ=255)
ccATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGAcc > 2:265502/1‑149 (MQ=255)
ccATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGAcc > 4:249288/1‑149 (MQ=255)
aCTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATaaa < 4:473151/149‑1 (MQ=255)
tttGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAg < 1:337706/149‑1 (MQ=255)
ttGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc < 1:202188/149‑1 (MQ=255)
ggtggtGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCt < 4:428421/149‑1 (MQ=255)
tggtgATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCttt > 2:100100/1‑149 (MQ=255)
tgATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTc < 2:333957/149‑1 (MQ=255)
ctgcGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATaaa > 2:221116/1‑149 (MQ=255)
tgcGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAAc > 3:441322/1‑149 (MQ=255)
cGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGt < 2:390645/149‑1 (MQ=255)
cGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGt < 4:347099/149‑1 (MQ=255)
gTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTg > 4:80124/1‑149 (MQ=255)
tttCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc > 4:439697/1‑118 (MQ=255)
tttCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc < 3:439697/118‑1 (MQ=255)
catcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCaaa < 1:221116/149‑1 (MQ=255)
tcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc > 2:591669/1‑122 (MQ=255)
tcaGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGc < 1:591669/122‑1 (MQ=255)
caGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTg < 1:401952/149‑1 (MQ=255)
aGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGCCCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGc < 4:240157/149‑1 (MQ=255)
aTCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCAt < 2:507988/149‑1 (MQ=255)
aTCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCAt < 2:46916/149‑1 (MQ=255)
aaaGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGtt > 2:481981/1‑149 (MQ=255)
aaGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGCTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTc < 4:204951/149‑1 (MQ=255)
gCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc > 3:425064/1‑103 (MQ=255)
gCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATc < 4:425064/103‑1 (MQ=255)
cTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCaaa < 1:348118/149‑1 (MQ=255)
gACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACt > 3:290150/1‑149 (MQ=255)
aCCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTg > 3:448406/1‑149 (MQ=255)
gATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCgg > 4:300631/1‑149 (MQ=255)
aTTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGc > 1:359326/1‑149 (MQ=255)
gATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATaga < 3:248114/149‑1 (MQ=255)
tCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAgaga < 4:128706/149‑1 (MQ=255)
cAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGAt < 2:193645/149‑1 (MQ=255)
aGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATc < 1:100100/149‑1 (MQ=255)
aGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGAGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATc < 2:341510/149‑1 (MQ=255)
tGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGa > 4:492494/1‑149 (MQ=255)
gACCAAAAACGTAGCTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGAt < 4:558054/149‑1 (MQ=255)
cAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccg > 1:606728/1‑149 (MQ=255)
cAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccg > 2:477706/1‑149 (MQ=255)
aaaaaCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc < 3:300631/149‑1 (MQ=255)
aaaaaCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATccgc < 1:468735/149‑1 (MQ=255)
aCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc < 3:552391/149‑1 (MQ=255)
aCGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAc > 3:517593/1‑149 (MQ=255)
|
GTCTTCTGTGTGGTGATCGACCAGCCACGCGGTATCGACGCCATGTTCCACTTTTGCGGTGGTGATATTGCTGCGTTTCATCAGATCAAAGCTGACCGATTCGCGCAGGATCAGCGCGTCGCAGTGACCAAAAACGTAGTTCGCCAGTTGGTTAAATTGCTCATCCTGGAACGGGCCAACACTGTGACCAATCATAAACAGCGGCTTTTTCGCCATAAACGTGCAAAGTGCATGTTCAAACTGCGGCACGCCATAGAGATCGACAAAAAACGATCCGCCGAC > NC_000913/2117754‑2118035
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
