Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A51 F1 I1 R1
|
20 |
58.0 |
3778966 |
96.2% |
3635365 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,561,758 |
G→A |
S993S (AGC→AGT) |
hsdR ← |
endonuclease R |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,561,758 | 0 | G | A | 100.0%
| 184.0
/ NA
| 48 | S993S (AGC→AGT) | hsdR | endonuclease R |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (20/28); total (20/28) |
GTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACGCTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGTTTTC > REL606/4561688‑4561824
|
gTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTcac > 1:1631538/1‑76 (MQ=255)
ttGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTcacc > 2:1490073/1‑76 (MQ=255)
gCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTcaccac > 2:443185/1‑76 (MQ=255)
cACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACt > 2:382322/1‑76 (MQ=255)
aCCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACtt < 1:761897/76‑1 (MQ=255)
aCCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACtt < 1:1295998/76‑1 (MQ=255)
aCCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACtt > 1:1885938/1‑76 (MQ=255)
cGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCAtcg > 1:1551449/1‑76 (MQ=255)
cGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCAtcg > 2:118001/1‑76 (MQ=255)
gAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCAtcgt > 2:180505/1‑76 (MQ=255)
tCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGa > 2:439977/1‑76 (MQ=255)
cAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGat < 1:1490073/76‑1 (MQ=255)
cAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGat < 2:46546/76‑1 (MQ=255)
gCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGa < 1:1153880/76‑1 (MQ=255)
cTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAg < 2:1631538/76‑1 (MQ=255)
cTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAg > 2:237948/1‑76 (MQ=255)
cTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAg > 2:1290024/1‑76 (MQ=255)
cTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAg > 2:483617/1‑76 (MQ=255)
ttCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAgg < 2:1848603/76‑1 (MQ=255)
tCGGGGAAATCCTGCGGCGTGACGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGa < 1:1046089/76‑1 (MQ=255)
tCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGa < 2:1191174/76‑1 (MQ=255)
cGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGaa > 1:926833/1‑76 (MQ=255)
gAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAg < 1:1748232/76‑1 (MQ=255)
gAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAg > 2:474775/1‑76 (MQ=255)
gAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATc < 1:641975/76‑1 (MQ=255)
tCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGgcg < 1:1824223/76‑1 (MQ=255)
ggcgTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGcatcat < 2:1400916/76‑1 (MQ=255)
cgTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTc < 2:906583/76‑1 (MQ=255)
cgTGTCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTc < 1:1182151/76‑1 (MQ=255)
tgtCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAg < 2:737911/76‑1 (MQ=255)
tgtCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAg < 1:1594009/76‑1 (MQ=255)
tgtCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAg < 2:1845162/76‑1 (MQ=255)
gtCGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAgg < 1:1794037/76‑1 (MQ=255)
cGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGgtt > 1:688787/1‑76 (MQ=255)
cGTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGgtt > 1:1697496/1‑76 (MQ=255)
gTAATCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGgttg > 1:204364/1‑76 (MQ=255)
aaTCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGgttgtt > 2:1104826/1‑76 (MQ=255)
aTCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGttgttg < 1:57437/76‑1 (MQ=255)
aTCACCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGttgttg < 1:763226/76‑1 (MQ=255)
aCCGTACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATg > 1:153588/1‑76 (MQ=255)
tACAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCgg < 1:1757044/76‑1 (MQ=255)
aCAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGt < 2:601566/76‑1 (MQ=255)
cAGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGtt > 1:1814866/1‑76 (MQ=255)
aGCGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGttt < 2:82124/76‑1 (MQ=255)
cGATTTTACACTCACCTCTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGTTTTc < 2:1671856/76‑1 (MQ=255)
cGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGTTTTc < 2:334052/76‑1 (MQ=255)
cGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGTTTTc < 1:443185/76‑1 (MQ=255)
cGATTTTACACTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGTTTTc < 2:1147284/76‑1 (MQ=255)
|
GTTGCACCAGCGAGTCAAAGGCTTCGAGGAAATCCTGCGGCGTGTCGTAATCACCGTACAGCGATTTTACGCTCACCACTTCATCGTCGATATCGAGGAAGATCGGCGCATCATTCAGGTTGTTGATGTCGGTTTTC > REL606/4561688‑4561824
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
