Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A33 F1 I1 R1	22	54.4	3455062	95.8%	3309949	75.9

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	2,055,515	G→T	N194K (AAC→AAA)	cpsG ←	phosphomannomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	2,055,515	0	G	T	93.8%	45.6 / ‑2.0	16	N194K (AAC→AAA)	cpsG	phosphomannomutase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (7/8); total (7/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

TAGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCGTTAAAGCGGGCTTCGATAGCGTCCACCACCGGACCCGCTGCGCCG  >  REL606/2055442‑2055560

tAGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCttt                                             >  1:1316461/1‑76 (MQ=21)

 aGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTa                                            <  1:837282/76‑1 (MQ=21)

    aaTACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAg                                         >  1:1419726/1‑76 (MQ=21)

      tACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCg                                       >  2:1249168/1‑76 (MQ=21)

        ccGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCggg                                     >  1:728035/1‑76 (MQ=21)

        ccGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCggg                                     >  1:807131/1‑76 (MQ=21)

           ttGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCtt                                  <  2:508806/76‑1 (MQ=255)

           ttGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCtt                                  <  2:10786/76‑1 (MQ=255)

            tGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTc                                 >  2:823410/1‑76 (MQ=255)

            tGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTc                                 >  2:1454338/1‑76 (MQ=255)

             ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:753562/76‑2 (MQ=255)

             ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:590630/76‑2 (MQ=255)

             ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:243554/76‑2 (MQ=255)

             ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                <  1:229518/76‑2 (MQ=255)

             ggggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTca                                >  1:1666040/1‑75 (MQ=255)

               ggAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCTTTAAAGCGGGCTTCAAt                              <  1:1143633/76‑1 (MQ=255)

                                           gttGATTAATTCCGCCGGAGCGCCGAGGGCGTTAAAGCGGGCTTCGATAGCGTCCACCACCGGACCCGCTGCGCCg  <  2:657806/75‑1 (MQ=255)

TAGGAATACCGTTGGGGAAATTGCCGTCCGGCGTGTTGTGCACTTTGATTAATTCCACCGGAGCGCCGAGGGCGTTAAAGCGGGCTTCGATAGCGTCCACCACCGGACCCGCTGCGCCG  >  REL606/2055442‑2055560

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A