Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A33 F1 I1 R1
|
22 |
54.4 |
3455062 |
95.8% |
3309949 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,881 |
C→T |
R52H (CGT→CAT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,881 | 0 | C | T | 98.1%
| 199.7
/ ‑5.5
| 52 | R52H (CGT→CAT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (24/27); total (25/27) |
| Fisher's exact test for biased strand distribution p-value = 4.81e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGACGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAAT > REL606/3497809‑3497951
|
gACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAgatgat < 2:335534/76‑1 (MQ=255)
cAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATcg < 2:1115537/76‑1 (MQ=255)
tagtaACCCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAGCCAGCGCTCCACGAGATGATCGCGca < 1:357302/76‑1 (MQ=255)
tagtaAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGca < 1:875270/76‑1 (MQ=255)
gtaAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGcaca < 1:1541655/76‑1 (MQ=255)
taAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAg > 2:791766/1‑76 (MQ=255)
taAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAg < 1:571943/76‑1 (MQ=255)
taAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAg > 1:411465/1‑76 (MQ=255)
taAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAg > 1:259319/1‑76 (MQ=255)
aaaCCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGc > 2:1154084/1‑76 (MQ=255)
aaCCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCa > 1:1654498/1‑76 (MQ=255)
cTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAAt > 1:811466/1‑76 (MQ=255)
gACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATaa < 1:205518/76‑1 (MQ=255)
gACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATaa < 1:990832/76‑1 (MQ=255)
gACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATaa > 2:308979/1‑76 (MQ=255)
gACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATaa < 2:1470646/76‑1 (MQ=255)
cGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACg > 2:1144499/1‑76 (MQ=255)
aGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGtt < 1:443104/76‑1 (MQ=255)
aGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGtt > 1:1472418/1‑76 (MQ=255)
gTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTg < 1:243126/76‑1 (MQ=255)
tttCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGc > 2:1658674/1‑76 (MQ=255)
tttCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGc < 1:595857/76‑1 (MQ=255)
tttCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGc < 1:185870/76‑1 (MQ=255)
ttCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCg > 1:1470994/1‑76 (MQ=255)
tGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCa < 1:440844/76‑1 (MQ=255)
tGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCa < 2:1161569/76‑1 (MQ=255)
gCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAg < 2:40524/76‑1 (MQ=255)
gCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAg < 2:1422008/76‑1 (MQ=255)
aaCTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCAtt > 2:262702/1‑76 (MQ=255)
aCTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTc > 1:1421183/1‑76 (MQ=255)
cTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCa < 1:574878/76‑1 (MQ=255)
gggCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATg > 2:1479995/1‑76 (MQ=255)
gggCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATg > 2:700534/1‑76 (MQ=255)
aCGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTa < 2:1088018/76‑1 (MQ=255)
aCGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTa < 1:882901/76‑1 (MQ=255)
cGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTAt < 2:955007/76‑1 (MQ=255)
tGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGcg < 1:504447/76‑1 (MQ=255)
tGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGcg > 1:1266966/1‑76 (MQ=255)
gAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGcga < 1:56070/76‑1 (MQ=255)
gAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGcga < 2:1253270/76‑1 (MQ=255)
aCGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGcgacg < 1:406430/76‑1 (MQ=255)
cAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGAGCGGGt > 2:404340/1‑76 (MQ=255)
cAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGt > 2:1266177/1‑76 (MQ=255)
cAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGt > 2:1147432/1‑76 (MQ=255)
aGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTc > 2:1540848/1‑76 (MQ=255)
gcTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCtt < 1:1678691/76‑1 (MQ=255)
cTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCttt < 2:1054610/76‑1 (MQ=255)
cTCCACGAGACGATCCGGCACAGCAAATAACGTTGCGTTCAGCCATTCAGGTTTAGTGGCGAAGACCGGGTCGttt > 2:1590011/1‑76 (MQ=255)
ccACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTcc > 1:360933/1‑76 (MQ=255)
ccACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTcc > 1:1638450/1‑76 (MQ=255)
ccACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTcc > 1:1600543/1‑76 (MQ=255)
ccACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTcc < 1:1415123/76‑1 (MQ=255)
cGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAAt > 1:581174/1‑76 (MQ=255)
|
GACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGACGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAAT > REL606/3497809‑3497951
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
