Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A143 F1 I1 R1
|
16 |
39.8 |
2536279 |
96.2% |
2439900 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,881 |
C→T |
R52H (CGT→CAT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,881 | 0 | C | T | 100.0%
| 141.9
/ NA
| 38 | R52H (CGT→CAT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (19/19); total (19/19) |
CGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGACGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAA > REL606/3497808‑3497956
|
cGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGa > 2:607613/1‑76 (MQ=255)
cAGGTAGTAACCCTGACAAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCACCACGAGATGATcg > 1:101595/1‑76 (MQ=255)
cAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATcg < 2:228380/76‑1 (MQ=255)
gtagtaAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCgcgc > 2:416195/1‑76 (MQ=255)
taAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAg < 2:840150/76‑1 (MQ=255)
aaaCCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGc > 2:616593/1‑76 (MQ=255)
aaaCCTGACGAGATTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGAACAGc > 2:47381/1‑76 (MQ=255)
cTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGGTCGCGCACAGCAAAt < 2:542662/76‑1 (MQ=255)
tGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTACGCAGCGCTCCACGNGATGAGCTCGNACACCAAATa < 2:1242018/76‑1 (MQ=255)
gACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATaa < 2:634897/76‑1 (MQ=255)
gACGAGTTTCTGGCCACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGTTCGCGCACAGCAAATaa < 1:98364/76‑1 (MQ=255)
tttCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGc > 2:777522/1‑76 (MQ=255)
tCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGt < 1:770132/76‑1 (MQ=255)
cTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGtt > 2:807200/1‑76 (MQ=255)
tGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCa < 2:835495/76‑1 (MQ=255)
gCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAg < 1:329477/76‑1 (MQ=255)
aCAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCa > 2:1226432/1‑76 (MQ=255)
cAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCAt > 2:899376/1‑76 (MQ=255)
tGGGCACGGTATGAACGGAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGAGTTCAGCCATTCAt > 1:98282/1‑76 (MQ=255)
gggCACGGTTTGAAGGTGATCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATg < 1:55853/76‑1 (MQ=255)
cACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGttt > 1:885259/1‑76 (MQ=255)
cACGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGttt < 1:467104/76‑1 (MQ=255)
cGGTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTAt < 2:473711/76‑1 (MQ=255)
gTTTGAATGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTg < 1:763683/76‑1 (MQ=255)
gTTTGAACGTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTg > 1:614113/1‑76 (MQ=255)
gTAACCAGCGCTCCTCGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTACTGGCgacgac < 1:104281/76‑1 (MQ=255)
gTAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCgacgac < 2:259211/76‑1 (MQ=255)
tAACCAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGAcc > 2:745253/1‑76 (MQ=255)
aaCCCGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTTATGTTTATTGGCGACGACCg < 2:98282/76‑1 (MQ=255)
aCCAGCGCACCACGAGATGATCGCGCACAGCATATGACGTTGCGTTGAGCTATTCATGTTTATGGGCGACGACCgg > 2:23561/1‑76 (MQ=255)
cAGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGt > 2:1136923/1‑76 (MQ=255)
aGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCggggc > 1:745664/1‑74 (MQ=255)
aGCGCTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTc < 2:1182755/76‑1 (MQ=255)
cTCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGGCCgtt > 2:586989/1‑76 (MQ=255)
tCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTc < 1:297978/76‑1 (MQ=255)
tCCACGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTc > 1:245141/1‑76 (MQ=255)
cGAGATGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCGTTTCCAAt > 2:1123586/1‑76 (MQ=255)
gagaTGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATc < 2:413738/76‑1 (MQ=255)
agaTGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCg > 2:265460/1‑76 (MQ=255)
cGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTaa < 2:337414/76‑1 (MQ=255)
|
CGACAGGTAGTAAACCTGACGAGTTTCTTGCGACAACTGGGCACGGTTTGAACGTAACCAGCGCTCCACGAGACGATCGCGCACAGCAAATAACGTTGCGTTCAGCCATTCATGTTTATTGGCGACGACCGGGTCCTTTCCAATCGTAA > REL606/3497808‑3497956
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
