Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A71 F1 I1 R1
|
18 |
59.4 |
3821164 |
95.9% |
3664496 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,781,691 |
A→T |
I179F (ATC→TTC) |
ygcN → |
predicted oxidoreductase with FAD/NAD(P)‑binding domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,781,691 | 0 | A | T | 94.9%
| 214.8
/ 2.1
| 60 | I179F (ATC→TTC) | ygcN | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
| Reads supporting (aligned to +/- strand): ref base A (0/3); new base T (24/32); total (25/35) |
| Fisher's exact test for biased strand distribution p-value = 2.64e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.16e-01 |
AGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAG > REL606/2781618‑2781763
|
aGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTc > 2:1652452/1‑76 (MQ=255)
aGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTc < 2:924420/76‑1 (MQ=255)
ggTGCCAGCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCa < 2:163009/76‑1 (MQ=255)
ggTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCa < 1:913599/76‑1 (MQ=255)
gTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCaa < 1:209971/76‑1 (MQ=255)
gCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGGTCAaag > 1:1003959/1‑76 (MQ=255)
aaCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAaagaag < 2:1620248/76‑1 (MQ=255)
aaCAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGGTGGGGGTCAaagaag > 2:455313/1‑76 (MQ=255)
cAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTg > 1:1388436/1‑76 (MQ=255)
cAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTg > 2:1480305/1‑76 (MQ=255)
aGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGc < 1:1127846/76‑1 (MQ=255)
cGTCTTGGCTTACCGTCACGGGTCAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTg < 1:750466/76‑1 (MQ=255)
cGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTg > 1:475847/1‑76 (MQ=255)
cGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTg < 2:112217/76‑1 (MQ=255)
tCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTc < 2:239495/76‑1 (MQ=255)
cTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGGGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGc > 2:642516/1‑76 (MQ=255)
cTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGtaag < 2:1290876/76‑4 (MQ=255)
cTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGc > 1:1782873/1‑76 (MQ=255)
tGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCt < 1:1427360/76‑1 (MQ=255)
ggCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTg > 1:792393/1‑76 (MQ=255)
gCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTgg > 1:1225636/1‑76 (MQ=255)
cTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGa < 1:1095780/76‑1 (MQ=255)
tGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGaa > 1:404997/1‑76 (MQ=255)
gAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGaaa > 2:1262570/1‑76 (MQ=255)
gAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGaaa < 1:324119/76‑1 (MQ=255)
aaCGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAAc < 1:800220/76‑1 (MQ=255)
tCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGGAACATcc > 2:874957/1‑76 (MQ=255)
cACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCg > 1:355882/1‑76 (MQ=255)
cACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCg > 2:980630/1‑76 (MQ=255)
cGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCt < 1:1862086/76‑1 (MQ=255)
cGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCt > 2:1836547/1‑76 (MQ=255)
gTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTAtt < 1:1366747/76‑1 (MQ=255)
tAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTga < 2:1603477/76‑1 (MQ=255)
tAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTga < 1:1480305/76‑1 (MQ=255)
gTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaag < 2:877571/76‑1 (MQ=255)
gACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaagaa < 1:165918/76‑1 (MQ=255)
gACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaagaa > 1:1176224/1‑76 (MQ=255)
gACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTgaagaa > 2:5838/1‑76 (MQ=255)
cTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACg > 2:1337833/1‑76 (MQ=255)
cTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACg > 1:1031149/1‑76 (MQ=255)
cTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACg < 2:1614793/76‑1 (MQ=255)
gTCCTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGttt < 1:115368/76‑1 (MQ=255)
ccTGCTGGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTc < 1:211820/76‑1 (MQ=255)
ctgctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCa > 2:562228/1‑76 (MQ=255)
tgctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCAt < 1:507282/76‑1 (MQ=255)
gctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATc > 2:159377/1‑76 (MQ=255)
ctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCt < 2:1081275/76‑1 (MQ=255)
ctgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCt < 1:1784602/76‑1 (MQ=255)
tgGCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTg > 2:87748/1‑76 (MQ=255)
ggCGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTgg > 1:1384611/1‑76 (MQ=255)
cGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGgag < 1:109430/76‑1 (MQ=255)
cGAAGCGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGgag < 1:1295624/76‑1 (MQ=255)
cGATGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATaa < 1:1116405/76‑1 (MQ=255)
aTGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACg > 2:1049281/1‑76 (MQ=255)
tGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGa > 1:1552073/1‑76 (MQ=255)
tGGCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGa < 2:264645/76‑1 (MQ=255)
gCGTTGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAg < 2:404997/76‑1 (MQ=255)
gTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGgc < 2:1677833/76‑1 (MQ=255)
tGGGGTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGgcgc < 1:1735830/76‑1 (MQ=255)
ggggTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCa < 1:1457294/76‑1 (MQ=255)
cgggTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCa > 1:792121/2‑76 (MQ=255)
gggTTCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAg < 1:1596110/76‑1 (MQ=255)
|
AGGTGCCAACAGCGTCCTGGCTGAACGTCACGGGTTAGTGACTCGTCCTGCTGGCGAAGCGATGGCGTTGGGGATCAAAGAAGTGCTGTCGCTGGAAACATCCGCTATTGAAGAACGTTTTCATCTGGAGAATAACGAAGGCGCAG > REL606/2781618‑2781763
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
