breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 664,688 | +T | intergenic (‑79/‑47) | gltL ← / → insJ‑2 | ATP‑binding protein of glutamate/aspartate transport system; b0652_1/IS150 hypothetical protein |
RA | 1,305,155 | A→C | V362G (GTG→GGG) | cls ← | cardiolipin synthetase |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
RA | 1,690,717 | +GT | coding (755/1503 nt) | ydgR → | putative tripeptide transporter permease |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 1,886,235 | C→A | intergenic (‑65/+604) | yobF ← / ← yebO | hypothetical protein/hypothetical protein |
JC | 2,103,918 | (CCAG)7→9 | coding (185/216 nt) | ECB_01992 → | hypothetical protein |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 2,781,691 | A→T | I179F (ATC→TTC) | ygcN → | predicted oxidoreductase with FAD/NAD(P)‑binding domain |
RA | 3,252,016 | C→T | G249S (GGT→AGT) | nusA ← | transcription elongation factor NusA |
RA | 3,328,104 | A→G | V292A (GTG→GCG) | mreB ← | cell wall structural complex MreBCD, actin‑like component MreB |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,911,972 | (T)7→6 | intergenic (‑173/‑150) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
RA | 4,096,973 | A→C | F200L (TTT→TTG) | glpF ← | glycerol facilitator |
RA | 4,163,452 | A→G | D866G (GAT→GGT) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 589612–590469 | 593416 | 2948–3805 | 20 [17] | [18] 19 | [insK‑1]–[fepA] | [insK‑1],[insB‑7],insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
* | * | ÷ | REL606 | 593573 | 619829–619116 | 25544–26257 | 19 [16] | [18] 19 | [fepA]–[insA‑8] | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8] |