breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 664,688 +T intergenic (‑79/‑47) gltL ← / → insJ‑2 ATP‑binding protein of glutamate/aspartate transport system; b0652_1/IS150 hypothetical protein
RA 1,305,155 A→C V362G (GTG→GGG)  cls ← cardiolipin synthetase
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,690,717 +GT coding (755/1503 nt) ydgR → putative tripeptide transporter permease
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,886,235 C→A intergenic (‑65/+604) yobF ← / ← yebO hypothetical protein/hypothetical protein
JC 2,103,918 (CCAG)7→9 coding (185/216 nt) ECB_01992 → hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,781,691 A→T I179F (ATC→TTC)  ygcN → predicted oxidoreductase with FAD/NAD(P)‑binding domain
RA 3,252,016 C→T G249S (GGT→AGT)  nusA ← transcription elongation factor NusA
RA 3,328,104 A→G V292A (GTG→GCG)  mreB ← cell wall structural complex MreBCD, actin‑like component MreB
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,972 (T)7→6 intergenic (‑173/‑150) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 4,096,973 A→C F200L (TTT→TTG glpF ← glycerol facilitator
RA 4,163,452 A→G D866G (GAT→GGT)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 589612–590469 593416 2948–3805 20 [17] [18] 19 [insK‑1]–[fepA] [insK‑1],[insB‑7],insA‑7,insK‑1,hokE,insL‑3,entD,[fepA]
* * ÷ REL606 593573 619829–619116 25544–26257 19 [16] [18] 19 [fepA]–[insA‑8] [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,[insA‑8]