breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6062,296,3290AC46.7% 50.5 / 52.7 60intergenic (‑149/‑66)inaA/yfaHhypothetical protein/hypothetical protein
*REL6064,393,0610TG39.5% 57.1 / 13.8 38V211G (GTG→GGG) aidBisovaleryl CoA dehydrogenase
*REL6061,688,9900AC33.3% 90.4 / 10.9 46T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase (endonuclease III)
*REL6063,492,0820TG33.3% 122.7 / 11.5 60V490G (GTG→GGG) glpDsn‑glycerol‑3‑phosphate dehydrogenase, aerobic, FAD/NAD(P)‑binding
*REL6061,875,4830GC31.3% 182.6 / 100.9 100intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL6062,631,7720AC30.2% 87.7 / 11.5 43intergenic (‑267/‑141)rpoE/nadBRNA polymerase, sigma 24 (sigma E) factor/L‑aspartate oxidase
*REL6061,747,9030GT29.2% 199.4 / 72.1 89A8S (GCA→TCA) ydiLhypothetical protein
*REL6062,448,0980TG29.2% 99.6 / 14.1 49F278V (TTT→GTT) nupCnucleoside (except guanosine) transporter
*REL6061,004,1060AC26.7% 129.1 / 14.5 60intergenic (‑32/+570)ompF/asnSouter membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
*REL6061,484,1050AG26.2% 86.6 / 12.8 42G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*REL606154,4970TG23.1% 131.4 / 10.6 52A191A (GCA→GCCyadLpredicted fimbrial‑like adhesin protein
*REL6061,416,8380TG22.5% 167.2 / 11.6 71intergenic (+1/‑4)ECB_01334/ECB_01335putative Lysozyme/conserved hypothetical protein
*REL6062,417,4950TA20.3% 176.5 / 10.8 59L24F (TTA→TTTemrKEmrKY‑TolC multidrug resistance efflux pump, membrane fusion protein component

Marginal new junction evidence (lowest skew 10 of 270 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 = 2039480117 (1.970)15 (0.280) 12/134 3.7 17.2% coding (215/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 = 2039680 40 (0.760)coding (15/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 358233692 (1.550)16 (0.290) 12/140 3.9 16.3% coding (356/1224 nt) yhiS hypothetical protein
?REL606 = 3582485 78 (1.410)coding (505/1224 nt) yhiS hypothetical protein
* ? REL606 2041682 =56 (0.940)13 (0.260) 10/128 4.0 16.4% coding (1139/1428 nt) wzx O antigen translocase (Wzx)
?REL606 2041834 = 85 (1.680)coding (987/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 2041245 =50 (0.840)14 (0.250) 11/140 4.1 17.6% coding (139/1113 nt) vioA VioA, involved in dTDP‑N‑acetylviosamine synthesis
?REL606 2041495 = 84 (1.520)coding (1326/1428 nt) wzx O antigen translocase (Wzx)
* ? REL606 = 3021002120 (2.020)13 (0.240) 9/136 4.6 12.5% coding (482/933 nt) ECB_02820 hypothetical protein
?REL606 = 3021158 73 (1.360)coding (326/933 nt) ECB_02820 hypothetical protein
* ? REL606 2038074 =73 (1.230)12 (0.230) 9/134 4.6 16.7% coding (57/1176 nt) wzy O antigen polymerase (Wzy)
?REL606 2038286 = 55 (1.040)coding (663/822 nt) wbbB putative glycosyltransferase WbbB
* ? REL606 3123465 =78 (1.310)10 (0.180) 9/140 4.8 12.4% coding (749/750 nt) yqiH predicted periplasmic pilin chaperone
?REL606 3123649 = 69 (1.250)coding (182/1065 nt) yqiI hypothetical protein
* ? REL606 2039188 =35 (0.590)11 (0.200) 9/140 4.8 14.1% coding (507/747 nt) wbbA putative glycosyltransferase WbbA
?REL606 2039561 = 102 (1.840)coding (134/747 nt) wbbA putative glycosyltransferase WbbA
* ? REL606 = 160083828 (0.470)14 (0.250) 9/140 4.8 34.9% coding (34/267 nt) ydfK predicted DNA‑binding transcriptional regulator
?REL606 = 1600968 NA (NA)coding (164/267 nt) ydfK predicted DNA‑binding transcriptional regulator
* ? REL606 1425700 =NA (NA)13 (0.230) 9/140 4.8 34.1% coding (164/267 nt) ynaE predicted DNA‑binding transcriptional regulator
?REL606 1425830 = 27 (0.460)coding (34/267 nt) ynaE predicted DNA‑binding transcriptional regulator