Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A71 F1 I1 R1
|
18 |
59.4 |
3821164 |
95.9% |
3664496 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,252,016 |
C→T |
G249S (GGT→AGT) |
nusA ← |
transcription elongation factor NusA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,252,016 | 0 | C | T | 100.0%
| 164.0
/ NA
| 42 | G249S (GGT→AGT) | nusA | transcription elongation factor NusA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (18/24); total (18/24) |
TATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTG > REL606/3251944‑3252087
|
tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTAc < 1:1150173/76‑1 (MQ=255)
tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTAc > 1:1280889/1‑76 (MQ=255)
tatCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTAc > 1:1767097/1‑76 (MQ=255)
tCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCg < 1:1526992/76‑1 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCgg < 1:1040703/76‑1 (MQ=255)
cGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCgg > 1:589202/1‑76 (MQ=255)
aTACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGAt < 2:1796385/76‑1 (MQ=255)
tACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATc > 1:618733/1‑76 (MQ=255)
cTCGCCCCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATa < 1:1281170/76‑1 (MQ=255)
cTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATa < 1:23664/76‑1 (MQ=255)
tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc < 1:295848/76‑1 (MQ=255)
tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc > 2:39545/1‑76 (MQ=255)
tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc > 1:469222/1‑76 (MQ=255)
tCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATAc < 1:1423490/76‑1 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt < 1:328371/76‑1 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt < 2:1785676/76‑1 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt < 1:805678/76‑1 (MQ=255)
cACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGttt > 2:1528949/1‑76 (MQ=255)
aCCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTa < 1:646461/76‑1 (MQ=255)
cccAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTAt < 1:428934/76‑1 (MQ=255)
cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCg < 2:864752/76‑1 (MQ=255)
cAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCg > 1:1228011/1‑76 (MQ=255)
aGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGt > 2:1868819/1‑76 (MQ=255)
tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGtttt < 2:1835131/76‑1 (MQ=255)
tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGtttt < 2:351875/76‑1 (MQ=255)
tAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGtttt > 2:674244/1‑76 (MQ=255)
aGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTc < 2:1768789/76‑1 (MQ=255)
cTGAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtt < 1:1138029/76‑1 (MQ=255)
gAACACGCGCGCCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGAtttt < 2:1127730/76‑1 (MQ=255)
cgcCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACgaga < 2:122749/76‑1 (MQ=255)
gcCACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGaa < 1:538044/76‑1 (MQ=255)
ccACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAc > 2:138832/1‑76 (MQ=255)
cACGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAcc < 2:35071/76‑1 (MQ=255)
aCGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAAccc < 2:876386/76‑1 (MQ=255)
cGCATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCg > 1:921747/1‑76 (MQ=255)
cATACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGa > 1:1406635/1‑76 (MQ=255)
aTACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGAt > 2:385289/1‑76 (MQ=255)
tACCTACGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATc < 2:1557665/76‑1 (MQ=255)
aCGCAAGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAg > 1:1323360/1‑76 (MQ=255)
aaGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgc > 2:1724667/1‑76 (MQ=255)
aaGCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgc > 1:625042/1‑76 (MQ=255)
gCACTTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCgctg > 1:676021/1‑76 (MQ=255)
|
TATCGATACGCTCGCCACCCAGTTCAGTAGACACCGCCTGAACACGCGCGCCACGCATACCTACGCAAGCACCTACCGGATCGATACGTTTATCGTTGGTTTTCACCGCGATTTTCGCACGAGAACCCGGATCGCGAGCCGCTG > REL606/3251944‑3252087
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
