Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A71 F1 I1 R1
|
18 |
59.4 |
3821164 |
95.9% |
3664496 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,104 |
A→G |
V292A (GTG→GCG) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,104 | 0 | A | G | 97.6%
| 146.8
/ ‑3.7
| 41 | V292A (GTG→GCG) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (30/10); total (31/10) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.03e-01 |
GACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTC > REL606/3328034‑3328175
|
gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATg < 2:833725/76‑1 (MQ=255)
gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATg > 2:222058/1‑76 (MQ=255)
gACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATg < 2:1881700/76‑1 (MQ=255)
aCTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGc > 2:700989/1‑76 (MQ=255)
aCTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGGGAGCGCCACGc > 1:948639/1‑76 (MQ=255)
cTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGcc > 2:105969/1‑76 (MQ=255)
aTGCCGGTTTCTCCCATACACAAACGGCCAAGTTTCCGCGGCAGTGCCCCCCCGCC‑GGTGAGCGCCATGCCGCGCt < 2:603796/76‑1 (MQ=255)
gCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCg > 1:1703315/1‑76 (MQ=255)
gTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGgaga > 2:1102326/1‑76 (MQ=255)
tttCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGAt > 2:1843569/1‑76 (MQ=255)
tttCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGAt > 1:1392224/1‑76 (MQ=255)
tcttcCATAAACAACCGGTCAAGGTTACGCAGCAGTGCGCCCCCCCC‑GGTGAGCGCCATGCCGCGCTCGGAGATGt < 2:1222138/76‑1 (MQ=255)
cttcCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTc > 1:1444018/1‑76 (MQ=255)
cccATAAACAACCGCTGAGGGTTACGCACGTGTGCGCCCCCACG‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCgg < 1:1197248/75‑1 (MQ=255)
aTTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAg > 1:1332102/1‑76 (MQ=255)
ttAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGc > 2:1337841/1‑76 (MQ=255)
tAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGcc > 2:625103/1‑76 (MQ=255)
aCAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAg > 2:1019745/1‑76 (MQ=255)
cAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGt > 2:1371009/1‑76 (MQ=255)
aaccGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGtt < 1:1787046/73‑1 (MQ=255)
aaccGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGtt < 2:1469888/73‑1 (MQ=255)
aaaCGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGCTGTCGGAAGCCAGtt > 2:877051/1‑76 (MQ=255)
aaaCGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTGGGTAGCCGGtt > 2:1354505/1‑76 (MQ=255)
agcGGTCAGGGTTACGCGGCGGTGCGCCCCCCCC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTc < 2:1296728/74‑1 (MQ=255)
gTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc > 2:230993/1‑76 (MQ=255)
tCAAGGTTACGCACCAGTGCCCCCCCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggcg < 1:928888/76‑1 (MQ=255)
cAAGTTTACGCAGCAGTGCGCCCCCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggcgg < 1:1909390/76‑1 (MQ=255)
cAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGGCGGAAGCCAGTTCcggcgg > 2:1237874/1‑76 (MQ=255)
aaGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg > 1:136305/1‑76 (MQ=255)
aaGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg > 2:1511892/1‑76 (MQ=255)
aaGGTTACGCAGCAGCGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg < 2:696178/76‑1 (MQ=255)
aGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc > 2:1603991/1‑76 (MQ=255)
ggTTACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGcc > 1:1002037/1‑75 (MQ=255)
ttACGCAGCGGTGCGCCACCGCC‑GGTGGGCGCAATGCCGCGCGCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACt < 2:1464575/76‑1 (MQ=255)
ttACGCAGCAGTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGccat > 2:1615159/1‑73 (MQ=255)
caCGCAGCGGGGCGCCCCCGCG‑GGTGGGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTg < 1:1546808/75‑1 (MQ=255)
cagcagTGCGCCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTcc > 2:1043607/1‑76 (MQ=255)
agcagGGCGCCACCCCC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCa < 2:1209450/76‑1 (MQ=255)
cagTGCGCCCCCCCC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGt < 2:1281002/76‑1 (MQ=255)
caggcgcCCCCCCCGGGTGCGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGt < 2:460607/73‑1 (MQ=255)
ggcgcCACCACC‑GGTGACCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCa < 2:1669443/75‑1 (MQ=255)
gcgcCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCaa > 2:55460/1‑76 (MQ=255)
gcCACCACC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACCGTTCCCGTGCaaac > 2:1587692/1‑74 (MQ=255)
caccacC‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAt > 2:1449430/1‑76 (MQ=255)
ccccc‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTa < 2:1551708/73‑1 (MQ=255)
c‑GGTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAcccc > 2:328811/1‑74 (MQ=255)
gTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCCGTGCAACCATTACCgcgc > 2:45322/1‑76 (MQ=255)
gTGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCgcgc < 2:1408391/76‑1 (MQ=255)
tGAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCt > 2:37610/1‑76 (MQ=255)
gAGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTc > 2:616009/1‑76 (MQ=255)
gAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGGTc > 1:59271/1‑76 (MQ=255)
|
GACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACC‑GGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTACCGCGCTC > REL606/3328034‑3328175
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
