Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A11 F1 I1 R1
|
21 |
62.2 |
4089897 |
94.3% |
3856772 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,417 |
G→A |
R188C (CGC→TGC) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,417 | 0 | G | A | 98.1%
| 191.2
/ ‑3.9
| 54 | R188C (CGC→TGC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (18/34); total (18/36) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.85e-01 |
TCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATC > REL606/3328344‑3328486
|
tCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGcaca > 2:142266/1‑76 (MQ=255)
cACCGATCAGAGAACCGTAATTACGACGCACATAGTTGCTGATAGCTTCGTCGAAATGGTCACCACCAATGCTcag > 2:1986696/1‑75 (MQ=255)
ccGATCAGAGAACCGTAATTACGACGCAGATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACag < 1:306423/76‑1 (MQ=255)
ccGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACag < 1:909823/76‑1 (MQ=255)
cGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACaga < 1:680273/76‑1 (MQ=255)
agagAACTGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAgagg < 2:821498/76‑1 (MQ=255)
agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAgagg < 1:1071013/76‑1 (MQ=255)
agagAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAgagg > 2:423653/1‑76 (MQ=255)
gaACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGt < 2:1516035/76‑1 (MQ=255)
aaCCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTa > 2:1379402/1‑76 (MQ=255)
ccGTAATTACGNCGNACATAGTTGATCATAGCTTCGTCGAAACGGTCACCACCAATGCTCACAGAAGAGGAGTaaa < 2:306970/76‑1 (MQ=255)
ccGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTaaa < 1:1378567/76‑1 (MQ=255)
ccGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTaaa > 2:1567492/1‑76 (MQ=255)
gTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAAcc > 1:1379880/1‑76 (MQ=255)
gTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAAcc < 1:634158/76‑1 (MQ=255)
tAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACca < 2:1400054/76‑1 (MQ=255)
aaTTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACcac < 2:1854378/76‑1 (MQ=255)
aaTTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACcac < 1:489151/76‑1 (MQ=255)
aTTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACcaca < 1:945512/76‑1 (MQ=255)
tACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACAcc > 1:1336626/1‑76 (MQ=255)
acgCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTc > 1:376541/1‑76 (MQ=255)
gCACATAGTTGATGNTAGCTTCGTCGAAACGGTCACCACCAATGCACTCAGAAGAGGAGTAAACCAAACCNTTCaa < 2:2043244/76‑1 (MQ=255)
acaTAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAgg > 1:765540/1‑76 (MQ=255)
aTAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGgag < 1:773418/76‑1 (MQ=255)
tAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGgaga > 1:576525/1‑76 (MQ=255)
aGTTGATGCTAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGAt > 1:865994/1‑76 (MQ=255)
aGTTGATGATAGNTTNGTCGAAACGGTCACCACCNATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGAt < 1:1668368/76‑1 (MQ=255)
aGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACNCAGAAGAGGAGTAAACCANACNGTTCAAGGAGAt > 1:1658290/1‑76 (MQ=255)
aGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGAt < 1:904758/76‑1 (MQ=255)
gTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGANATa < 1:1823185/76‑1 (MQ=255)
gTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATa > 1:875508/1‑76 (MQ=255)
gTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATa > 1:562527/1‑76 (MQ=255)
ttGATGATAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATaa < 1:1263478/76‑1 (MQ=255)
tgatAGCTTCGGCGAAACGGTCACCACCAATGCACACTGAAGAGGAGTAAACCACACCGTTCAAGTAGATAATAGc > 1:53298/1‑76 (MQ=255)
gatAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCa < 1:1567493/76‑1 (MQ=255)
tAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAAc > 2:1297560/1‑76 (MQ=255)
tAGCTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAAc > 2:1586534/1‑76 (MQ=255)
cTTCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTc < 2:1555972/76‑1 (MQ=255)
ttCGTCGAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCa < 1:280848/76‑1 (MQ=255)
cgtcgAAACGGTCANCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGACAACAGCAACTTCAGt < 1:1748405/76‑1 (MQ=255)
gtcgAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTg < 1:1263724/76‑1 (MQ=255)
gtcgAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTg > 1:1014468/1‑76 (MQ=255)
cgAAACGGTCACCACCAATGCACACAGNAGAGGAGTAAAACACACCGTTCAAGGAGATAACAGCAACTTCAGTGGt > 1:1784116/1‑76 (MQ=255)
gAAACGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTa < 2:1265877/76‑1 (MQ=255)
aaaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac < 2:425661/76‑1 (MQ=255)
aaaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac > 2:551135/1‑76 (MQ=255)
aaaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac < 1:1140040/76‑1 (MQ=255)
aaaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac < 1:80503/76‑1 (MQ=255)
aaaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac < 1:442121/76‑1 (MQ=255)
aaaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTac < 2:1337320/76‑1 (MQ=255)
aaCGGTCACCACCAATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacc < 1:973218/76‑1 (MQ=255)
aCGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTacca < 1:686626/76‑1 (MQ=255)
ccaccaATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGa < 2:652510/76‑1 (MQ=255)
accaATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGata < 2:1482079/76‑1 (MQ=255)
ccaATGCACACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGatat < 2:905720/76‑1 (MQ=255)
caATGCACACAGACGAGGAGNAAACCACACCGTTCANGGAGATAACAGCAGCGTCAGTCGTACCACCACCGATATc < 2:2018476/76‑1 (MQ=37)
|
TCACCGATCAGAGAACCGTAATTACGACGCACATAGTTGATGATAGCTTCGTCGAAACGGTCACCACCAATGCGCACAGAAGAGGAGTAAACCACACCGTTCAAGGAGATAACAGCAACTTCAGTGGTACCACCACCGATATC > REL606/3328344‑3328486
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
