Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A71 F1 I1 R1
|
18 |
59.4 |
3821164 |
95.9% |
3664496 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,096,973 |
A→C |
F200L (TTT→TTG) |
glpF ← |
glycerol facilitator |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,096,973 | 0 | A | C | 98.2%
| 206.6
/ ‑3.7
| 54 | F200L (TTT→TTG) | glpF | glycerol facilitator |
| Reads supporting (aligned to +/- strand): ref base A (0/1); new base C (24/29); total (24/30) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.66e-01 |
TAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGG > REL606/4096900‑4097044
|
tAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCcaa < 1:1673646/76‑1 (MQ=255)
aaaGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCcaaa > 1:1307159/1‑76 (MQ=255)
aaaGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCcaaa > 1:1572527/1‑76 (MQ=255)
aGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAAcc < 1:1318256/76‑1 (MQ=255)
cGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGt < 2:1638152/76‑1 (MQ=255)
gACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTc > 2:1030783/1‑76 (MQ=255)
cATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCaa < 1:949177/76‑1 (MQ=255)
aTTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAAt < 2:1460868/76‑1 (MQ=255)
gCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATggg < 2:1081916/76‑1 (MQ=255)
gCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATggg < 2:218164/76‑1 (MQ=255)
cccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCCAgggggc > 1:243460/1‑74 (MQ=255)
cccAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGCGTTCATGGCCAAACCCTTCACTGTGcc > 1:243456/1‑76 (MQ=255)
ccAGCCCGCCAGCCAGGCAATGACTTGCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGccc < 2:1225307/76‑1 (MQ=255)
cAGCCCGCCAGCCAGGCAGAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCa < 2:345312/76‑1 (MQ=255)
cAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCa > 2:1871962/1‑76 (MQ=255)
aGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCTCGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCAt < 1:1835943/76‑1 (MQ=255)
cccGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAg < 1:400481/76‑1 (MQ=255)
agGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAAt > 2:50115/1‑76 (MQ=255)
gCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGccn < 1:1046485/60‑2 (MQ=255)
gCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGccc > 2:1046485/1‑60 (MQ=255)
cAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGAc > 2:1635354/1‑76 (MQ=255)
aaaGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGAcc < 2:1864707/76‑1 (MQ=255)
aaaGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGAcc > 2:397986/1‑76 (MQ=255)
aaaGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGAcc > 2:762036/1‑76 (MQ=255)
aaaGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGAcc > 1:1217917/1‑76 (MQ=255)
aaGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCg > 1:828630/1‑76 (MQ=255)
aCTTTCGGACCGAAGTCACGAGCTGGGTTCAAGGCCAAAACCGTCAGTGGATTAATAGATGCGCCAATGATAGCaa > 2:359967/1‑76 (MQ=255)
cTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAAt < 2:789263/76‑1 (MQ=255)
tttCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc < 1:981927/76‑1 (MQ=255)
tttCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc < 1:1241391/76‑1 (MQ=255)
tttCGGACCGAAATCGCGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc < 2:1900369/76‑1 (MQ=255)
tttCGGACCCAAGACTCGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc < 1:476876/76‑1 (MQ=255)
tttCGGAACGAAGTTACGCGCTGGGTTCAAGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc < 1:1010604/76‑1 (MQ=255)
tttCGAACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATc < 2:1665662/76‑1 (MQ=255)
ttCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCa < 1:1702764/76‑1 (MQ=255)
tCGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAg > 1:1693659/1‑76 (MQ=255)
cGGACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGt > 1:249774/1‑76 (MQ=255)
ggACCGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTa > 2:1471699/1‑76 (MQ=255)
ccGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAc < 1:504597/76‑1 (MQ=255)
ccGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAc < 2:1126938/76‑1 (MQ=255)
cGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCTAATGACCGCAATCAGTAGAcc < 2:1850442/76‑1 (MQ=255)
cGAAGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGAcc > 1:541818/1‑76 (MQ=255)
aaGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCaa < 2:1041565/76‑1 (MQ=255)
aaGTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGAGGCGCCAATGACCGCAATCAGTAGACCaa < 2:1225568/76‑1 (MQ=255)
gTCACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATc < 2:1282722/76‑1 (MQ=255)
cACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAg < 2:248600/76‑1 (MQ=255)
cACGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAg < 2:541818/76‑1 (MQ=255)
aCGCGCTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGc < 1:1741300/76‑1 (MQ=255)
gcTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAgg > 2:957509/1‑76 (MQ=255)
gcTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAgg > 1:385017/1‑76 (MQ=255)
gcTGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAgg < 1:1165399/76‑1 (MQ=255)
tGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGa > 2:1433649/1‑76 (MQ=255)
tGGGTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGa > 2:1204577/1‑76 (MQ=255)
gggTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAg > 2:1859823/1‑76 (MQ=255)
ggTTCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGc > 2:854599/1‑76 (MQ=255)
ttCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCa < 2:635507/76‑1 (MQ=255)
tCATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCaa < 1:624318/76‑1 (MQ=255)
cacggCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCaaa > 2:1385295/4‑76 (MQ=255)
cATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCaaa > 2:1858059/1‑76 (MQ=255)
cATGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCaaa > 2:645071/1‑76 (MQ=255)
tGGCCAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAgg > 2:891422/1‑76 (MQ=255)
|
TAAAGGCGACATTGCCCCAGCCCGCCAGCCAGGCAAAGACTTTCGGACCGAAGTCACGCGCTGGGTTCATGGCAAAACCCGTCAATGGGCCCATAGATGCGCCAATGACCGCAATCAGTAGACCAATCAGCAAGGGAGCCAAAGG > REL606/4096900‑4097044
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
