Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A114 F1 I1 R1
|
20 |
239.5 |
15463816 |
93.1% |
14396812 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,273,299 |
A→T |
intergenic (+227/‑115) |
nrfG → / → gltP |
heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,273,299 | 0 | A | T | 98.1%
| 1107.5
/ 7.1
| 267 | intergenic (+227/‑115) | nrfG/gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| Reads supporting (aligned to +/- strand): ref base A (3/2); new base T (142/120); total (145/122) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 |
Only 100 of 267 total aligned reads displayed.
CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371
|
cgTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTc < 2:6474698/76‑1 (MQ=255)
tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa > 1:2536908/1‑76 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATac > 2:1573460/1‑75 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:3529461/1‑76 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:5442525/76‑1 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:4671368/76‑1 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg > 2:3968280/1‑76 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 1:5394696/76‑1 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 1:2755129/76‑1 (MQ=255)
tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc < 2:6316155/76‑1 (MQ=255)
gCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGtt > 1:1038385/1‑76 (MQ=255)
gCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGtt < 2:5572891/76‑1 (MQ=255)
aTTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaa > 1:2588692/1‑76 (MQ=255)
ttAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaa < 1:5703778/76‑1 (MQ=255)
ttAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaa > 1:1673538/1‑76 (MQ=255)
ttAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaa < 1:1350269/76‑1 (MQ=255)
tAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaa < 2:1333649/76‑1 (MQ=255)
tAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaa > 1:370379/1‑76 (MQ=255)
aaTGCGGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa < 1:2027884/76‑1 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 2:5979755/1‑76 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 2:5374520/1‑76 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 2:3598254/1‑76 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 1:3467496/1‑76 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa < 2:2138788/76‑1 (MQ=255)
aTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaa < 2:2349592/76‑1 (MQ=255)
tGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaaa > 2:5178797/1‑76 (MQ=255)
gCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAAt > 2:2879351/1‑76 (MQ=255)
cAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATc < 1:3144341/76‑1 (MQ=255)
aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt < 2:6705442/76‑1 (MQ=255)
cGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTAc < 1:7630764/76‑1 (MQ=255)
gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa > 1:1531840/1‑76 (MQ=255)
gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACa < 1:6131688/76‑1 (MQ=255)
aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaa > 2:480969/1‑76 (MQ=255)
aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaa > 2:5073743/1‑76 (MQ=255)
aaaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaa < 1:2881829/76‑1 (MQ=255)
aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaaa < 1:5730075/76‑1 (MQ=255)
aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACAGTTCATAAGCGTTAAAAAAATCTACaaa > 2:3326035/1‑76 (MQ=255)
aGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAcc > 1:4592499/1‑76 (MQ=255)
aGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAcc < 1:7469232/76‑1 (MQ=255)
gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCa < 2:5815453/76‑1 (MQ=255)
tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAc > 1:5877760/1‑76 (MQ=255)
tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAc > 2:7071399/1‑76 (MQ=255)
ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACg > 1:4677070/1‑76 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 2:4242881/1‑76 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa < 1:4966099/76‑1 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 2:6135822/1‑76 (MQ=255)
ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa < 1:7232541/76‑1 (MQ=255)
ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa > 1:7667772/1‑76 (MQ=255)
gttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAaca < 1:1545226/76‑1 (MQ=255)
ttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAacac > 1:446874/1‑76 (MQ=255)
tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAAcaca < 1:2169708/76‑1 (MQ=255)
tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAAcaca < 1:3342592/76‑1 (MQ=255)
tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAAGCAAcaca > 2:6991262/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:6274097/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 1:4146363/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:1697302/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:7425203/1‑76 (MQ=255)
aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt > 1:5176210/1‑76 (MQ=255)
aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt > 2:6015103/1‑76 (MQ=255)
aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt < 2:5100118/76‑1 (MQ=255)
gggtGCGCAACATACCGCGCACATGATACTGTTGATAAGCGTTAAAAAAATATAGTCACCAACGTACCACACTTCa > 2:708514/4‑76 (MQ=255)
ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 1:1298779/1‑76 (MQ=255)
ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 1:3429880/1‑76 (MQ=255)
ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 1:3874633/1‑76 (MQ=255)
ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa < 1:6589134/76‑1 (MQ=255)
ggtGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAt < 2:1934797/74‑1 (MQ=255)
gTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCAt < 2:815165/76‑1 (MQ=255)
tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc > 2:3653862/1‑76 (MQ=255)
gcgcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGcc > 2:3546306/1‑76 (MQ=255)
gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt < 1:7178884/76‑1 (MQ=255)
gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt > 2:4532714/1‑76 (MQ=255)
gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt > 1:4763371/1‑76 (MQ=255)
gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt < 1:1903151/76‑1 (MQ=255)
cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg < 2:1151681/76‑1 (MQ=255)
cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg < 1:5541117/76‑1 (MQ=255)
cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg > 1:3218617/1‑76 (MQ=255)
aaCATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTgg < 2:4950356/76‑1 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:7564113/1‑76 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:7288860/1‑76 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:864020/1‑76 (MQ=255)
cATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 2:4572754/1‑76 (MQ=255)
aTACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAg > 1:84770/1‑76 (MQ=255)
aCCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 1:1145404/1‑76 (MQ=255)
aCCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 1:2816949/1‑76 (MQ=255)
ccGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTAt < 2:5617845/76‑1 (MQ=255)
gcgcAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGt < 1:1163540/76‑1 (MQ=255)
cgcAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTc < 1:693397/76‑1 (MQ=255)
gcAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCa > 2:4824448/1‑76 (MQ=255)
aaTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt > 1:1453278/1‑76 (MQ=255)
aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt < 1:623272/76‑1 (MQ=255)
aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt < 1:3669492/76‑1 (MQ=255)
gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 2:1222954/1‑76 (MQ=255)
gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:6621163/1‑76 (MQ=255)
gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct > 1:4276357/1‑76 (MQ=255)
aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 1:6882804/1‑76 (MQ=255)
aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 2:5505766/1‑76 (MQ=255)
tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg < 2:6907588/76‑1 (MQ=255)
tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg > 2:2636840/1‑76 (MQ=255)
tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg > 2:4196410/1‑76 (MQ=255)
aCTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgc > 1:6506795/1‑76 (MQ=255)
|
CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
