Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A68 F1 I1 R1
|
17 |
56.4 |
3914180 |
96.2% |
3765441 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,273,299 |
A→T |
intergenic (+227/‑115) |
nrfG → / → gltP |
heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,273,299 | 0 | A | T | 97.7%
| 509.3
/ 0.8
| 129 | intergenic (+227/‑115) | nrfG/gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (71/55); total (73/56) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 |
Only 100 of 129 total aligned reads displayed.
CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371
|
cgTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTc > 2:1715190/1‑76 (MQ=255)
ttattaTGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCAt < 1:1107961/76‑1 (MQ=255)
tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa > 1:1775858/1‑76 (MQ=255)
tattatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATa > 1:283669/1‑76 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:148691/1‑76 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:1238805/1‑76 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:1943023/1‑76 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATaa > 1:218402/1‑76 (MQ=255)
attatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATaa > 1:1808491/1‑76 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 2:1717289/76‑1 (MQ=255)
ttatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAg < 1:1446729/76‑1 (MQ=255)
tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc < 2:710142/76‑1 (MQ=255)
tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc < 1:724457/76‑1 (MQ=255)
tatGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGc > 1:1291838/1‑76 (MQ=255)
tGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGt > 1:250039/1‑76 (MQ=255)
tGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGt < 2:319393/76‑1 (MQ=255)
gCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGtt < 1:191019/76‑1 (MQ=255)
aTTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaa < 2:1173471/76‑1 (MQ=255)
aTTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaa < 2:219834/76‑1 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa < 2:893982/76‑1 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 1:1645111/1‑76 (MQ=255)
aaTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaa > 1:1445141/1‑76 (MQ=255)
aTGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaa > 2:1555317/1‑76 (MQ=255)
tGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTaaaaaaa < 2:1437841/76‑1 (MQ=255)
cAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATc > 1:639849/1‑76 (MQ=255)
aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt < 2:538300/76‑1 (MQ=255)
aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt < 1:395786/76‑1 (MQ=255)
aGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCt > 1:1144137/1‑76 (MQ=255)
gCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTa < 2:1400366/76‑1 (MQ=255)
gCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTa < 2:1936010/76‑1 (MQ=255)
gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTAc > 1:1544588/1‑75 (MQ=255)
gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTAc > 1:332258/1‑75 (MQ=255)
gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTAc < 2:1544588/75‑1 (MQ=255)
gAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTAc < 2:332258/75‑1 (MQ=255)
aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACaaa > 1:852008/1‑76 (MQ=255)
aaaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACaaa > 1:524825/1‑76 (MQ=255)
aaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAc < 2:240725/76‑1 (MQ=255)
aaGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAc > 2:125523/1‑76 (MQ=255)
aGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAAcc < 1:1754588/76‑1 (MQ=255)
gCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCa < 1:922131/76‑1 (MQ=255)
ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCaa > 2:38522/1‑76 (MQ=255)
ctctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCaa > 2:1254192/1‑76 (MQ=255)
tctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAAc > 2:1333525/1‑76 (MQ=255)
ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACg > 1:1172449/1‑76 (MQ=255)
ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACg > 1:777950/1‑76 (MQ=255)
ctGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACg < 1:1380298/76‑1 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa < 2:1334934/76‑1 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa < 2:1692640/76‑1 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 2:1497747/1‑76 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 2:406035/1‑76 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa < 2:585718/76‑1 (MQ=255)
gttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCa > 1:1543271/1‑76 (MQ=255)
ttgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCaa > 1:1263765/1‑76 (MQ=255)
tgttAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAac > 1:631451/1‑76 (MQ=255)
tAAAGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAAcaca > 1:1455015/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:241122/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:426586/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 1:442319/1‑76 (MQ=255)
aaaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACaa > 2:784481/1‑76 (MQ=255)
aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt > 2:338772/1‑76 (MQ=255)
aaGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAt > 2:1711237/1‑76 (MQ=255)
aGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAtt > 2:1354723/1‑76 (MQ=255)
aGGGTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAAtt < 2:503967/76‑1 (MQ=255)
ggTTGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCa > 2:1195983/1‑76 (MQ=255)
ttGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATg > 2:1409668/1‑76 (MQ=255)
ttGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATg > 1:574040/1‑76 (MQ=255)
tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc < 1:1100454/76‑1 (MQ=255)
tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc < 1:1100467/76‑1 (MQ=255)
tGCGCAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc > 2:1951602/1‑76 (MQ=255)
tGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGc < 2:42389/76‑1 (MQ=255)
gcgcAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGcc < 1:979304/76‑1 (MQ=255)
gcAACATACCGCGCAAATGATACTGTTCATAAGCGTTGAAAAAATCTACAAACCAACGCAACACAATTCATGCCCt > 2:1800174/1‑76 (MQ=255)
cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg > 2:24598/1‑76 (MQ=255)
cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg < 2:1905306/76‑1 (MQ=255)
cAACATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTg < 2:1419980/76‑1 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa < 1:1366458/76‑1 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 1:1371789/1‑76 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 1:1122895/1‑76 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa < 2:668506/76‑1 (MQ=255)
cATACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCa > 1:1168471/1‑76 (MQ=255)
aTACCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAg > 1:1557584/1‑76 (MQ=255)
aCCGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTa > 2:178140/1‑76 (MQ=255)
ccGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTAt < 2:1280074/76‑1 (MQ=255)
ccGCGCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTAt < 2:1280046/76‑1 (MQ=255)
cgcgCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 1:1765386/76‑1 (MQ=255)
cgcgCAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATg < 1:1316691/76‑1 (MQ=255)
gcAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCa < 2:165470/76‑1 (MQ=255)
cAAATGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCAc < 2:495721/76‑1 (MQ=255)
aaaTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACg > 1:893421/1‑76 (MQ=255)
aaTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt > 1:680472/1‑76 (MQ=255)
aaTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGt > 1:486755/1‑76 (MQ=255)
aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt > 1:195330/1‑76 (MQ=255)
aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt < 1:632633/76‑1 (MQ=255)
aTGATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGtt > 1:681218/1‑76 (MQ=255)
gATACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtct < 2:854221/76‑1 (MQ=255)
aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc > 2:1480950/1‑76 (MQ=255)
aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc < 2:1578591/76‑1 (MQ=255)
aTACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTtctc < 2:186686/76‑1 (MQ=255)
tACTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcg > 2:353812/1‑76 (MQ=255)
cTGTTCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTcgcg > 1:736273/1‑76 (MQ=255)
|
CGTTATTATGCATTAATGCAGCGAAAAGCTCTGTTGTTAAAGGGTTGCGCAACATACCGCGCAAATGATACTGATCATAAGCGTTAAAAAAATCTACAAACCAACGCAACACAATTCATGCCCTGGCAGTATGTCACGTTCTCGCG > REL606/4273226‑4273371
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
