mutation predictions | marginal predictions | summary statistics | genome diff | command line log
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breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | |||||
|---|---|---|---|---|---|
| evidence | position | mutation | annotation | gene | description |
| RA | 645,855 | A→G | L231S (TTA→TCA) | dacA ← | D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5) |
| RA | 1,305,693 | C→A | G183W (GGG→TGG) | cls ← | cardiolipin synthetase |
| RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
| JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
| JC JC | 1,821,482 | IS150 (+) +3 bp | coding (616‑618/1041 nt) | ynjI ← | predicted inner membrane protein |
| MC JC | 2,100,308 | Δ22,146 bp | ogrK–[ECB_02013] | 27 genes ogrK, yegZ, ECB_01989, ECB_01990, ECB_01991, ECB_01992, ECB_01993, ECB_01994, ECB_01995, ECB_01996, ECB_01997, ECB_01998, ECB_01999, ECB_02000, ECB_02001, ECB_02002, ECB_02003, ECB_02004, ECB_02005, ECB_02006, ECB_02007, ECB_02008, ECB_02009, ECB_02010, ECB_02011, ECB_02012, [ECB_02013] |
|
| RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
| RA | 2,762,214 | A→G | Y67H (TAT→CAT) | rpoS ← | RNA polymerase sigma factor |
| RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
| RA | 3,881,536 | T→G | D7A (GAT→GCT) | atpE ← | F0F1 ATP synthase subunit C |
| MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
| RA | 3,911,997 | (A)6→7 | intergenic (‑198/‑125) | yifB ← / → ilvL | predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide |
| RA | 4,167,434 | T→C | V825A (GTT→GCT) | rpoC → | DNA‑directed RNA polymerase subunit beta' |
| RA | 4,273,299 | A→T | intergenic (+227/‑115) | nrfG → / → gltP | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/glutamate/aspartate:proton symporter |
| RA | 4,442,003 | C→A | A265E (GCG→GAG) | pmbA → | predicted peptidase required for the maturation and secretion of the antibiotic peptide MccB17 |
| JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
| Unassigned missing coverage evidence | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | REL606 | 546984–547700 | 593362 | 45663–46379 | 18 [14] | [17] 18 | [insB‑6]–[fepA] | [insB‑6],insA‑6,nmpC,ybcR,ybcS,ybcT,ybcU,ECB_00510,nohB,ECB_00512,ECB_00513,ECB_00514,ECB_00515,ECB_00516,ECB_00517,appY,ompT,envY,ybcH,nfrA,nfrB,nfrB,ECB_00524,yhhI,ECB_00526,ECB_00527,ECB_00528,ECB_00529,ECB_00530,cusS,cusR,cusC,ylcC,cusB,cusA,pheP,ybdG,nfnB,ybdF,ybdJ,ybdK,insJ‑1,insK‑1,insB‑7,insA‑7,insK‑1,hokE,insL‑3,entD,[fepA] |
| * | * | ÷ | REL606 | 593574 | 619833–619116 | 25543–26260 | 19 [16] | [17] 18 | [fepA]–insA‑8 | [fepA],fes,ybdZ,entF,fepE,fepC,fepG,fepD,ybdA,fepB,entC,entE,entB,entA,ybdB,cstA,cstA,ybdD,ybdH,ybdL,ybdM,ybdN,insB‑8,insA‑8 |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | REL606 | 2199003 = | 6 (0.100) | 64 (1.050) | 48/150 | 0.2 | 91.4% | coding (768/1470 nt) | lysP | lysine transporter |
| ? | REL606 | = 2199011 | NA (NA) | coding (760/1470 nt) | lysP | lysine transporter | |||||