Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A59 F1 I1 R1	21	55.8	3542164	96.7%	3425272	76.0

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	REL606	1,609,130	A→C	K285Q (AAG→CAG)	insF‑3 →	putative transposase for IS3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	REL606	1,609,130	0	A	C	100.0%	78.5 / NA	20	K285Q (AAG→CAG)	insF‑3	putative transposase for IS3
Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (7/13); total (7/13)

GGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGT  >  REL606/1609103‑1609201

ggcCTCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTc                         >  2:1243693/1‑76 (MQ=14)

    tCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCt                     >  2:358623/1‑76 (MQ=255)

    tCAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCt                     <  2:1166178/76‑1 (MQ=255)

     cAGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTa                    <  1:417585/76‑1 (MQ=255)

      aGTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTaa                   <  2:1400654/76‑1 (MQ=255)

       gTCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTaaa                  <  1:326635/76‑1 (MQ=255)

        tCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAAt                 >  2:552048/1‑76 (MQ=255)

        tCCGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAAt                 <  2:465118/76‑1 (MQ=255)

         ccGGAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATa                <  1:1382921/76‑1 (MQ=255)

           ggAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACg              <  1:1562354/76‑1 (MQ=255)

            gAACAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGa             <  1:235811/76‑1 (MQ=255)

               cAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGc          <  2:1746694/76‑1 (MQ=255)

               cAATTTGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGc          <  2:760515/76‑1 (MQ=255)

                    tGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGc     >  2:1318888/1‑76 (MQ=255)

                    tGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGc     >  2:1464407/1‑76 (MQ=255)

                    tGAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGc     >  2:757470/1‑76 (MQ=255)

                     gAAAACCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCt    <  1:171670/76‑1 (MQ=255)

                      aaaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTg   >  2:1004684/1‑76 (MQ=255)

                      aaaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTg   <  2:164748/76‑1 (MQ=255)

                       aaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGt  <  2:1092125/76‑1 (MQ=255)

                       aaaCCAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGt  <  1:768558/76‑1 (MQ=255)

GGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATTCACCTAAATACGAGGCATTGCTGT  >  REL606/1609103‑1609201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

N/A