Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A38 F1 I1 R1
|
17 |
54.1 |
3391312 |
97.1% |
3292963 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,496,783 |
C→T |
G418D (GGT→GAT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,496,783 | 0 | C | T | 100.0%
| 168.5
/ NA
| 43 | G418D (GGT→GAT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/15); total (28/15) |
AGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTT > REL606/3496710‑3496855
|
agagTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCg > 2:717485/1‑76 (MQ=255)
agTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGtt > 2:1394376/1‑76 (MQ=255)
ttCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATtca > 1:764040/1‑76 (MQ=255)
ttCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATtca > 1:1559980/1‑76 (MQ=255)
tCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATtcat < 2:1229434/76‑1 (MQ=255)
cGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATtcatca > 1:52434/1‑76 (MQ=255)
acCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATc > 2:1563211/1‑76 (MQ=255)
acCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATc > 2:990413/1‑76 (MQ=255)
ccGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCg < 1:1356105/76‑1 (MQ=255)
ccGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCg > 2:1204210/1‑76 (MQ=255)
ccGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCg < 2:2913/76‑1 (MQ=255)
ccGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCg > 1:9764/1‑76 (MQ=255)
cGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCTATACGCACACGACGATCGTTGGATTCATCAATGATCGa < 2:564035/76‑1 (MQ=255)
cGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGa < 1:321141/76‑1 (MQ=255)
cGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGa > 1:682552/1‑76 (MQ=255)
cGTTAACTTGGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGa < 2:767870/76‑1 (MQ=255)
gTTAACTTTGTGGCCCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGAc > 2:143858/1‑76 (MQ=255)
tttGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGt > 1:197033/1‑76 (MQ=255)
tttGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGt > 2:327500/1‑76 (MQ=255)
tttGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGt > 1:159994/1‑76 (MQ=255)
tgtgGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTcc > 2:1077055/1‑76 (MQ=255)
gCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCcagc > 2:176462/1‑76 (MQ=255)
cTCACAACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCcagca < 2:1112756/76‑1 (MQ=255)
cacaACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGa > 1:1092346/1‑76 (MQ=255)
cacaACAACCGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGa > 2:97983/1‑76 (MQ=255)
cacccGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGt < 2:210070/73‑1 (MQ=255)
ccGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATc > 2:688071/1‑76 (MQ=255)
cGCCAGCCAGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCg > 1:84350/1‑76 (MQ=255)
gccagccaGGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGt < 1:802613/76‑1 (MQ=255)
ccagGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGCTCGGTATCTTTCGGa > 2:132442/1‑76 (MQ=255)
ccagGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGa > 2:132447/1‑76 (MQ=255)
cagGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGAt > 2:1550472/1‑76 (MQ=255)
cagGCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGAt > 2:1161330/1‑76 (MQ=255)
gCCATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACt < 2:1477662/76‑1 (MQ=255)
cATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGt > 2:632591/1‑76 (MQ=255)
cATACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGt > 1:875239/1‑76 (MQ=255)
aTACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGtt < 1:1431966/76‑1 (MQ=255)
aTACGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGtt > 2:525057/1‑76 (MQ=255)
cGCACACGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCt < 1:92459/76‑1 (MQ=255)
cacaCGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGc < 1:273775/76‑1 (MQ=255)
cacaCGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGc > 2:1659818/1‑76 (MQ=255)
cacaCGACGATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGc < 2:1358283/76‑1 (MQ=255)
acgacgATCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAg < 2:1685746/76‑1 (MQ=255)
gacgatCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGt < 1:1310448/76‑1 (MQ=255)
gacgatCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGt > 2:1586317/1‑76 (MQ=255)
cgatCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGttt < 1:938925/76‑1 (MQ=255)
cgacCGTTGGATTCATCAAGGGTCGACGCCCGTCCCAGCAGATCGGTATCGTTCAAATACTGTTCCTGCATGGttt < 2:1307691/76‑1 (MQ=255)
|
AGAGTGCAGTTCCGATACACCGTTAACTTTGTGGCTCACAACAACCGCCAGCCAGGCCATACGCACACGACGACCGTTGGATTCATCAATGATCGACGCCCGTCCCAGCAGATCGGTATCGTTCGGATACTGTTCCTGCAAGGTTT > REL606/3496710‑3496855
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
