Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A41 F1 I1 R1
|
21 |
57.1 |
3643130 |
97.0% |
3533836 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,497,167 |
A→G |
V290A (GTT→GCT) |
glgP ← |
glycogen phosphorylase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,497,167 | 0 | A | G | 100.0%
| 161.3
/ NA
| 41 | V290A (GTT→GCT) | glgP | glycogen phosphorylase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (24/17); total (24/17) |
TTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCG > REL606/3497094‑3497237
|
tttATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGcc > 2:1366414/1‑76 (MQ=255)
ttATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGGGGAAGCCa > 1:1245814/1‑76 (MQ=255)
aTCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAgg > 1:1110305/1‑76 (MQ=255)
aTCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAgg < 2:841399/76‑1 (MQ=255)
cGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTACAATGTCCTGCATGGTCGAGGAAGCCAGGAAg > 1:547872/1‑76 (MQ=255)
cGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAg < 2:1027882/76‑1 (MQ=255)
cAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTAt < 1:786631/76‑1 (MQ=255)
cAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTAt > 2:1538237/1‑76 (MQ=255)
aGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTTAAATGTCCTGAATGGGTGGGGGAGCCCGGGAGTAtt > 1:193853/1‑76 (MQ=255)
ggTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTc < 2:1724683/76‑1 (MQ=255)
gTTATCGTAGGTTTTATGCAACTGATAATGGCGGTTTAAAATTTCCTGAATGGCCGAGGGAGCCAGGAAGTTTTct > 1:1770547/1‑75 (MQ=255)
ttATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGGATTCCt > 1:601770/1‑76 (MQ=255)
tATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTg < 2:1451508/76‑1 (MQ=255)
tATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTg < 2:343773/76‑1 (MQ=255)
aTCGTAGGTTTTATGCAACTGATACTGGCGGCTTAAAATGGTCTGAATGGTCGAGGGAGCCCGGAAGTATTCCTGa > 1:694924/1‑76 (MQ=255)
aTCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGa > 1:700805/1‑76 (MQ=255)
gTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGc > 2:46993/1‑76 (MQ=255)
tAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCa > 2:1591856/1‑76 (MQ=255)
aGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAg > 2:1722220/1‑76 (MQ=255)
aGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAg < 1:1191229/76‑1 (MQ=255)
gTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTAATCCTTacgcacgc > 2:908603/1‑76 (MQ=255)
ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGCGGAAGGCCGGAAGGAGTCCGGGCGCAGgcgcc > 1:216413/1‑75 (MQ=255)
ttATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCa < 1:1521204/76‑1 (MQ=255)
aaCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCAc > 2:898260/1‑76 (MQ=255)
aaCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCAc < 2:717557/76‑1 (MQ=255)
aaCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCAc > 2:1548136/1‑76 (MQ=255)
aCTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACg < 1:1112234/76‑1 (MQ=255)
gATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGGGCAGCTCACGccc > 1:66865/1‑76 (MQ=255)
gATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGccc < 1:1642585/76‑1 (MQ=255)
aTAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACccccc > 2:492711/1‑75 (MQ=255)
tAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCgg < 2:860693/76‑1 (MQ=255)
tGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATCCCTGACGCAGGCGCAGCTCACGCCCGGAGt > 1:1485574/1‑76 (MQ=255)
ggcggcTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTa < 2:478392/76‑1 (MQ=255)
gcggcTTAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAg > 2:688650/1‑76 (MQ=255)
tAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAg > 1:1005572/1‑76 (MQ=255)
tAAAATGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAg < 1:35854/76‑1 (MQ=255)
aaaaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGt > 2:189149/1‑76 (MQ=255)
aaaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTc > 2:225809/1‑76 (MQ=255)
aaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCa < 2:1689420/76‑1 (MQ=255)
aaTGTCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCa > 2:358570/1‑76 (MQ=255)
tCCTGAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCg > 1:397576/1‑76 (MQ=255)
gAATGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATa > 1:701241/1‑76 (MQ=255)
aTGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACa > 1:365561/1‑76 (MQ=255)
tGGTCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAg > 2:1748347/1‑76 (MQ=255)
tCGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAc > 1:977018/1‑76 (MQ=255)
cGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcg > 2:1136916/1‑76 (MQ=255)
cGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcg < 1:615552/76‑1 (MQ=255)
cGAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcg < 1:928044/76‑1 (MQ=255)
gAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgc > 2:355856/1‑76 (MQ=255)
gAGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGAGGAGTCATCCGGATACAGTAcgc > 2:229121/1‑76 (MQ=255)
gAGGAAGCCAGGAAGTATTCCTGACGCAGGCGAAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgc > 2:736032/1‑76 (MQ=255)
aGGAAGCCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTAcgcg > 1:1362878/1‑76 (MQ=255)
|
TTTATCCGCCAGGTTATCGTAGGTTTTATGCAACTGATAATGGCGGCTTAAAATGTCCTGAATGGTCGAGGAAACCAGGAAGTATTCCTGACGCAGGCGCAGCTCACGCCCGGAGTAGGTGGAGTCATCCGGATACAGTACGCG > REL606/3497094‑3497237
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
