Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A64 F1 I1 R1
|
17 |
58.5 |
3743480 |
96.8% |
3623688 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,163,749 |
A→C |
Q965P (CAG→CCG) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,163,749 | 0 | A | C | 100.0%
| 179.5
/ NA
| 43 | Q965P (CAG→CCG) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (27/16); total (27/16) |
CAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCT > REL606/4163681‑4163821
|
cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTc > 2:455094/1‑76 (MQ=255)
cAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTc > 1:391373/1‑76 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg < 1:1475333/76‑1 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg < 1:997734/76‑1 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg > 1:1675662/1‑76 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg > 1:6374/1‑76 (MQ=255)
aaaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCg < 2:20876/76‑1 (MQ=255)
aaCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGa > 1:715231/1‑76 (MQ=255)
aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGaa > 2:882680/1‑76 (MQ=255)
aCGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGaa < 2:1290579/76‑1 (MQ=255)
gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGc > 1:306708/1‑76 (MQ=255)
gTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGc > 2:650845/1‑76 (MQ=255)
cgcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCggg > 1:895306/1‑76 (MQ=255)
gcTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGt < 1:1506022/76‑1 (MQ=255)
tGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCt < 2:768905/76‑1 (MQ=255)
gAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGt > 2:427062/1‑76 (MQ=255)
aaaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGACCTGCCGATCCTCGAAGCGGGTCTGtt < 1:614546/76‑1 (MQ=255)
aaTCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTc < 2:954226/76‑1 (MQ=255)
gaaATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTa > 2:1496450/1‑76 (MQ=255)
gCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgct > 1:1634720/1‑76 (MQ=255)
cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg > 2:1013660/1‑76 (MQ=255)
cAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtg > 2:1685515/1‑76 (MQ=255)
aaaCAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCgtgctgtgc > 2:1283847/1‑76 (MQ=255)
aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGtgctgtgctg < 1:1716465/76‑1 (MQ=255)
aCAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGtgctgtgctg < 1:427062/76‑1 (MQ=255)
cAGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTgg < 2:1544402/76‑1 (MQ=255)
aGGCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGt > 2:1584801/1‑76 (MQ=255)
gCGAAGAAAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAg < 2:1453612/76‑1 (MQ=255)
gaagaaAGACCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGcc < 1:1762646/76‑1 (MQ=255)
aagaaagcCCGGTCTGAAGAACTCCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg < 2:1384174/76‑1 (MQ=255)
aagaaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg > 1:1104836/1‑76 (MQ=255)
aagaaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCg > 1:250336/1‑76 (MQ=255)
aagaaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGGTCAGCCGTATCCGTGCTGTGCTGGTAGCCg > 1:844498/1‑76 (MQ=255)
aaagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTg > 2:1210927/1‑76 (MQ=255)
aagaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTgg > 2:1052710/1‑76 (MQ=255)
agaCCTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGc < 1:594637/76‑1 (MQ=255)
cTGTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTg > 2:95354/1‑76 (MQ=255)
gTCTGAAGAACTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa > 2:1177969/1‑76 (MQ=255)
gTCTGAAAACCGGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGaa < 2:929443/76‑1 (MQ=255)
aagaaCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga > 2:1611538/1‑76 (MQ=255)
aagaaCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTga > 2:462608/1‑76 (MQ=255)
gatcTGCGGATCCGCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTgaga < 1:680733/73‑1 (MQ=255)
atcTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa < 2:755379/74‑1 (MQ=255)
aaCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGaa < 2:188643/76‑1 (MQ=255)
aCTGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAg > 1:835620/1‑76 (MQ=255)
tGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGGAGCTGAGAAGCt > 1:1476199/1‑76 (MQ=255)
tGCCGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCt < 2:1780994/76‑1 (MQ=255)
|
CAAACGTGCGCTGGAAATCGAAGAAATGCAGCTCAAACAGGCGAAGAAAGACCTGTCTGAAGAACTGCAGATCCTCGAAGCGGGTCTGTTCAGCCGTATCCGTGCTGTGCTGGTAGCCGGTGGCGTTGAAGCTGAGAAGCT > REL606/4163681‑4163821
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
