Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A108 F1 I1 R1
|
80 |
195.1 |
12639756 |
93.3% |
11792892 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,076,609 |
G→A |
T46T (ACG→ACA) |
yegE → |
predicted diguanylate cyclase, GGDEF domain signalling protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,076,609 | 0 | G | A | 99.4%
| 649.0
/ ‑4.0
| 162 | T46T (ACG→ACA) | yegE | predicted diguanylate cyclase, GGDEF domain signalling protein |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (85/76); total (85/77) |
| Fisher's exact test for biased strand distribution p-value = 4.75e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.79e-01 |
Only 100 of 168 total aligned reads displayed.
TAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTG > REL606/2076536‑2076681
|
taggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATc > 2:2784400/1‑76 (MQ=255)
taggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATc < 2:1553961/76‑1 (MQ=255)
taggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATc > 2:5321742/1‑76 (MQ=255)
aggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATcc < 1:3210090/76‑1 (MQ=255)
ggtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCca > 1:5158677/1‑76 (MQ=255)
gtttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCcat < 2:3220596/76‑1 (MQ=255)
tttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGAcatccatc > 2:2945990/1‑76 (MQ=255)
tttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGAcatccatc > 1:1481337/1‑76 (MQ=255)
ttagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCcatca > 1:1613180/1‑76 (MQ=255)
tagTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCcatcat < 2:2459043/76‑1 (MQ=255)
agTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCTGACATCCATCatg > 2:3986024/1‑76 (MQ=255)
agTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatg > 2:5574831/1‑76 (MQ=255)
gTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatga < 1:4300783/76‑1 (MQ=255)
tctcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatgat < 1:6105780/76‑1 (MQ=255)
ctcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCatgatg > 2:6055522/1‑76 (MQ=255)
tcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtgg > 1:5675713/1‑76 (MQ=255)
tcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtgg < 2:101968/76‑1 (MQ=255)
tcGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCACGACATCCATCATGAtgg > 2:50426/1‑76 (MQ=255)
cGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggt > 1:3386565/1‑76 (MQ=255)
cGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggt > 2:4847141/1‑76 (MQ=255)
gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtg < 2:3856759/76‑1 (MQ=255)
gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtg < 1:1799985/76‑1 (MQ=255)
gTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtg < 2:4417025/76‑1 (MQ=255)
tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtgg > 2:5984407/1‑76 (MQ=255)
tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtgg > 1:2546975/1‑76 (MQ=255)
tttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGAtggtgg < 2:6300756/76‑1 (MQ=255)
ttATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGc < 1:1768329/76‑1 (MQ=255)
tATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCg < 1:2237466/76‑1 (MQ=255)
tCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGtt > 2:5668369/1‑76 (MQ=255)
cTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGttt > 2:5364868/1‑76 (MQ=255)
cTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGttt > 2:2105038/1‑76 (MQ=255)
ttACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTa < 2:243550/76‑1 (MQ=255)
tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 1:1009018/1‑76 (MQ=255)
tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 2:2165306/1‑76 (MQ=255)
tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 2:2529383/1‑76 (MQ=255)
tACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTAt > 1:3073366/1‑76 (MQ=255)
aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATc > 1:6007376/1‑76 (MQ=255)
aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATc > 2:135247/1‑76 (MQ=255)
aCCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATc > 1:5309804/1‑76 (MQ=255)
cccTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCg < 1:2147977/76‑1 (MQ=255)
ttttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCa < 2:3097622/76‑1 (MQ=255)
ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg < 2:1837941/76‑1 (MQ=255)
ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg > 2:585096/1‑76 (MQ=255)
ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg > 1:1422924/1‑76 (MQ=255)
ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg < 2:4934617/76‑1 (MQ=255)
ttCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATg > 1:5538726/1‑76 (MQ=255)
tctcGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGCTGGCGTTTTATCGCCATGc > 1:3576116/1‑76 (MQ=255)
tcGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCg > 1:4481275/1‑76 (MQ=255)
tcGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCg > 1:5730503/1‑76 (MQ=255)
cGCTTGGGCTATCGCAGTTTGCCCAACTGTGGTGCCCGACATAGATCTTGATGATGGCGCTTTATCGCCATGTCgg > 2:2514107/1‑76 (MQ=255)
cGCTTGAGCTTTCTGAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCgg < 2:2972062/76‑1 (MQ=255)
cTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgc > 1:5121732/1‑76 (MQ=255)
ttGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgcg > 2:507691/1‑76 (MQ=255)
ttGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgcg > 1:4872291/1‑76 (MQ=255)
ttGAGCTTTCGCAGTTTGCCCCACTGTGATTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGgcg < 2:2700007/76‑1 (MQ=255)
cTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgt > 2:81337/1‑76 (MQ=255)
cTTTCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgt < 1:1925470/76‑1 (MQ=255)
tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg > 2:3650613/1‑76 (MQ=255)
tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg < 2:4977642/76‑1 (MQ=255)
tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg > 1:3950809/1‑76 (MQ=255)
tttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCAtgtg > 2:297747/1‑76 (MQ=255)
ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 2:1895201/76‑1 (MQ=255)
ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 1:1537934/76‑1 (MQ=255)
ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 1:599992/76‑1 (MQ=255)
ttCGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTgg < 2:5791846/76‑1 (MQ=255)
cGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGcc < 1:5848798/76‑1 (MQ=255)
cGCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGcc < 2:2233703/76‑1 (MQ=255)
gCAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCg > 2:3415115/1‑76 (MQ=255)
cAGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCgg > 2:2116019/1‑76 (MQ=255)
aGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCggg < 1:4090420/76‑1 (MQ=255)
aGTTTGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCggg > 2:6213071/1‑76 (MQ=255)
tttGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGaa > 1:2415938/1‑76 (MQ=255)
ttGCCCCACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAAt > 1:6307523/1‑76 (MQ=255)
ccccACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgc < 1:6047080/76‑1 (MQ=255)
cccACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgcg < 1:2732061/76‑1 (MQ=255)
cccACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTgcg > 2:1469820/1‑76 (MQ=255)
cACTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCt < 2:2231890/76‑1 (MQ=255)
tcTGTGGTTCCCGGCATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTg < 2:4934181/75‑1 (MQ=255)
aCTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTg > 1:803897/1‑76 (MQ=255)
aCTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTg < 2:5740493/76‑1 (MQ=255)
cTGTGGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGa > 2:5513721/1‑76 (MQ=255)
tgGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCt > 1:1075043/1‑76 (MQ=255)
tgGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCt < 1:196880/76‑1 (MQ=255)
tgGTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCt < 2:1387081/76‑1 (MQ=255)
ggTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTg < 2:1935310/76‑1 (MQ=255)
ggTTCCCGACATCCATCATGATGGTGGCGTTTTAACGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTg > 2:5214423/1‑76 (MQ=255)
gTTCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGt > 1:4568262/1‑76 (MQ=255)
ttCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGtt < 2:27156/76‑1 (MQ=255)
ttCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGtt < 1:5275436/76‑1 (MQ=255)
tCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTc > 2:4557884/1‑76 (MQ=255)
tCCCGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTc > 1:245921/1‑76 (MQ=255)
cccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCg > 1:2503762/1‑76 (MQ=255)
cccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCg < 2:6064036/76‑1 (MQ=255)
cccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCg > 1:1864299/1‑76 (MQ=255)
ccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGc > 2:3691778/1‑76 (MQ=255)
ccGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGc < 2:6309093/76‑1 (MQ=255)
cGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCt < 1:611309/76‑1 (MQ=255)
cGACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCt < 2:840318/76‑1 (MQ=255)
gACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTg < 1:737830/76‑1 (MQ=255)
gACATCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTg < 1:298673/76‑1 (MQ=255)
|
TAGGTTTAGTCTCGTTTATCTTTACCCTTTTCTCGCTTGAGCTTTCGCAGTTTGCCCCACTGTGGTTCCCGACGTCCATCATGATGGTGGCGTTTTATCGCCATGCCGGGCGCATGTGGCCGGGAATTGCGCTGAGCTGTTCGCTG > REL606/2076536‑2076681
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
