Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A108 F1 I1 R1
|
80 |
195.1 |
12639756 |
93.3% |
11792892 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,173,730 |
G→A |
R103H (CGT→CAT) |
dld → |
D‑lactate dehydrogenase, FAD‑binding, NADH independent |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,173,730 | 0 | G | A | 99.4%
| 622.6
/ ‑3.9
| 157 | R103H (CGT→CAT) | dld | D‑lactate dehydrogenase, FAD‑binding, NADH independent |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (71/85); total (72/85) |
| Fisher's exact test for biased strand distribution p-value = 4.59e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.79e-01 |
Only 100 of 162 total aligned reads displayed.
ACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCT > REL606/2173657‑2173802
|
aCAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtc < 2:5676430/76‑1 (MQ=255)
aCAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtc < 1:3420618/76‑1 (MQ=255)
cAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtct < 1:6285026/76‑1 (MQ=255)
cAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtct < 1:2684045/76‑1 (MQ=255)
aGGCCTGACCGAAGGATCGACGCCAAACGGTCACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtctc > 2:2061792/1‑76 (MQ=255)
aGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCAtctc < 1:1010596/76‑1 (MQ=255)
ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCg > 1:576910/1‑76 (MQ=255)
ggCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCg < 2:4210002/76‑1 (MQ=255)
ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc < 1:4976945/76‑1 (MQ=255)
ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc < 1:3851/76‑1 (MQ=255)
ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc < 1:2660711/76‑1 (MQ=255)
ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc > 2:645414/1‑76 (MQ=255)
ccTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGAc > 2:6112898/1‑76 (MQ=255)
cggACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACa < 1:4227214/74‑1 (MQ=255)
cTGCCCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACa < 2:1968156/76‑1 (MQ=255)
gACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAg < 2:5480110/76‑1 (MQ=255)
gAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGc > 2:1753323/1‑76 (MQ=255)
aaGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCCCCCTGCATCTCGACAAGCTGCa < 2:4456325/76‑1 (MQ=255)
aaGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCa > 2:4426033/1‑76 (MQ=255)
aaGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCa > 2:3460432/1‑76 (MQ=255)
aGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCAc < 1:3782325/76‑1 (MQ=255)
aTCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGtt > 2:3172158/1‑76 (MQ=255)
aTCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGtt > 1:631865/1‑76 (MQ=255)
tCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTc > 2:5934196/1‑76 (MQ=255)
tCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTc > 2:68935/1‑76 (MQ=255)
gACGCCAAACGGTAACGATTATGATCGCGGTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCATGTTCtt > 1:4419205/1‑76 (MQ=255)
cGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTgg < 1:508516/76‑1 (MQ=255)
cGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCACCTCGACAAGCTGCACGTTCTTgg < 2:1265804/76‑1 (MQ=255)
ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACaa > 1:5137827/1‑59 (MQ=255)
ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACaa < 2:5137827/59‑1 (MQ=255)
ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCa > 2:4171390/1‑76 (MQ=255)
ccAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCa < 1:2747458/76‑1 (MQ=255)
cAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCaa > 2:3881937/1‑76 (MQ=255)
cAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCaa > 2:880964/1‑76 (MQ=255)
cAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCaa < 1:2496863/76‑1 (MQ=255)
aaaCGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAg < 2:2090393/76‑1 (MQ=255)
aaaCGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAg > 1:4525349/1‑76 (MQ=255)
aaaCGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAg < 1:451792/76‑1 (MQ=255)
aaCGGTAACGATTATCATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAgg < 1:186885/76‑1 (MQ=255)
cGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGc > 1:4874837/1‑76 (MQ=255)
cGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGc < 1:4043108/76‑1 (MQ=255)
cGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGc > 1:3264689/1‑76 (MQ=255)
ggTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCg < 1:5308228/76‑1 (MQ=255)
gTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGa > 2:5405403/1‑76 (MQ=255)
tAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa > 2:4239998/1‑76 (MQ=255)
tAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa < 2:4981514/76‑1 (MQ=255)
tAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGaa > 2:1341646/1‑76 (MQ=255)
aaCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAc < 1:4827010/76‑1 (MQ=255)
aaCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAc < 1:2233621/76‑1 (MQ=255)
aaCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAc < 1:2105691/76‑1 (MQ=255)
aCGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACa < 2:3744888/76‑1 (MQ=255)
cGATTATGAGCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAg < 1:4671205/76‑1 (MQ=255)
aTTATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAggg > 2:5767893/1‑75 (MQ=255)
ttATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAGCAGGTg > 2:105198/1‑76 (MQ=255)
ttATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTg < 2:346438/76‑1 (MQ=255)
ttATGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGAGAACAggag > 1:3698819/1‑74 (MQ=255)
aTGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGCACAGGTGCt > 2:1984995/1‑76 (MQ=255)
aTGATCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCt > 2:4910430/1‑76 (MQ=255)
aTCGCGATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGc < 1:1692874/76‑1 (MQ=255)
cgcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCt > 1:3358189/1‑76 (MQ=255)
cgcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCt < 1:3551182/76‑1 (MQ=255)
gcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTa < 1:1550481/76‑1 (MQ=255)
gcgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTa < 2:3986180/76‑1 (MQ=255)
cgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAt < 1:564067/76‑1 (MQ=255)
cgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTAt < 1:3953654/76‑1 (MQ=255)
cgATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAAAAGGTGCTGGCCTAt > 2:5871858/1‑76 (MQ=255)
gATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc > 1:995061/1‑76 (MQ=255)
gATGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATc < 1:1361838/76‑1 (MQ=255)
aTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATcc < 2:1158340/76‑1 (MQ=255)
aTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATcc < 2:1511597/76‑1 (MQ=255)
aTGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATcc > 2:1396868/1‑76 (MQ=255)
tGTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCg < 2:4687981/76‑1 (MQ=255)
gTCGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTTGCCTATcctg > 2:752381/1‑74 (MQ=255)
cGTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGc > 2:3310962/1‑76 (MQ=255)
gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGCACAGGTGCCGGCCTATCCGCGca > 1:2644572/1‑76 (MQ=255)
gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 2:4581647/1‑76 (MQ=255)
gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 1:5802098/1‑76 (MQ=255)
gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 1:2921071/1‑76 (MQ=255)
gTTATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGca > 2:112744/1‑76 (MQ=255)
tATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc > 1:5327827/1‑76 (MQ=255)
tATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc < 1:6190014/76‑1 (MQ=255)
tATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc > 1:1754612/1‑76 (MQ=255)
tATCATCAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacc > 1:5524427/1‑76 (MQ=255)
atcatcAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacca > 1:6096310/1‑76 (MQ=255)
atcatcAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacca < 2:4138849/76‑1 (MQ=255)
atcatcAGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGcacca < 1:1283514/76‑1 (MQ=255)
tcatcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGGGCTGGCCTATCCGGGcaccac > 1:187765/1‑76 (MQ=255)
catcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACg > 1:3814871/1‑76 (MQ=255)
atcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGc > 1:1155112/1‑76 (MQ=255)
tcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGct < 2:287352/76‑1 (MQ=255)
tcaGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGcc > 2:2539938/1‑75 (MQ=255)
aGCACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGctct < 1:4215022/76‑1 (MQ=255)
cACCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTAt < 2:1147829/76‑1 (MQ=255)
aCCCTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTAtt > 2:3785591/1‑76 (MQ=255)
ccTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCg < 1:3968196/76‑1 (MQ=255)
cTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGc < 2:1929746/76‑1 (MQ=255)
cTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGc > 1:6082428/1‑76 (MQ=255)
cTGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGc < 1:6050982/76‑1 (MQ=255)
tGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCt < 2:467679/76‑1 (MQ=255)
tGCATCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGCGCCGGCCTATCCGGGCACCACCCTCTATTCGCt > 1:5560117/1‑76 (MQ=255)
|
ACAGGCCTGACCGAAGGATCGACGCCAAACGGTAACGATTATGATCGCGATGTCGTTATCATCAGCACCCTGCGTCTCGACAAGCTGCACGTTCTTGGCAAGGGCGAACAGGTGCTGGCCTATCCGGGCACCACGCTCTATTCGCT > REL606/2173657‑2173802
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
