Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A108 F1 I1 R1
|
80 |
195.1 |
12639756 |
93.3% |
11792892 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,912,276 |
(T)8→7 |
intergenic (+56/‑84) |
ilvL → / → ilvG |
ilvG operon leader peptide/acetolactate synthase II, valine insensitive, large subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,912,269 | 0 | T | . | 94.0%
| 826.1
/ 43.8
| 185 | intergenic (+49/‑91) | ilvL/ilvG | ilvG operon leader peptide/acetolactate synthase II, valine insensitive, large subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/2); major base . (82/90); minor base G (8/3); total (90/95) |
| Fisher's exact test for biased strand distribution p-value = 1.29e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.01e-01 |
Only 100 of 216 total aligned reads displayed.
CTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATT > REL606/3912195‑3912341
|
cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtt < 2:580590/76‑1 (MQ=255)
cTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtt < 2:2552981/76‑1 (MQ=255)
gCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttt > 2:472094/1‑76 (MQ=255)
gCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttt < 2:4096987/76‑1 (MQ=255)
cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGttttt > 2:2030717/1‑76 (MQ=255)
cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGttttt < 2:2953766/76‑1 (MQ=255)
cACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGttttt > 2:4221348/1‑76 (MQ=255)
cTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGttttttt < 1:751239/76‑1 (MQ=255)
ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttg < 1:998613/76‑2 (MQ=255)
ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttg > 2:2717129/1‑75 (MQ=255)
ttGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGtttttttg < 2:4951114/76‑2 (MQ=255)
aCGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCt > 1:2458464/1‑76 (MQ=255)
cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCtt < 2:3188647/76‑1 (MQ=255)
cGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCtt < 2:1610143/76‑1 (MQ=255)
gAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTa < 2:4386792/76‑1 (MQ=255)
gAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTa < 2:2250677/76‑1 (MQ=255)
aGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTaa < 2:1049252/76‑1 (MQ=255)
ggAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTaaa < 1:5778633/76‑1 (MQ=255)
gAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTaaaa < 2:2135714/76‑1 (MQ=255)
aagggCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTaaaaa < 1:2360978/73‑1 (MQ=255)
aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTaaaaa > 1:212918/1‑76 (MQ=255)
aaaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTaaaaa < 2:447215/76‑1 (MQ=255)
aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAAc < 1:588428/76‑1 (MQ=255)
aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAAc < 1:4908491/76‑1 (MQ=255)
aaGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAAc > 2:638662/1‑76 (MQ=255)
aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACa < 1:2590122/76‑1 (MQ=255)
aGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACa < 1:1453829/76‑1 (MQ=255)
gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATa > 1:6081100/1‑76 (MQ=255)
gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATa < 1:1208290/76‑1 (MQ=255)
gCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATa > 1:2702816/1‑76 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATa < 1:4195348/76‑1 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATaa > 1:4764574/1‑76 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATaa < 1:1742522/76‑1 (MQ=255)
cTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATaa > 2:2324491/1‑76 (MQ=255)
ttAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAAc > 2:5163663/1‑76 (MQ=255)
tAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAAcc > 1:823650/1‑76 (MQ=255)
agagATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCg > 2:5639839/1‑76 (MQ=255)
agaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCgag > 1:1403513/1‑76 (MQ=255)
gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCgagg < 1:3468730/76‑1 (MQ=255)
gaTCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCgagg < 2:2891797/76‑1 (MQ=255)
cAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCgag < 2:4786292/72‑1 (MQ=255)
cAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCgag > 1:4786292/1‑72 (MQ=255)
aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCa > 2:1882469/1‑76 (MQ=255)
aaGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCa > 2:1348894/1‑76 (MQ=255)
aGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAg > 2:5329558/1‑76 (MQ=255)
gCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGa < 1:5374660/76‑1 (MQ=255)
cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa < 2:3769035/76‑1 (MQ=255)
cTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACa > 2:6071266/1‑76 (MQ=255)
ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa > 1:4532718/1‑76 (MQ=255)
ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa < 1:3155995/76‑1 (MQ=255)
ttAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACaa > 2:6292804/1‑76 (MQ=255)
tAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAAt < 2:5379749/76‑1 (MQ=255)
aaCGAACTAAGACCCCCGCACCGAAGGGTCCGGGGG‑TTTTTTTGACCTTAAAACCATAACCGAGGAGCAGACAATg < 2:5694316/76‑1 (MQ=255)
cGAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGaa < 1:2049881/76‑1 (MQ=255)
gAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt > 1:5327678/1‑76 (MQ=255)
gAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt < 1:2305799/76‑1 (MQ=255)
gAACTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAAt > 1:1524974/1‑76 (MQ=255)
aaCTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATa > 1:3009133/1‑76 (MQ=255)
aCTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa < 1:3955156/76‑1 (MQ=255)
aCTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa > 1:74393/1‑76 (MQ=255)
aCTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa < 1:2820904/76‑1 (MQ=255)
aCTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATaa < 2:3870394/76‑1 (MQ=255)
cTAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAAc > 2:1255271/1‑76 (MQ=255)
tAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa > 2:2078511/1‑76 (MQ=255)
tAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa < 1:2882310/76‑1 (MQ=255)
tAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa > 1:2998722/1‑76 (MQ=255)
tAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa < 2:1960220/76‑1 (MQ=255)
tAAGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACa < 2:1284716/76‑1 (MQ=255)
tAAGACCCCAGCACAGAAAGGGCAGGTGG‑TTTTTTTGACCTGAAAAACATAGCCGTGGAGCAGACAATGAATAACa < 2:5831952/76‑1 (MQ=255)
aaGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAg < 2:2501903/76‑1 (MQ=255)
aGACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGc > 2:4512604/1‑76 (MQ=255)
gACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca < 2:2447744/76‑1 (MQ=255)
gACCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGca > 1:5238334/1‑76 (MQ=255)
aCCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcac > 1:106726/1‑76 (MQ=255)
aCCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcac > 2:756254/1‑76 (MQ=255)
aCCCCCGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGcac > 2:3361896/1‑76 (MQ=255)
ccccGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaa > 1:4290419/1‑76 (MQ=255)
ccccGCACCGAAAGGTCC‑GGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaa < 1:5077322/76‑1 (MQ=255)
cccGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaa < 2:4901679/76‑1 (MQ=255)
ccGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaaa < 1:1255734/76‑1 (MQ=255)
ccGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACaaaa < 1:3610584/76‑1 (MQ=255)
cGCACCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAAt < 2:2175722/76‑1 (MQ=255)
gCACAGAAAGGTCC‑GCGCTTTATTTTGACCTTACGATCATATCAGAGGAGCAGACAATGAATAACATCACAAAAtt < 2:328198/76‑1 (MQ=255)
aCCGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCt > 2:5603727/1‑76 (MQ=255)
ccGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg > 2:3980490/1‑76 (MQ=255)
ccGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTg > 1:5288170/1‑76 (MQ=255)
cGAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGt > 1:4348990/1‑76 (MQ=255)
gAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt > 1:3177294/1‑76 (MQ=255)
gAAAGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGtt < 2:3601063/76‑1 (MQ=255)
aaGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtc > 2:1371748/1‑76 (MQ=255)
aGGTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtct > 2:5543029/1‑76 (MQ=255)
ggTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTtctc < 2:4047900/76‑1 (MQ=255)
gTCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCa > 1:5359196/1‑76 (MQ=255)
tCCGGGGG‑TTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCaa > 2:5435147/1‑76 (MQ=255)
ccggggGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg > 2:5471056/3‑76 (MQ=255)
ccggggGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg > 2:3103383/3‑76 (MQ=255)
ccggggGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAg < 1:5641632/74‑1 (MQ=255)
ggggGTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAt > 2:279036/1‑76 (MQ=255)
ggggTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt < 1:3032849/76‑1 (MQ=255)
ggggTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGAtt < 2:2384624/76‑1 (MQ=255)
|
CTGCACTTGGACGAGGAAAGGCTTAGAGATCAAGCCTTAACGAACTAAGACCCCCGCACCGAAAGGTCCGGGGGTTTTTTTTGACCTTAAAAACATAACCGAGGAGCAGACAATGAATAACAGCACAAAATTCTGTTTCTCAAGATT > REL606/3912195‑3912341
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
