Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A108 F1 I1 R1
|
80 |
195.1 |
12639756 |
93.3% |
11792892 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,302,867 |
A→T |
D100E (GAT→GAA) |
phnD ← |
phosphonate/organophosphate ester transporter subunit |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,302,867 | 0 | A | T | 99.2%
| 487.3
/ NA
| 134 | D100E (GAT→GAA) | phnD | phosphonate/organophosphate ester transporter subunit |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (90/43); minor base G (1/0); total (91/43) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 152 total aligned reads displayed.
CGATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGATCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATA > REL606/4302794‑4302939
|
cGATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTc > 1:1867631/1‑76 (MQ=255)
gATCACGACGCCCCAGTAACCTGGCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTcc < 1:2545545/76‑1 (MQ=255)
gATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTcc > 2:1605755/1‑76 (MQ=255)
aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCa > 2:4543644/1‑76 (MQ=255)
aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCa > 1:3899628/1‑76 (MQ=255)
aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCa > 2:2407588/1‑76 (MQ=255)
aTCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGACGTTGGAGCGTTCCa > 2:5556791/1‑76 (MQ=255)
tCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCAc > 2:2415240/1‑76 (MQ=255)
aaCACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGGTCCACCg > 2:1440113/1‑76 (MQ=255)
gcGCTCCAGTAACCGGCCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCtt < 2:2624865/75‑1 (MQ=255)
ccGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCtt < 1:4708853/75‑1 (MQ=255)
acGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCtt > 2:775797/1‑76 (MQ=255)
cGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTc > 1:4420845/1‑76 (MQ=255)
cGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTc > 1:6010368/1‑76 (MQ=255)
gCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTcc > 1:4956027/1‑76 (MQ=255)
tCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAt > 1:3005802/1‑76 (MQ=255)
cccAGTACCCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAt < 2:605039/75‑1 (MQ=255)
ccAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAtt > 1:3481665/1‑76 (MQ=255)
ccAGTAACCGGGCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCAtt < 1:5703253/76‑1 (MQ=255)
cAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTg > 2:3491058/1‑76 (MQ=255)
aGTAGCCGGGCGATCCACCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGc < 1:4191235/76‑1 (MQ=255)
aGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTACACCGCTTCCCTTGc > 1:2694035/1‑76 (MQ=255)
gTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGcc > 2:4230963/1‑76 (MQ=255)
gTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGcc > 1:4298945/1‑76 (MQ=255)
tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCg > 1:4965384/1‑76 (MQ=255)
tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCg > 2:274956/1‑76 (MQ=255)
tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCg > 1:1078327/1‑76 (MQ=255)
tAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCAACGCTTCCATTGCCg > 1:4055840/1‑76 (MQ=255)
aaCCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGa > 2:2749016/1‑76 (MQ=255)
aaCCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGa > 2:3549315/1‑76 (MQ=255)
ccgggCGATCCATCCGCCGCGACCGTCTGGGCGAAGGCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACa < 1:2688872/73‑1 (MQ=255)
ccTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGGCa > 2:3363938/1‑76 (MQ=255)
ccTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACa > 1:5593142/1‑76 (MQ=255)
ccTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGACGACa > 1:3794449/1‑76 (MQ=255)
cTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAg > 1:4344784/1‑76 (MQ=255)
cTGGCGACCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAg < 2:2659754/76‑1 (MQ=255)
ggCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGt > 2:235390/1‑76 (MQ=255)
gATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGc > 2:1716845/1‑76 (MQ=255)
tgccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGcc < 1:4903369/74‑1 (MQ=255)
cccacccGCGGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCg < 2:1078327/75‑1 (MQ=255)
ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCgg > 1:2147186/1‑75 (MQ=255)
ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 1:3009575/1‑76 (MQ=255)
ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 2:3410820/1‑76 (MQ=255)
ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 1:3702046/1‑76 (MQ=255)
ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGt > 2:3841738/1‑76 (MQ=255)
ccatccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGGTGCCGt > 1:466497/1‑76 (MQ=255)
atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:2074634/1‑76 (MQ=255)
atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:6181431/1‑76 (MQ=255)
atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:5635243/1‑76 (MQ=255)
atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 1:715585/1‑76 (MQ=255)
atccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAc > 2:112766/1‑76 (MQ=255)
tccGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTAcc < 1:5466463/76‑1 (MQ=255)
ccgccgCGCCCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 1:5905585/76‑1 (MQ=255)
ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa > 1:1454500/1‑76 (MQ=255)
ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 2:4764947/76‑1 (MQ=255)
ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 1:4923473/76‑1 (MQ=255)
ccgccgCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCa < 1:6246374/76‑1 (MQ=255)
cgccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAg > 1:3300960/1‑76 (MQ=255)
gccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAgg < 2:656879/76‑1 (MQ=255)
ccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCCGGc > 2:1475442/1‑76 (MQ=255)
ccgcGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGc > 1:5565847/1‑76 (MQ=255)
cgcgCCCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCg < 2:2313042/76‑1 (MQ=255)
gcccGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGata < 2:6267463/74‑1 (MQ=255)
gACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGTGatt > 1:4094301/1‑75 (MQ=255)
gACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGata > 1:2355561/1‑76 (MQ=255)
cGTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATcc > 1:5667388/1‑76 (MQ=255)
gTCTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCa > 1:2877261/1‑76 (MQ=255)
gTCGGGGCGAAGCCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCa < 1:775797/76‑1 (MQ=255)
tcggggCGAAGCCCTGGCCGTTGGCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCAc < 2:5593142/73‑1 (MQ=255)
cTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACt > 2:2105489/1‑76 (MQ=255)
cTGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACt > 2:6278353/1‑76 (MQ=255)
tGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACtt > 2:4798978/1‑76 (MQ=255)
tGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACtt < 2:2196153/76‑1 (MQ=255)
tGGGCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACtt > 1:2637697/1‑76 (MQ=255)
gggCGAAGCCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACttt < 1:5405311/76‑1 (MQ=255)
ggCAAAGACCTGGCGGTTGGCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTa < 1:955150/76‑1 (MQ=255)
gCGAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTAt > 1:2931772/1‑76 (MQ=255)
gAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTg > 2:3813066/1‑76 (MQ=255)
gAAGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTg > 1:3398029/1‑76 (MQ=255)
aaGACCTGGCCGTTGGCGCGTTCCGCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:3353103/1‑76 (MQ=255)
aaGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:1159734/1‑76 (MQ=255)
aaGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:5232621/1‑76 (MQ=255)
aaGACCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGa > 2:135073/1‑76 (MQ=255)
aggccTGGCCGTTGGCGCGTTCCACCGCTCCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGaa < 2:2944234/73‑1 (MQ=255)
gcccTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAg < 2:1852943/74‑1 (MQ=255)
aCCTGGTCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAgc > 1:3479382/1‑76 (MQ=255)
aCCTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAgc < 1:2304473/76‑1 (MQ=255)
cTGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAgcgc > 1:1678477/1‑76 (MQ=255)
tGGCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCa > 2:1664180/1‑76 (MQ=255)
ggCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCAt > 1:848797/1‑76 (MQ=255)
gCCGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCAtt < 2:658318/76‑1 (MQ=255)
ccGTTGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTc < 2:3556637/76‑1 (MQ=255)
cGTTGGCGCTTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTcc < 1:5399280/76‑1 (MQ=255)
gtgggCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTccc < 2:3719610/73‑1 (MQ=255)
ttGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTCCCGGGCGATATCCACTTTATTGAAGCGCATTCCCt < 2:5461155/76‑1 (MQ=255)
tgggCGCGTTCCCCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCt < 2:1885839/74‑1 (MQ=255)
tGGCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTg > 1:1514467/1‑76 (MQ=255)
gggCGCGTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTg < 2:3389433/75‑1 (MQ=255)
gcgTTCCGCCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATa < 1:1099261/76‑1 (MQ=255)
gcgTTCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATa < 2:5974834/76‑1 (MQ=255)
|
CGATCAACACGCTCCAGTAACCTGGCGATCCATCCGCCGCGACCGTCTGGGCGAAGACCTGGCCGTTGGCGCGATCCACCGCTTCCATTGCCGACAGGTTGCCGTACCAGGCGATATCCACTTTATTGAAGCGCATTCCCTGGATA > REL606/4302794‑4302939
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
