Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
19 |
34.1 |
2156969 |
96.4% |
2079318 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,249,887 |
C→T |
M454I (ATG→ATA) |
infB ← |
translation initiation factor IF‑2 |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,249,887 | 0 | C | T | 100.0%
| 100.7
/ NA
| 29 | M454I (ATG→ATA) | infB | translation initiation factor IF‑2 |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (11/18); total (11/18) |
TGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGT > REL606/3249816‑3249954
|
tGCGGCATCACACCGTCGTCGGCAGCAGCAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTg < 1:628254/76‑1 (MQ=255)
cATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTa < 2:639814/76‑1 (MQ=255)
cATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTa < 1:596409/76‑1 (MQ=255)
aTCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTaa < 2:514707/76‑1 (MQ=255)
aTCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTaa < 1:720560/76‑1 (MQ=255)
ccacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcg < 1:176341/75‑1 (MQ=255)
acacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcg < 2:1028449/76‑1 (MQ=255)
acacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcg > 1:886255/1‑76 (MQ=255)
cacCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAAcgc < 1:127728/76‑1 (MQ=255)
ccGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAGGTAAAggcgc > 2:444534/1‑75 (MQ=255)
cagtcgtcGGCAGCAACAACCAGGACTACGATGTCCGTAGCCTGCGCACCACGCGCACGTATTGAAGTAAACgcgg > 1:795850/3‑76 (MQ=255)
cgtcgGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTg < 2:377773/76‑1 (MQ=255)
agcaACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCggg < 2:74112/76‑1 (MQ=255)
gcaACAACCACGACGCTGTTGTCAGTTGCCTGAGCACCACAAGCATGTATTGAAGTAAACGCGGCGTGCCCCgggg < 2:446123/76‑1 (MQ=255)
aacaacCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGgtg < 1:465242/76‑1 (MQ=255)
aacCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTcc > 1:266253/1‑76 (MQ=255)
gACTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTGCACGCGGCGTGCCCCGGGGTGTCCAGGAAg < 1:1064220/76‑1 (MQ=255)
aCTACGATGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAgg > 1:68193/1‑76 (MQ=255)
tGTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCAt < 2:44620/76‑1 (MQ=255)
gTCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATg > 1:821666/1‑76 (MQ=255)
tCCGTTGCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGc < 1:439982/76‑1 (MQ=255)
gCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGtttt < 1:1016798/76‑1 (MQ=255)
gCCTGCGCACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGtttt > 1:463270/1‑76 (MQ=255)
ccTGCGCNCCACGAGCACGTACTGNAGTAAACGCGGCGTGCNCCGGGGTGATCAGGAAGGTGATCATGCCGTTTTc < 2:962265/76‑1 (MQ=255)
tGCACACCACGAGCACGTATTGAAGTAAACGCGGCGTGCCCCAGGGTGTCCAGGAAGNTGATCATGCCGTTTTCAg > 1:844098/1‑76 (MQ=255)
cgcACCACGAGCANGTATTGNAGTAATCGCGGCGTGNCCCGGGGTGTCCAGNGAGGTGATCANGCCGTTTTCAGtt < 2:928508/76‑1 (MQ=255)
caccacGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTc > 2:308280/1‑76 (MQ=255)
accacGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATAATGCCGTTTTCAGTTTCa > 1:820812/1‑76 (MQ=255)
acaacGAGCACGTATTGAAGTAAACGCGCCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCa > 2:11002/4‑76 (MQ=255)
acGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACg > 2:1068408/1‑76 (MQ=255)
cGAGCACGTATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGt < 1:533731/76‑1 (MQ=255)
|
TGCGGCATCACACCGTCGTCGGCAGCAACAACCAGGACTACGATGTCCGTTGCCTGCGCACCACGAGCACGCATTGAAGTAAACGCGGCGTGCCCCGGGGTGTCCAGGAAGGTGATCATGCCGTTTTCAGTTTCAACGT > REL606/3249816‑3249954
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
