Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A5 F1 I1 R1
|
19 |
34.1 |
2156969 |
96.4% |
2079318 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,326,561 |
A→T |
I69N (ATC→AAC) |
mreD ← |
cell wall structural complex MreBCD transmembrane component MreD |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,326,561 | 0 | A | T | 100.0%
| 116.8
/ NA
| 33 | I69N (ATC→AAC) | mreD | cell wall structural complex MreBCD transmembrane component MreD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (17/16); total (17/16) |
CTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCA > REL606/3326488‑3326631
|
cTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTc > 1:181123/1‑76 (MQ=255)
gTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGa < 1:1028623/76‑1 (MQ=255)
gTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGa < 1:374526/76‑1 (MQ=255)
tttCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATcc < 2:102724/76‑1 (MQ=255)
ttCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCa > 2:385765/1‑76 (MQ=255)
ttCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGAACGCCAAGCGTCGAGCCGCTGTTCAGATCCa > 2:996769/1‑76 (MQ=255)
tCAGCGCCACCAGGTAANCAATGATGCTCATCGCCAATACGCGTACGCCAANCGTCGACCCGCCGTTCAGATCCAg > 2:898448/1‑76 (MQ=255)
acgcCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTNAGATCCTGTAt < 1:153686/75‑1 (MQ=255)
cgcCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATg > 2:1527/1‑76 (MQ=255)
cgcCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATg < 1:98022/76‑1 (MQ=255)
gcCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATgg < 1:463362/76‑1 (MQ=255)
aGGTAAGCAATGACGCCCACCGCCAAAACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCa < 1:260553/76‑1 (MQ=255)
tAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATca > 1:887478/1‑76 (MQ=255)
cAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACaaa < 1:545937/76‑1 (MQ=255)
cAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACaaa > 2:388468/1‑76 (MQ=255)
aaTGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACaaaa > 2:29604/1‑76 (MQ=255)
tgatgCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACNAAAcc > 2:876102/1‑76 (MQ=255)
tgatgCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAAcc > 2:612117/1‑76 (MQ=255)
cATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGccc > 2:389313/1‑76 (MQ=255)
cGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCcaca < 1:898652/76‑1 (MQ=255)
cGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCcaca > 2:1071770/1‑76 (MQ=255)
cGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCcaca > 2:603885/1‑76 (MQ=255)
aaTACGCGTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTa < 1:333232/76‑1 (MQ=255)
cgTGCGCCCACCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGAt < 2:12069/76‑1 (MQ=255)
cgTACGCCAAGCGTCGAGCCGCTNTTCAGATNCNGTATGGAACCCCTCACAAAACCTGAGCCCAGATTTATGCGAt > 1:1004489/1‑76 (MQ=255)
gTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGTCCACATTTACGCGATg > 2:443872/1‑76 (MQ=255)
gTACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATg > 1:740953/1‑76 (MQ=255)
tACGCCAAGCGTCGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGa < 2:509252/76‑1 (MQ=255)
aaGCGGCGAGCCGCTGTTCAGATCCAGTATGGCTCCCATCACAAAACCTGTGCTCTCATTTACGCGCTGAGGCAAg < 1:107019/76‑1 (MQ=255)
gtggAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCa < 2:1029804/73‑1 (MQ=255)
cGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAgg < 2:311768/76‑1 (MQ=255)
cGAGCCGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAgg > 1:594006/1‑76 (MQ=255)
cGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa < 2:226320/76‑1 (MQ=255)
cGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa > 2:206051/1‑76 (MQ=255)
cGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa > 2:642455/1‑76 (MQ=255)
cGCTGTTCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCa < 1:799157/76‑1 (MQ=255)
|
CTGGTATTTCAGCGCCACCAGGTAAGCAATGATGCTCATCGCCAATACGCGTACGCCAAGCGTCGAGCCGCTGATCAGATCCAGTATGGCACCCATCACAAAACCTGTGCCCACATTTACGCGATGAGGCAAGGCCAGGATCCA > REL606/3326488‑3326631
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
