Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A136 F1 I1 R1 21 250.8 15742942 95.7% 15065995 76.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA REL606 3,923,363 G→A P295P (CCG→CCA)  rep → DNA helicase and single‑stranded DNA‑dependent ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6063,923,3630GA100.0% 817.4 / NA 196P295P (CCG→CCA) repDNA helicase and single‑stranded DNA‑dependent ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (90/106);  total (90/106)

Only 100 of 211 total aligned reads displayed.

TTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATAAC  >  REL606/3923290‑3923435
                                                                         |                                                                        
ttAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCcaca                                                                        <  1:7069135/76‑1 (MQ=255)
ttAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCcaca                                                                        <  1:7234893/76‑1 (MQ=255)
ttAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCcaca                                                                        >  2:2831560/1‑76 (MQ=255)
 tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCacac                                                                       >  2:3418245/1‑76 (MQ=255)
 tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCacac                                                                       >  1:286588/1‑76 (MQ=255)
 tAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCacac                                                                       >  2:1361997/1‑76 (MQ=255)
  aaGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACg                                                                      >  1:1720401/1‑76 (MQ=255)
    gCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTc                                                                    <  1:7777529/76‑1 (MQ=255)
     cTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCt                                                                   <  2:7172696/76‑1 (MQ=255)
      ttGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCtt                                                                  >  1:1362208/1‑76 (MQ=255)
       tGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCttt                                                                 >  1:1342865/1‑76 (MQ=255)
       tGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCttt                                                                 >  2:16978/1‑76 (MQ=255)
       tGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCttt                                                                 >  2:1028611/1‑76 (MQ=255)
        gAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTg                                                                <  1:474400/76‑1 (MQ=255)
        gAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTg                                                                >  2:3221634/1‑76 (MQ=255)
         aGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGa                                                               <  2:476328/76‑1 (MQ=255)
          gCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGaa                                                              <  1:7443684/76‑1 (MQ=255)
           cAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGaaa                                                             <  1:1466180/76‑1 (MQ=255)
            aGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGaaaa                                                            <  1:814003/76‑1 (MQ=255)
            aGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGaaaa                                                            <  2:7542098/76‑1 (MQ=255)
            aGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGaaaa                                                            >  2:3443555/1‑76 (MQ=255)
             gAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAg                                                           <  2:2656707/76‑1 (MQ=255)
              aaCTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGc                                                          >  1:2293759/1‑76 (MQ=255)
              aaCTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGc                                                          >  1:5951947/1‑76 (MQ=255)
                cTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGt                                                        >  1:3560494/1‑76 (MQ=255)
                 tACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTc                                                       >  1:2638699/1‑76 (MQ=255)
                 tACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTc                                                       >  1:5272465/1‑76 (MQ=255)
                  aCCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCt                                                      <  1:1206504/76‑1 (MQ=255)
                     gCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGtt                                                   <  2:6954959/76‑1 (MQ=255)
                      ctctTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTtc                                                  >  2:2162659/1‑76 (MQ=255)
                       tctTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTtct                                                 <  2:6335999/76‑1 (MQ=255)
                        ctTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTtctc                                                <  2:5493055/76‑1 (MQ=255)
                         ttCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTcc                                               >  2:3591004/1‑76 (MQ=255)
                         tgccGGACGTATTTGGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTcc                                               <  1:6848102/74‑1 (MQ=255)
                          tCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCg                                              >  1:1166551/1‑76 (MQ=255)
                          tCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCg                                              <  2:512588/76‑1 (MQ=255)
                           ccGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGa                                             >  2:2929870/1‑76 (MQ=255)
                           ccGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGa                                             >  2:4321194/1‑76 (MQ=255)
                            cGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGaa                                            <  2:2486842/76‑1 (MQ=255)
                              gACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACt                                          >  1:6158886/1‑76 (MQ=255)
                              gACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACt                                          <  2:7387117/76‑1 (MQ=255)
                              gACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACt                                          >  1:3242433/1‑76 (MQ=255)
                               aCGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTg                                         >  2:1617558/1‑76 (MQ=255)
                               aCGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTg                                         >  1:2773127/1‑76 (MQ=255)
                               aCGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTg                                         <  2:2011176/76‑1 (MQ=255)
                                cGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTgg                                        <  1:4853096/76‑1 (MQ=255)
                                  tATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGt                                      <  1:5083327/76‑1 (MQ=255)
                                  tATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGt                                      >  2:764032/1‑76 (MQ=255)
                                   aTTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGtt                                     >  1:1962616/1‑76 (MQ=255)
                                   aTTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGtt                                     <  1:2753452/76‑1 (MQ=255)
                                     tttGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTAc                                   <  1:3412918/76‑1 (MQ=255)
                                     tttGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTAc                                   <  2:426589/76‑1 (MQ=255)
                                      ttGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACg                                  <  1:1566153/76‑1 (MQ=255)
                                      ttGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACg                                  >  1:6561054/1‑76 (MQ=255)
                                       tGAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACgg                                 >  1:560987/1‑76 (MQ=255)
                                        gAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGc                                <  1:2596399/76‑1 (MQ=255)
                                        gAAAGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGc                                >  2:4970096/1‑76 (MQ=255)
                                         aaaGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca                               >  2:2582133/1‑76 (MQ=255)
                                         aaaGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca                               >  2:7135488/1‑76 (MQ=255)
                                         aaaGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca                               >  2:5785376/1‑76 (MQ=255)
                                         aaaGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGca                               >  2:5918440/1‑76 (MQ=255)
                                          aaGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcac                              <  1:4027259/76‑1 (MQ=255)
                                          aaGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcac                              <  1:628639/76‑1 (MQ=255)
                                           aGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcaca                             <  2:1804504/76‑1 (MQ=255)
                                           aGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcaca                             <  1:4461210/76‑1 (MQ=255)
                                           aGCGGCGAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGcaca                             <  1:3709896/76‑1 (MQ=255)
                                            gcggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACag                            <  1:6687413/76‑1 (MQ=255)
                                            gcggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACag                            <  2:600187/76‑1 (MQ=255)
                                            gcggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACag                            <  1:2356421/76‑1 (MQ=255)
                                             cggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACaga                           <  2:6472789/76‑1 (MQ=255)
                                             cggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACaga                           <  2:6644354/76‑1 (MQ=255)
                                             cggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACaga                           <  1:3952189/76‑1 (MQ=255)
                                              ggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACagag                          <  2:3258584/76‑1 (MQ=255)
                                              ggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACagag                          <  2:3672524/76‑1 (MQ=255)
                                              ggcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACagag                          <  2:6809278/76‑1 (MQ=255)
                                               gcgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGc                         <  2:6116501/76‑1 (MQ=255)
                                                cgAACATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCt                        >  2:2096740/1‑76 (MQ=255)
                                                  aaCATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaa                      >  2:1178167/1‑76 (MQ=255)
                                                  aaCATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaa                      >  2:3629820/1‑76 (MQ=255)
                                                  aaCATCCTGAGCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaa                      <  2:4068565/76‑1 (MQ=255)
                                                   aCATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaaa                     <  1:6062586/76‑1 (MQ=255)
                                                    cATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaaaa                    <  2:816052/76‑1 (MQ=255)
                                                    cATCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTaaaa                    <  1:3817083/76‑1 (MQ=255)
                                                     aTCCTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAg                   >  2:3843488/1‑76 (MQ=255)
                                                       ccTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGtt                 >  1:3310357/1‑76 (MQ=255)
                                                        cTGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGttt                >  1:6609161/1‑76 (MQ=255)
                                                         tGATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGtttt               >  2:5221037/1‑76 (MQ=255)
                                                          gATCGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTa              <  1:3029039/76‑1 (MQ=255)
                                                             cGCCAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAgc           >  1:1408103/1‑76 (MQ=255)
                                                                cAATAATCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGcgcg        <  2:7454249/76‑1 (MQ=255)
                                                                 aataatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGa       >  2:7797660/1‑76 (MQ=255)
                                                                 aataatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGa       <  1:2054800/76‑1 (MQ=255)
                                                                   taatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAAt     >  2:929957/1‑76 (MQ=255)
                                                                   taatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAAt     <  1:755976/76‑1 (MQ=255)
                                                                   taatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAAt     <  1:7771919/76‑1 (MQ=255)
                                                                    aatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATa    <  1:6642619/76‑1 (MQ=255)
                                                                    aatCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATa    <  2:2917178/76‑1 (MQ=255)
                                                                     atCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATaa   <  2:4498010/76‑1 (MQ=255)
                                                                     atCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATaa   <  2:3824811/76‑1 (MQ=255)
                                                                      tCCACACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATAAc  <  1:2401616/76‑1 (MQ=255)
                                                                         |                                                                        
TTAAGCTTGAGCAGAACTACCGCTCTTCCGGACGTATTTTGAAAGCGGCGAACATCCTGATCGCCAATAATCCGCACGTCTTTGAAAAGCGTCTGTTCTCCGAACTGGGTTACGGCACAGAGCTAAAAGTTTTAAGCGCGAATAAC  >  REL606/3923290‑3923435

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A