Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A45 F1 I1 R1
|
14 |
53.1 |
3307936 |
97.7% |
3231853 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,927,357 |
(T)8→9 |
coding (74/1266 nt) |
rhlB ← |
ATP‑dependent RNA helicase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,927,349 | 1 | . | T | 100.0%
| 161.8
/ NA
| 40 | G28R (GGG→AGG) | rhlB | ATP‑dependent RNA helicase |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (25/15); total (25/15) |
CTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATG > REL606/3927275‑3927418
|
cTGCCCGGCTACGTCACGCCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑t < 2:367421/76‑1 (MQ=255)
cTGCCCGGCTACGTCACGCCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑t < 2:590541/76‑1 (MQ=255)
cccGGCTCCGTCACGCCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑tttt < 2:925008/76‑1 (MQ=255)
ccGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttt < 1:644947/76‑1 (MQ=255)
ccGCCTCCGGCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttt < 2:113115/76‑1 (MQ=255)
cgacgccacgccCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑ttttttttt < 2:1338687/74‑2 (MQ=255)
tACGCCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑tttttttttc < 1:319292/76‑3 (MQ=255)
tCACGGCCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAgg < 1:1327849/76‑1 (MQ=255)
tCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAgg > 2:1243834/1‑76 (MQ=255)
tCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAgg > 2:807435/1‑76 (MQ=255)
aCGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGc > 1:881651/1‑76 (MQ=255)
aCGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGc > 1:1144320/1‑76 (MQ=255)
gACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCTTTTTTTTTTCAAGGGCt > 2:622403/1‑76 (MQ=255)
cccccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCtt < 1:1163201/74‑1 (MQ=255)
ccccccAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCt < 2:24617/76‑1 (MQ=255)
ccgccgGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTa < 1:331810/76‑1 (MQ=255)
ccGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTa < 1:1013413/76‑1 (MQ=255)
gCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACa > 2:367116/1‑76 (MQ=255)
ccAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACaa < 1:160393/76‑1 (MQ=255)
gCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCt > 2:204897/1‑76 (MQ=255)
cGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATg > 2:1365978/1‑76 (MQ=255)
cGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATg > 1:1479596/1‑76 (MQ=255)
ggAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGc > 2:762026/1‑76 (MQ=255)
ggAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGc > 1:113675/1‑76 (MQ=255)
aGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg < 2:1160242/76‑1 (MQ=255)
aGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg > 2:1051661/1‑76 (MQ=255)
aGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAgg < 1:641408/76‑1 (MQ=255)
gCCAGTGCCTGGATGGGGCTACAGTTAAGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCg < 2:1417613/76‑1 (MQ=255)
cAGTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGaa < 1:602631/76‑1 (MQ=255)
gTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGt > 1:1272719/1‑76 (MQ=255)
gTGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGt < 2:377316/76‑1 (MQ=255)
tGCCTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTc < 1:858212/76‑1 (MQ=255)
ccTGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCgg < 1:1019179/76‑1 (MQ=255)
tGAATGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGaa > 2:336174/1‑76 (MQ=255)
aaTGGGCGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACATCCTTCGGATGCAGGGCGAAGTCGGaaaa < 2:109623/76‑1 (MQ=255)
cGTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctg > 2:452090/1‑76 (MQ=255)
gTACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgt > 1:1067054/1‑76 (MQ=255)
tACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgtt > 2:498052/1‑76 (MQ=255)
tACAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgtt < 1:1311823/76‑1 (MQ=255)
cAGTTATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACttctgttct > 1:1572690/1‑76 (MQ=255)
ttATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTctgttctgtt > 1:141650/1‑76 (MQ=255)
ttATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTctgttctgtt > 2:1010792/1‑76 (MQ=255)
tATGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTa < 1:30117/76‑1 (MQ=255)
tGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaaa > 2:1323337/1‑76 (MQ=255)
tGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaaa > 1:122744/1‑76 (MQ=255)
tGAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTaaa > 2:1255645/1‑76 (MQ=255)
gAAACCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAt > 2:605176/1‑76 (MQ=255)
aaaCCCT‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAAtg > 1:1131818/1‑76 (MQ=255)
|
CTGCCCGGCTACGTCACGACCCGCCAGCGTCAGCGGAAGGGCCAGTGCCTGAATGGGCGTACAGTTATGAAACCC‑‑TTTTTTTTCAAGGGCTTCTACAACCTTCGGATGCAGGGCGAAGTCGGAAAACTTCTGTTCTGTTAAATG > REL606/3927275‑3927418
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
