Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A14 F1 I1 R1
|
17 |
21.6 |
1356596 |
98.4% |
1334890 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,204 |
A→G |
intergenic (+72/‑13) |
rhoL → / → rho |
rho operon leader peptide/transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,204 | 0 | A | G | 100.0%
| 90.4
/ NA
| 23 | intergenic (+72/‑13) | rhoL/rho | rho operon leader peptide/transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (12/11); total (12/11) |
GACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAA > REL606/3928131‑3928274
|
gACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGAc > 1:671326/1‑76 (MQ=255)
tAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTcaca > 2:582368/1‑76 (MQ=255)
aaaCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCacac > 2:339046/1‑76 (MQ=255)
aaCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCAcacc > 2:503703/1‑76 (MQ=255)
aCAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCAcacca < 1:194614/76‑1 (MQ=255)
cAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCAcaccac > 2:493777/1‑76 (MQ=255)
gCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTAt < 1:229557/76‑1 (MQ=255)
gCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTAt < 2:441608/76‑1 (MQ=255)
aTGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGa < 1:454949/76‑1 (MQ=255)
gatgatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATc < 1:40132/76‑1 (MQ=255)
gatCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTa < 2:126567/76‑1 (MQ=255)
cTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGa > 2:540427/1‑76 (MQ=255)
cATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTa > 2:410478/1‑76 (MQ=255)
cacaACATTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACg > 1:550749/1‑76 (MQ=255)
aTTAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGtt > 2:520293/1‑76 (MQ=255)
ttAAGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGttt > 1:201789/1‑76 (MQ=255)
aGTTCGAGATTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTg < 2:363105/76‑1 (MQ=255)
gagaTTTACCCCAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTg > 2:641015/1‑76 (MQ=255)
ccAAGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCg < 2:51549/76‑1 (MQ=255)
aGTTTAAGGACTCACACCACTATGAATCTTATCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCg < 1:557842/76‑1 (MQ=255)
aGTTTAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCg < 1:352932/76‑1 (MQ=255)
tttAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaa < 1:133464/76‑1 (MQ=255)
ttAAGGACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGaaa > 1:102750/1‑76 (MQ=255)
|
GACCGTAAACAGGCATGGATGATCCTGCCATACCATTCACAACATTAAGTTCGAGATTTACCCCAAGTTTAAGAACTCACACCACTATGAATCTTACCGAATTAAAGAATACGCCGGTTTCTGAGCTGATCACTCTCGGCGAAA > REL606/3928131‑3928274
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
