Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A119 F1 I1 R1
|
20 |
247.5 |
15984500 |
92.7% |
14817631 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,697,996 |
T→G |
D8A (GAT→GCT) |
slyA ← |
transcriptional regulator SlyA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,697,996 | 0 | T | G | 98.4%
| 1064.2
/ 3.1
| 247 | D8A (GAT→GCT) | slyA | transcriptional regulator SlyA |
| Reads supporting (aligned to +/- strand): ref base T (3/1); new base G (100/143); total (103/144) |
| Fisher's exact test for biased strand distribution p-value = 3.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01 |
Only 100 of 255 total aligned reads displayed.
TGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAT > REL606/1697921‑1698068
|
gggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATc < 1:1136937/76‑1 (MQ=255)
ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa < 2:7914698/76‑1 (MQ=255)
ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa > 2:7425903/1‑76 (MQ=255)
ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa < 1:5864271/76‑1 (MQ=255)
ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa < 1:5650450/76‑1 (MQ=255)
ggTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCa > 1:2156185/1‑76 (MQ=255)
gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg > 1:1380506/1‑76 (MQ=255)
gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg > 2:4168097/1‑76 (MQ=255)
gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg < 1:4099659/76‑1 (MQ=255)
gTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAg > 2:3310003/1‑76 (MQ=255)
tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGa < 1:7845179/76‑1 (MQ=255)
tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGa > 2:6947820/1‑76 (MQ=255)
tttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGa < 1:4976289/76‑1 (MQ=255)
ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGaa < 2:5977559/76‑1 (MQ=255)
ttGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGaa < 2:4475054/76‑1 (MQ=255)
gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAAcc > 2:6511886/1‑76 (MQ=255)
gtgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAAcc > 1:3846587/1‑76 (MQ=255)
tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCt > 1:688144/1‑76 (MQ=255)
tgtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCt < 1:23185/76‑1 (MQ=255)
gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTa < 2:1430869/76‑1 (MQ=255)
gtTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTa < 1:1617647/76‑1 (MQ=255)
tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGt > 2:704849/1‑76 (MQ=255)
tAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGt > 2:4940170/1‑76 (MQ=255)
aaCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTg < 2:7265887/76‑1 (MQ=255)
aCTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTgg < 1:3406487/76‑1 (MQ=255)
cTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGc < 1:6333632/76‑1 (MQ=255)
tCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCg < 1:5937238/76‑1 (MQ=255)
tCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCg > 2:3098504/1‑76 (MQ=255)
ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGa > 2:6851169/1‑76 (MQ=255)
ccAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGa > 2:5438913/1‑76 (MQ=255)
cggCGGTTTCAGGCGATGGTCTATAAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGAt < 2:4493563/74‑1 (MQ=255)
cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGAt < 2:5025427/76‑1 (MQ=255)
aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGAtt > 2:5367711/1‑76 (MQ=255)
gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTc < 2:5861954/76‑1 (MQ=255)
cGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTcc < 1:3535933/76‑1 (MQ=255)
gTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCaa < 1:4253725/76‑1 (MQ=255)
tttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAt > 1:1198982/1‑76 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCacat > 2:4782393/1‑73 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 2:2528794/1‑76 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 1:6512849/1‑76 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 2:7320719/1‑76 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAtt > 1:5524890/1‑76 (MQ=255)
tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAAttt < 2:719239/76‑1 (MQ=255)
ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAt < 1:6241513/76‑1 (MQ=255)
ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAt < 2:2293056/76‑1 (MQ=255)
cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAtct < 1:6434211/76‑1 (MQ=255)
cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAtct > 1:5027046/1‑76 (MQ=255)
gATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCAtctc < 1:1059855/76‑1 (MQ=255)
tGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCt > 2:7670333/1‑76 (MQ=255)
ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt > 2:2332783/1‑76 (MQ=255)
ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt < 1:1426675/76‑1 (MQ=255)
ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt < 2:3576414/76‑1 (MQ=255)
ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCtt > 2:5753997/1‑76 (MQ=255)
gTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTta > 1:4370633/1‑76 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTTCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat < 1:5057065/76‑1 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat > 2:560759/1‑76 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTtat > 2:2918962/1‑76 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa < 2:169166/76‑1 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa > 2:4657637/1‑76 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATaa < 1:3213810/76‑1 (MQ=255)
aTCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAt > 2:6144410/1‑76 (MQ=255)
aTCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAt > 2:1065177/1‑76 (MQ=255)
tCAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAAtt < 1:772262/76‑1 (MQ=255)
cAGAGCTCGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa < 2:5188923/76‑1 (MQ=255)
cAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa > 2:6339803/1‑76 (MQ=255)
cAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa < 1:6339475/76‑1 (MQ=255)
cAGAGCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTa < 2:1627671/76‑1 (MQ=255)
agagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAg > 1:817295/1‑76 (MQ=255)
agagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAg > 1:1241440/1‑76 (MQ=255)
gagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc < 2:3376341/76‑1 (MQ=255)
gagCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGc > 2:6232677/1‑76 (MQ=255)
gCACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCtt < 2:1001695/76‑1 (MQ=255)
cACGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCCATTTCATCTCCTTATAATTAGCTTg > 2:1953654/1‑76 (MQ=255)
cGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt < 2:6484330/76‑1 (MQ=255)
cGCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCt > 2:5713715/1‑76 (MQ=255)
gCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa > 1:188439/1‑76 (MQ=255)
gCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 1:5815414/76‑1 (MQ=255)
gCCATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 2:1987567/76‑1 (MQ=255)
ccATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCGATTTCATCTCCTTATAATTAGCTTGCTaa > 2:3801291/1‑76 (MQ=255)
ccATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTaa < 1:6403505/76‑1 (MQ=255)
cATATGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg < 2:4343552/76‑1 (MQ=255)
atatGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGa < 2:3640815/76‑1 (MQ=255)
tatGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 1:7087518/76‑1 (MQ=255)
atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 2:2629233/76‑1 (MQ=255)
atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata > 1:3055485/1‑76 (MQ=255)
atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 2:6986994/76‑1 (MQ=255)
atGCGCACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 1:4732029/76‑1 (MQ=255)
gcgcACCAACCGTTCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 1:522268/76‑1 (MQ=255)
gcgcACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 1:4279860/76‑1 (MQ=255)
cgcTCCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta < 1:5848980/76‑1 (MQ=255)
cACCAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg < 1:5293918/76‑1 (MQ=255)
ccAACCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg < 1:7320719/76‑1 (MQ=255)
aaCCGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc > 1:4557774/1‑76 (MQ=255)
ccGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt < 1:83365/76‑1 (MQ=255)
ccGTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt > 1:4942261/1‑76 (MQ=255)
gTGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt > 2:3188651/1‑76 (MQ=255)
ttccAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 2:4197818/74‑1 (MQ=255)
tGCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 1:256580/76‑1 (MQ=255)
gCCAGAGCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg > 1:7365138/1‑76 (MQ=255)
agagCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt > 2:3913447/1‑76 (MQ=255)
|
TGGGTTTGTGTTAACTCCAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAT > REL606/1697921‑1698068
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
