Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A131 F1 I1 R1
|
13 |
244.3 |
15300636 |
95.6% |
14627408 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,698,011 |
G→A |
S3L (TCG→TTG) |
slyA ← |
transcriptional regulator SlyA |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,698,011 | 0 | G | A | 98.8%
| 1045.1
/ 1.1
| 247 | S3L (TCG→TTG) | slyA | transcriptional regulator SlyA |
| Reads supporting (aligned to +/- strand): ref base G (2/1); new base A (89/155); total (91/156) |
| Fisher's exact test for biased strand distribution p-value = 5.57e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01 |
Only 100 of 248 total aligned reads displayed.
CAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTG > REL606/1697938‑1698083
|
cAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAAt < 2:1993347/76‑1 (MQ=255)
aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAAtt > 2:4425680/1‑76 (MQ=255)
aGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAAtt < 1:4248067/76‑1 (MQ=255)
gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTc < 1:6052980/76‑1 (MQ=255)
gCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTc < 2:431459/76‑1 (MQ=255)
ggTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCa < 1:2227496/76‑1 (MQ=255)
ggTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCa < 1:2880179/76‑1 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAtt > 1:1640909/1‑76 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAtt < 1:2163321/76‑1 (MQ=255)
ttCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAtt > 1:4699724/1‑76 (MQ=255)
tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAttt < 2:333923/76‑1 (MQ=255)
tCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAAttt > 1:2074078/1‑76 (MQ=255)
ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAt < 1:5262896/76‑1 (MQ=255)
ggCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAt < 2:3651974/76‑1 (MQ=255)
gCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtc < 1:6092357/76‑1 (MQ=255)
gCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtc < 2:4243731/76‑1 (MQ=255)
cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtct > 2:3705573/1‑76 (MQ=255)
cGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCAtct > 2:68675/1‑76 (MQ=255)
aTGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTcc > 2:3838898/1‑76 (MQ=255)
ggTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCtt > 1:5665091/1‑76 (MQ=255)
gTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTta < 2:7598444/76‑1 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTTCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat < 1:3175333/76‑1 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat < 2:1033044/76‑1 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat > 2:2373228/1‑76 (MQ=255)
tCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtat < 1:5115841/76‑1 (MQ=255)
cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtata < 1:2953261/76‑1 (MQ=255)
cTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTtata > 1:1405440/1‑76 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa < 2:1483133/76‑1 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa < 2:7519301/76‑1 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa > 2:1942328/1‑76 (MQ=255)
tATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATaa < 1:4580744/76‑1 (MQ=255)
tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAAtt < 2:5210412/76‑1 (MQ=255)
tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAAtt > 2:6331340/1‑76 (MQ=255)
tCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAAtt < 1:120226/76‑1 (MQ=255)
cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTa > 1:7520480/1‑76 (MQ=255)
cAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTa > 2:2585132/1‑76 (MQ=255)
agagCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAg < 1:3940932/76‑1 (MQ=255)
agCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCt < 1:645446/76‑1 (MQ=255)
gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 2:1372756/76‑1 (MQ=255)
gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 1:3465691/76‑1 (MQ=255)
gCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTa < 1:7362585/76‑1 (MQ=255)
ccATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTaa < 1:3092919/76‑1 (MQ=255)
cATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAg > 2:2826064/1‑76 (MQ=255)
tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 1:7092727/76‑1 (MQ=255)
tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 2:2245299/76‑1 (MQ=255)
tatGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGat < 1:3788368/76‑1 (MQ=255)
atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata > 1:1261293/1‑76 (MQ=255)
atGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGata < 1:4647202/76‑1 (MQ=255)
tGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGatat < 2:487312/76‑1 (MQ=255)
gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 2:821217/76‑1 (MQ=255)
gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 2:5132201/76‑1 (MQ=255)
gcgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatt < 2:5396148/76‑1 (MQ=255)
cgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta > 2:6757233/1‑76 (MQ=255)
cgcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtatta < 1:80889/76‑1 (MQ=255)
gcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtattat < 1:1687283/76‑1 (MQ=255)
gcACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGAtattat < 2:6274985/76‑1 (MQ=255)
cACCAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATg > 2:3191647/1‑76 (MQ=255)
ccAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCg > 1:6983056/1‑76 (MQ=255)
cAACCGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCgg > 2:4151580/1‑76 (MQ=255)
aaCCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGc > 2:6482737/1‑76 (MQ=255)
ccGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtt < 1:4877554/76‑1 (MQ=255)
cGTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCttt < 1:6583924/76‑1 (MQ=255)
gTGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCtttt < 2:911017/76‑1 (MQ=255)
tGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 2:5529711/76‑1 (MQ=255)
tGCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTa < 2:2810074/76‑1 (MQ=255)
gCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg < 1:2479727/76‑1 (MQ=255)
gCCAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAg < 1:7382353/76‑1 (MQ=255)
cAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa < 2:1846694/76‑1 (MQ=255)
cAGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGaa < 2:608152/76‑1 (MQ=255)
aGATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAAt < 1:2101164/76‑1 (MQ=255)
gATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa < 1:5018581/76‑1 (MQ=255)
gATCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATa < 2:4697207/76‑1 (MQ=255)
aTCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg < 1:432378/76‑1 (MQ=255)
aTCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAg < 2:3979230/76‑1 (MQ=255)
tCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgt < 1:4108860/76‑1 (MQ=255)
tCAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgt > 2:2188508/1‑76 (MQ=255)
cAGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtg > 2:2148755/1‑76 (MQ=255)
aGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt < 1:350007/76‑1 (MQ=255)
aGAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAgtgt > 2:7085306/1‑76 (MQ=255)
gAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg < 2:4567321/76‑1 (MQ=255)
gAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg < 2:5759020/76‑1 (MQ=255)
gAACCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtg > 2:2736889/1‑76 (MQ=255)
aaCCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGtgtgg > 2:6031117/1‑75 (MQ=255)
aaCCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgc > 1:4162649/1‑76 (MQ=255)
aCCTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgca < 1:2608621/76‑1 (MQ=255)
ccTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag < 2:4472914/76‑1 (MQ=255)
ccTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag > 1:327278/1‑76 (MQ=255)
ccTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcag > 2:1738487/1‑76 (MQ=255)
cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc > 1:2807106/1‑76 (MQ=255)
cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc > 2:5696557/1‑76 (MQ=255)
cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc < 1:549368/76‑1 (MQ=255)
cTAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagc > 2:1153555/1‑76 (MQ=255)
tAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca < 1:1174256/76‑1 (MQ=255)
tAGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTgcagca < 1:419300/76‑1 (MQ=255)
tgTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa > 2:6599894/2‑76 (MQ=255)
aGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa > 2:4858620/1‑76 (MQ=255)
aGTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCaa < 2:7170261/76‑1 (MQ=255)
gTGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAt < 2:125031/76‑1 (MQ=255)
tGGCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAAtt < 1:1101083/76‑1 (MQ=255)
ggCAATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTg > 1:4900638/1‑76 (MQ=255)
|
CAGCGGTTTCAGGCGATGGTCTATCAGAGCACGCCATATGCGCACCAACCGTGCCAGATCAGAACCTAGTGGCGATTCCAATTTCATCTCCTTATAATTAGCTTGCTAAGATATTATGCGGCTTTTAGAATAGTGTGCAGCAATTG > REL606/1697938‑1698083
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
