Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F1 I1 R1
|
7 |
175.1 |
22577466 |
95.2% |
21493747 |
37.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
REL606 |
1,733,297 |
(CGTATGAA)1→2 |
coding (333/1413 nt) |
pykF → |
pyruvate kinase |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
REL606 |
1733288 = | 44 (0.260) | 167 (1.060) |
62/68 |
0.1 |
83.4% |
coding (324/1413 nt) |
pykF |
pyruvate kinase |
| ? | REL606 |
= 1733295 |
25 (0.160) | coding (331/1413 nt) |
pykF |
pyruvate kinase |
Only 100 of 184 total aligned reads displayed.
TGCCAACAGACAGGTCAGTAGTGAAACCTTCATACGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < REL606/1733325‑1733288
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttCATACGTTACCGCAACCATTTCGCTGTTGCCGATAA < REL606/1733295‑1733260
TGCCAACAGACAGGTCAGTAGTGAAACCTTCATACGT > 1:10158184/1‑37
TGCCAACAGACAGGTCAGTAGTGAAACCTTCATACGT > 2:3419192/1‑37
TGCCAACAGACAGGTCAGTAGTGAAACCTTCATACGT < 2:6232753/37‑1
GCCAACAGACAGGTCAGTAGTGAAACCTTCATACGTT < 1:2145993/37‑1
GCCAACAGACAGGTCAGTAGTGAAACCTTCATACGTT < 1:3111984/37‑1
CCAACAGACAGGTCAGTAGTGAAACCTTCATACGTTC > 1:7091124/1‑37
CCAACAGACAGGTCAGTAGTGAAACCTTCATACGTTC > 1:314251/1‑37
CAACAGACAGGTCAGTAGTGAAACCTTCATACGTTCA > 1:7415070/1‑37
CAACAGACAGGTCAGTAGTGAAACCTTCATACGTTCA > 1:7840657/1‑37
CAACAGACAGGTCAGTAGTGAAACCTTCATACGTTCA > 2:8354281/1‑37
CAACAGACAGGTCAGTAGGGAAACCTTCATAAGTTCA > 2:5711624/1‑37
AACAGACAGGTCAGTAGTGAAACCTTCATACGTTCAT < 2:2231288/37‑1
AACAGACAGGTCAGTAGTGAAACCTTCATACGTTCAT < 1:4656034/37‑1
AACAGACAGGTCAGTAGTGAAACCTTCATACGTTCAT < 1:10139953/37‑1
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA < 1:2988229/37‑1
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA < 2:11027136/37‑1
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA < 2:10177934/37‑1
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA > 2:4860502/1‑37
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA < 2:4999958/37‑1
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA > 1:5520800/1‑37
ACAGACAGGTCAGTAGTGAAACCTTCATACGTTCATA > 2:520126/1‑37
ACAGACAGGGCAGTAGTGAAACCTTCATACGTTCATA > 1:10281593/1‑37
CAGACAGGTCAGTAGTGAAACCTTCATACGTTCATAC < 1:3824335/37‑1
CAGACAGGTCAGTAGTGAAACCTTCATACGTTCATAC > 2:2060781/1‑37
CAGACAGGTCAGTAGTGAAACCTTCATACGTTCATAC < 1:7997331/37‑1
AGACAGGTCAGTAGTGAAACCTTCATACGTTCATACG > 1:7780818/1‑37
GACAGGTCAGTAGTGAAACCTTCATACGTTCATACGT > 2:4571714/1‑37
ACAGGTCAGTAGTGAAACCTTCATACGTTCATACGTT < 1:994375/37‑1
ACAGGTCAGTAGTGAAACCTTCATACGTTCATACGTT > 1:9198733/1‑37
ACAGGTCAGTAGTGAAACCTTCATACGTTCATACGTT < 1:4804349/37‑1
ACAGGTCAGTAGTGAAACCTTCATACGTTCATACGTT < 1:619465/37‑1
CAGGTCAGTCGTGAAACCTTCATACGTTCATACGTTA < 1:558380/37‑1
CAGGTCAGTAGTGAAACCTTCATACGTTCATACGTTA > 2:10057260/1‑37
AGGTCAGTAGTGAAACCTTCATACGTTCATACGTTAC < 1:4417791/37‑1
AGGTCAGTAGTGAAACCTTCATACGTTCATACGTTAC > 1:8444204/1‑37
AGGTCAGTAGTGAAACCTTCATACGTTCATACGTTAC > 2:1573589/1‑37
AGGTCAGTAGTGAAACCTTCATACGTTCATACGCTAC > 2:6758301/1‑37
GGTCAGTAGTGAAACCTTCATACGTTCATACGTTACC > 2:3394677/1‑37
GGTCAGTAGTGAAACCTTCATACGTTCATACGTTACC > 2:10970601/1‑37
GGTCAGTAGTGAAACCTTCATACGTTCATACGTTACC < 1:8802019/37‑1
GGTCAGTAGTGAAACCTTCATACGTTCATACGTTACC > 1:8177997/1‑37
GGTCAGTAGTGAAACCTTCATACGTTCATACGTTACC > 1:114275/1‑37
GTCAGTAGTGAAACCTTCATACGTTCATACGTTACCG > 2:3815429/1‑37
GTCAGTAGTGAAACCTTAATACGTTCATACGTTACCG > 1:3588490/1‑37
TCAGTAGTGAAACCTTCATACGTTCATACGTTACCGC > 2:4582642/1‑37
TCAGTAGTGAAACCTTCATACGTTCATACGTTACCGC < 1:2424569/37‑1
TCAGTAGTGAAACCTTCATACGTTCATACGTTACCGC > 2:2191455/1‑37
TCAGTAGTGAAACCTTCATACGTTCATACGTTACCGC > 1:4572057/1‑37
CAGTAGTGAAACCTTCATACGTTCATACGTTACCGCA > 2:7048508/1‑37
CAGTAGTGAAACCTTCATACGTTCATACGTTACCGCA > 2:10481213/1‑37
CAGTAGTGAAACCTTCATACGTTCATACGTTACCGCA < 1:4459071/37‑1
GTAGTTAAACCTTCATACGTTCCTACGTTCCCGCAAC < 1:9656832/37‑1
GTAGTGAAACCTTCATACGTTCATACGTTACCGCAAC < 2:4404044/37‑1
AGTGAAACCTTCATACGTTCATACGTTACCGCAACCA < 2:8532045/37‑1
AGTGAAACCTTCATACGTTCATACGTTACCGCAACCA < 1:9447505/37‑1
TGAAACCTTCATACGTTCATACGTTACCGCAACCATT < 2:5520566/37‑1
GAAACCTTCATACGTTCATACGTTACCGCAACCATTT > 1:1289333/1‑37
AAACCTTCATACGTTCATACGTTACCGCAACCATTTC > 2:4707994/1‑37
AAACCTTCATACGTTCATACGTTACCGCAACCATTTC < 1:8915953/37‑1
AAACCTTCATACGTTCATACGTTACCGCAACCATTTC > 2:8126965/1‑37
AAACCTTCATACGTTCATACGTTACCGCAACCATTTC < 2:3267398/37‑1
AACCTTCATACGTTCATACGTTACCGCAACCATTTCG < 2:6904817/37‑1
AACCTTCATACGTTCATACGTTACCGCAACCATTTCG < 2:3596166/37‑1
AACCTCCATACTTTCATACGTTACCGCACCCATTTCG < 1:9982849/37‑1
CCTTCATACGTTCATACGTTACCGCAACCATTTCGCT < 2:7630302/37‑1
CCTTCATACGTTCATACGTTACCGCAACCATTTCGCT > 2:2527260/1‑37
CTTCATACGTTCATACGTTACCGCAACCATTTCGCTG < 2:9879965/37‑1
CTTCATACGTTCATACGTTACCGCAACCATTTCGCTG > 1:6323871/1‑37
CTTCATACGTTCATACGTTACCGCAACCATTTCGCTG < 1:6836907/37‑1
CTTCATACGTTCATACGTTACCGCAACCATTTCGCTG > 1:4325047/1‑37
TTCATACGTTCATACGTTACCGCAACCATTTCGCTGT < 1:6601678/37‑1
TTCATACGTTCATACGTTACCGCAACCATTTCGCTGT < 1:6987572/37‑1
TCATACGTTCATACGTTACCGCAACCATTTCGCTGTT > 2:413987/1‑37
TCATACGTTCATACGTTACCGCAACCATTTCGCTGTT < 1:9039265/37‑1
TCATACGTTCATACGTTACCGCAACCATTTCGCTGTT > 2:2994915/1‑37
CATACGTTCATACGTTACCGCAACCATTTCTCTGTTG < 2:1934179/37‑1
CATACGTTCATACGTTACCGCAACCATTTCGCTGTTG > 1:5088783/1‑37
ATACGTTCATACGTTACCGCCACCATTTCGCTGTTGC > 1:5311400/1‑37
ATACGTTCATACGTTACCGCAACCATTTCGCTGTTGC > 2:1458690/1‑37
ATACGTTCATACGTTACCGCAACCATTTCGCTGTTGC < 2:5267228/37‑1
TACGTTCATACGTTACCTCAACCATTTCGCTGTTGCC < 1:249950/37‑1
TACGTTCATACGTTACCGCAACCATTTCGCTGTTGCC < 1:693371/37‑1
TACGTTCATACGTTACCGCAACCATTTCGCTGTTGCC < 1:2858591/37‑1
TACGTTCATACGTTACCGCAACCATTTCGCTGTTGCC > 2:1165708/1‑37
TACGTTCATACGTTACCGCAACCATTTCGCTGTTGCC < 1:2110213/37‑1
TACGTTCATACGTTACCGCAACCATTTCGCTGTTGCC < 1:891371/37‑1
ACGTTCATACGTTACCGCAACCATTTCGCTGTTGCCG > 2:555802/1‑37
CGTTCATACGTTACCGCAACCATTTCGCTTTTGCCGA < 1:9005068/37‑1
CGTTCATACGTTACCGCAACCATTTCGCTGTTGCCGA < 1:7155245/37‑1
GTTCATACGTTCCCGCAACCATTTCGCTGTTGCCGAT > 2:7145876/1‑37
GTTCATACGTTACCGCAACCATTTCGCTGTTGCCGAT < 2:6889966/37‑1
GTTCATACGTTACCGCAACCATTTCGCTGTTGCCGAT < 2:7334823/37‑1
GTTCATACGTTACCGCAACCATTTCGCTGTTGCCGAT > 2:3982839/1‑37
GTTCATACGTTACCGCAACCATTTCGCTGTTGCCGAT < 1:10999226/37‑1
GTTCATACGTTACCGCAACCATTTCGCTGTTGCCGAT > 2:3513104/1‑37
TTCATACGTTCCCGCGACCATTTCGCTGTTGCCGATA < 1:4269565/37‑1
TTCATACGTTACCGCAACCATTTCGCTGTTGCCGATA < 2:8788273/37‑1
TTCATACGTTACCGCAACCATTTCGCTGTTGCCGATA > 2:9630139/1‑37
TCATACGTTACCGCAACCATTTCGCTGTTGCCGATAA > 1:7533943/1‑37
TCATACGTTACCGCAACCATTTCGCTGTTGCCGATAA < 2:9530384/37‑1
TGCCAACAGACAGGTCAGTAGTGAAACCTTCATACGTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ < REL606/1733325‑1733288
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ttCATACGTTACCGCAACCATTTCGCTGTTGCCGATAA < REL606/1733295‑1733260
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
N/A
