Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A80 F1 I1 R1
|
17 |
48.4 |
3161007 |
95.9% |
3031405 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| JC |
REL606 |
1,733,297 |
(CGTATGAA)1→2 |
coding (333/1413 nt) |
pykF → |
pyruvate kinase |
|
| |
seq id |
position |
reads (cov) |
reads (cov) |
score |
skew |
freq |
annotation |
gene |
product |
| * |
? |
REL606 |
= 1733287 | 0 (0.000) | 37 (0.850)
+AACGTATG |
28/114 |
0.2 |
100% |
coding (323/1413 nt) |
pykF |
pyruvate kinase |
| ? | REL606 |
1733288 = |
0 (0.000) | coding (324/1413 nt) |
pykF |
pyruvate kinase |
CTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/1733215‑1733287
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAT > REL606/1733288‑1733359
||||||||
CTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAAC < 2:208109/76‑1
TGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTA < 1:1399800/76‑1
TGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTA < 1:295268/76‑1
GAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTAT > 2:592426/1‑76
AAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATG > 2:593530/1‑76
AAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGA < 2:571911/76‑1
GGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTA < 1:1122350/76‑1
GTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTAT < 1:1426326/76‑1
TCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATG > 2:383691/1‑76
TCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATG > 2:190281/1‑76
CAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGA < 2:616196/76‑1
CAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGA > 2:524919/1‑76
TTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTT < 2:908895/76‑1
TTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTC > 1:1566532/1‑76
TACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCA < 1:795361/76‑1
ACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAAAGTATGAACGTATGAAGGTTTCAC > 1:1209135/1‑76
TTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTAC > 2:1376866/1‑76
TCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACT < 1:1380320/76‑1
TCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACT > 2:268785/1‑76
CACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTG < 2:1538635/76‑1
CACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTG > 1:881256/1‑76
ACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGA < 1:1246452/76‑1
ACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGA < 1:108442/76‑1
CTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTCTCACTACTGACCTG > 1:1529847/1‑76
GATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTC > 1:1424641/1‑76
GATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTC < 1:524100/76‑1
GATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTC > 1:457563/1‑76
ATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCT < 2:1193305/76‑1
ATCTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTG > 1:1537680/1‑76
CTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGC > 1:108212/1‑76
CTGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGC > 1:407852/1‑76
TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA < 1:310862/76‑1
TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA > 1:507414/1‑76
TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA < 2:108002/76‑1
TGTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCA > 1:967227/1‑76
GTTATCGGCAACAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAA > 1:505032/1‑76
CAGCGAAATGGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGGCCTGTCTGTTGGCAACACCGTACTGG < 1:605907/76‑1
GGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATG < 2:21326/76‑1
GGTTGCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATG < 2:470942/76‑1
GCGGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCT < 2:751767/76‑1
GGTAACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGA < 1:778218/76‑1
AACGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCG > 2:701277/1‑76
CGTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGT > 2:1386321/1‑76
GTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTA > 2:1408073/1‑76
GTATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTA > 1:13699/1‑76
TATGAACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAT > 1:438768/1‑76
||||||||
CTCTGAAAGCTGGTCAGACCTTTACTTTCACCACTGATAAATCTGTTATCGGCAACAGCGAAATGGTTGCGGT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > REL606/1733215‑1733287
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑AACGTATGAAGGTTTCACTACTGACCTGTCTGTTGGCAACACCGTACTGGTTGACGATGGTCTGATCGGTAT > REL606/1733288‑1733359
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
|---|
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |
GATK/CNVnator alignment
N/A
