Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A119 F1 I1 R1
|
20 |
247.5 |
15984500 |
92.7% |
14817631 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,651 |
C→T |
V110I (GTT→ATT) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,651 | 0 | C | T | 98.7%
| 949.5
/ 1.4
| 234 | V110I (GTT→ATT) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base C (2/1); new base T (119/112); total (121/113) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.36e-01 |
Only 100 of 245 total aligned reads displayed.
CCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAC > REL606/3328578‑3328723
|
ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgc > 2:5027332/1‑76 (MQ=255)
ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgc > 2:6852410/1‑76 (MQ=255)
ccAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgc > 2:3891762/1‑76 (MQ=255)
aGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgcgc > 1:4035717/1‑76 (MQ=255)
aGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgcgc > 2:2761188/1‑76 (MQ=255)
gcgcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAAtgcgcg < 2:7307086/76‑1 (MQ=255)
cgcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCgg < 1:1383937/76‑1 (MQ=255)
gcCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCggg > 2:4401214/1‑76 (MQ=255)
cccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGc > 1:3335627/1‑76 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCt < 2:732880/76‑1 (MQ=255)
ccTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCt > 1:5323373/1‑76 (MQ=255)
gcgcGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTgg < 1:5752415/76‑1 (MQ=255)
cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGa > 2:1422963/1‑76 (MQ=255)
cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGa > 1:805012/1‑76 (MQ=255)
cgcgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGa < 1:3187723/76‑1 (MQ=255)
cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg > 2:7089978/1‑76 (MQ=255)
cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg > 2:7727160/1‑76 (MQ=255)
cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg < 2:802740/76‑1 (MQ=255)
cgGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACg < 1:40801/76‑1 (MQ=255)
ggATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGc > 1:879106/1‑76 (MQ=255)
ggATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGc > 2:6901493/1‑76 (MQ=255)
gATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCa > 1:7224071/1‑76 (MQ=255)
gATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCa > 2:3823857/1‑76 (MQ=255)
aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCAt < 1:7918176/76‑1 (MQ=255)
aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCAt > 1:6578439/1‑76 (MQ=255)
aTTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCAt > 1:376585/1‑76 (MQ=255)
ttCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGAATa > 2:1638499/1‑76 (MQ=255)
tCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaa < 1:3937942/76‑1 (MQ=255)
cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa > 1:1994531/1‑76 (MQ=255)
cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 1:1973471/76‑1 (MQ=255)
cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 1:2981416/76‑1 (MQ=255)
cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 1:681139/76‑1 (MQ=255)
cACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATaaa < 2:5340818/76‑1 (MQ=255)
aCGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAg > 2:1374126/1‑76 (MQ=255)
aaTTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTg > 1:5810986/1‑76 (MQ=255)
aTTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGt > 2:3576127/1‑76 (MQ=255)
tGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTg < 2:461746/76‑1 (MQ=255)
cggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCtgt > 1:6349145/1‑76 (MQ=255)
cggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCtgt > 2:3603767/1‑76 (MQ=255)
ggcgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCtgtg < 2:5883666/76‑1 (MQ=255)
cgTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCa < 1:260344/76‑1 (MQ=255)
gTTCAACCTGGTTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCAc < 1:1069840/76‑1 (MQ=255)
gTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCAc > 1:4958174/1‑76 (MQ=255)
ttCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACt > 1:7654324/1‑76 (MQ=255)
ttCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACt > 1:4359714/1‑76 (MQ=255)
aaCCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt > 2:6331861/1‑76 (MQ=255)
aaCCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt > 2:2946576/1‑76 (MQ=255)
aaCCTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGt > 2:4540675/1‑76 (MQ=255)
ccTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt < 1:646989/76‑1 (MQ=255)
ccTGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGttt < 2:219437/76‑1 (MQ=255)
cTGGGTCACGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtg < 1:4281955/76‑1 (MQ=255)
tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga > 1:2764882/1‑76 (MQ=255)
tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga < 1:1580486/76‑1 (MQ=255)
tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga > 2:1854335/1‑76 (MQ=255)
tGGGTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtga < 1:757508/76‑1 (MQ=255)
ggTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 2:4899956/1‑76 (MQ=255)
ggTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatg > 2:3501410/1‑76 (MQ=255)
gTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatga < 1:526311/76‑1 (MQ=255)
gTCGCGCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTtgatga > 2:6789687/1‑76 (MQ=255)
cgcgCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg < 1:4389987/76‑1 (MQ=255)
cgcgCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg < 2:307522/76‑1 (MQ=255)
cgcgCCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAg > 2:2315283/1‑76 (MQ=255)
cgcCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg > 2:7600227/1‑76 (MQ=255)
cgcCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg < 2:5763037/76‑1 (MQ=255)
cgcCAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTg < 2:7874525/76‑1 (MQ=255)
ccAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCt > 1:128559/1‑76 (MQ=255)
cAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTg < 2:5651880/76‑1 (MQ=255)
cAACCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTg < 2:2071653/76‑1 (MQ=255)
aaCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTgg < 2:5117052/76‑1 (MQ=255)
aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa > 1:7431533/1‑76 (MQ=255)
aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:6275066/76‑1 (MQ=255)
aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:5897271/76‑1 (MQ=255)
aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:464522/76‑1 (MQ=255)
aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 1:5116589/76‑1 (MQ=255)
aCCGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGa < 2:313244/76‑1 (MQ=255)
ccGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg < 2:7466503/76‑1 (MQ=255)
ccGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg < 1:2875543/76‑1 (MQ=255)
ccGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAg < 2:5503885/76‑1 (MQ=255)
cGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc > 2:3982333/1‑76 (MQ=255)
cGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc < 1:2851080/76‑1 (MQ=255)
cGGCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGc > 2:5602843/1‑76 (MQ=255)
ggCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCa < 1:7153396/76‑1 (MQ=255)
gCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt < 2:1185419/76‑1 (MQ=255)
gCACACAAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAt > 2:2664886/1‑76 (MQ=255)
cacaAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttt > 2:4708550/1‑76 (MQ=255)
acaAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAttttt > 1:1089611/1‑76 (MQ=255)
caAACCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCAtttttt < 1:4201426/76‑1 (MQ=255)
aaaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTc > 2:6228160/1‑76 (MQ=255)
aaaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTc > 2:2609260/1‑76 (MQ=255)
aaaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTc < 1:6221182/76‑1 (MQ=255)
aaCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCa < 1:348322/76‑1 (MQ=255)
aCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg > 2:6117101/1‑76 (MQ=255)
aCCAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAg < 2:7438202/76‑1 (MQ=255)
ccAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGt > 1:609245/1‑76 (MQ=255)
cAGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTc < 1:7400827/76‑1 (MQ=255)
aGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCa > 1:7794609/1‑76 (MQ=255)
aGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCa > 1:5448330/1‑76 (MQ=255)
aGAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCa > 2:5624623/1‑76 (MQ=255)
gAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAc > 2:1174698/1‑76 (MQ=255)
gAATGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAc < 2:495301/76‑1 (MQ=255)
|
CCAGCGCCCTGCGCGGATTCACGAATTGCGCGGCGTTCAACCTGGGTCGCGCCAACCGGCACACAAACCAGAACGCGCGGGCTTGGACGCATAAAGCTGTTGCTGTGCACTTGTTTGATGAAGTGCTGGAGCATTTTTTCAGTCAC > REL606/3328578‑3328723
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
