Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A54 F1 I1 R1
|
16 |
53.7 |
3449390 |
96.4% |
3325211 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,360,241 |
G→A |
V635I (GTT→ATT) |
pta → |
phosphate acetyltransferase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,360,241 | 0 | G | A | 100.0%
| 160.7
/ NA
| 43 | V635I (GTT→ATT) | pta | phosphate acetyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (28/15); total (28/15) |
TCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACA > REL606/2360168‑2360313
|
tCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACAtt < 2:761335/76‑1 (MQ=255)
tGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGc < 2:518445/76‑1 (MQ=255)
gcgcAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGa > 1:1686550/1‑76 (MQ=255)
cAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAAt > 1:874360/1‑76 (MQ=255)
aGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATc > 2:1237718/1‑76 (MQ=255)
aGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATc < 1:433582/76‑1 (MQ=255)
aaaaaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCaa < 1:1135336/76‑1 (MQ=255)
aaaaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCaaa > 2:1657540/1‑76 (MQ=255)
aaaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAg > 2:226584/1‑76 (MQ=255)
aaCGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAgc < 2:1585347/76‑1 (MQ=255)
tCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCg > 2:767777/1‑76 (MQ=255)
cctgacctgaTGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGa > 2:68472/1‑76 (MQ=255)
tgacctgaTGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAAc < 2:290124/76‑1 (MQ=255)
cctgaTGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAActct > 2:1623789/1‑76 (MQ=255)
tgatgaTCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTcc > 2:928983/1‑76 (MQ=255)
tgatgaTCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTcc < 2:199519/76‑1 (MQ=255)
tgatgaTCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTcc > 2:1509390/1‑76 (MQ=255)
gatgatCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCg > 1:778447/1‑76 (MQ=255)
gatCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGtt < 2:908169/76‑1 (MQ=255)
atCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTATCCGGCTg > 2:1051711/1‑76 (MQ=255)
tCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGc > 2:1411297/1‑76 (MQ=255)
cGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCa > 1:402234/1‑76 (MQ=255)
cGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGt > 1:1521285/1‑76 (MQ=255)
ggTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCACGTc > 1:1295289/1‑76 (MQ=255)
tCCGCTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTcgc < 2:591425/76‑1 (MQ=255)
cTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTa > 2:1238136/1‑76 (MQ=255)
cTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTa > 1:736949/1‑76 (MQ=255)
cTGCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTa > 1:540769/1‑76 (MQ=255)
gCAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTAcc > 2:1016672/1‑76 (MQ=255)
cAGTACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGATGCAGGTCGCGCTACCg > 2:337769/1‑76 (MQ=255)
cAGTACGACGCTACGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCg > 1:665991/1‑76 (MQ=255)
tACGACGCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCgtgt > 1:153382/1‑76 (MQ=255)
acgacgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCgtaaa < 2:827404/76‑4 (MQ=255)
cgacgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTc < 2:841277/76‑1 (MQ=255)
acgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCAt < 1:1232917/76‑1 (MQ=255)
acgCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCAt < 1:1004811/76‑1 (MQ=255)
gCTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCt > 1:1254363/1‑76 (MQ=255)
cTGCGGTAATGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCtt > 1:618598/1‑76 (MQ=255)
aaTGGCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGAt > 2:434832/1‑76 (MQ=255)
ggCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTg < 2:1660878/76‑1 (MQ=255)
ggCTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTg < 1:1652983/76‑1 (MQ=255)
gCTGACATTGGTAAATCCAAACCGCCGATCTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGa < 2:965389/76‑1 (MQ=255)
cTGACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGaa > 1:1006657/1‑76 (MQ=255)
gACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAaca > 2:1339362/1‑76 (MQ=255)
gACATTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAaca > 1:1118336/1‑76 (MQ=255)
|
TCTGGCGCAGGAAAAACGTCCTGACCTGATGATCGACGGTCCGCTGCAGTACGACGCTGCGGTAATGGCTGACGTTGCGAAATCCAAAGCGCCGAACTCTCCGGTTGCAGGTCGCGCTACCGTGTTCATCTTCCCGGATCTGAACA > REL606/2360168‑2360313
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
