Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A143 F1 I1 R1
|
16 |
39.8 |
2536279 |
96.2% |
2439900 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,658,199 |
T→G |
L10* (TTA→TGA) |
ydgB → |
short chain dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,658,199 | 0 | T | G | 100.0%
| 204.3
/ NA
| 50 | L10* (TTA→TGA) | ydgB | short chain dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (24/26); total (24/26) |
GTGGGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGAT > REL606/1658126‑1658271
|
gtggGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATatgaa > 1:797781/1‑76 (MQ=255)
tggGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATatgaat > 2:928088/1‑76 (MQ=255)
gggATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATatgaatt > 2:1156235/1‑76 (MQ=255)
aTAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACt > 1:326313/1‑76 (MQ=255)
aTAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACt < 2:612229/76‑1 (MQ=255)
tAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTg > 1:776777/1‑76 (MQ=255)
aaCGTTCATCCCATCGTTGGATTGCNAAGGAGATAACGATGGGTAACNCCCCGCCCTTGCCAATATGAATTACTgg < 1:183228/76‑1 (MQ=255)
gTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCgg > 2:801586/1‑76 (MQ=255)
tCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGgtc < 2:749781/76‑1 (MQ=255)
ccATCGTTGGATAGCAATCGAGATGAGGACAGGTGGAACACGGACCTTACTAATATGCATTAGTGGCGCAGctcgt > 1:1264929/1‑76 (MQ=255)
cATCGTTGNATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGgtggtc > 1:1220205/1‑76 (MQ=255)
cATCGTTGGATAGCAAAGGCGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGggcgtc > 1:1080149/1‑76 (MQ=255)
tCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGc < 2:419714/76‑1 (MQ=255)
cGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCa < 2:228060/76‑1 (MQ=255)
cGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCa < 2:1201971/76‑1 (MQ=255)
gTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCAt < 2:702427/76‑1 (MQ=255)
ttGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATCTGAATTACTGGCGGAGGTCGTCGCATc < 1:1125023/76‑1 (MQ=255)
tGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCg > 1:88317/1‑76 (MQ=255)
tGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTAGTCGCATCg > 1:627468/1‑76 (MQ=255)
ggATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCgg < 1:857907/76‑1 (MQ=255)
ggATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCgg > 1:982704/1‑76 (MQ=255)
aTAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcc > 2:120775/1‑76 (MQ=255)
gCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGGCGCATCGGcctcc > 2:913790/1‑75 (MQ=255)
cAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgc > 2:601235/1‑76 (MQ=255)
aaaGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGTGGTCGTCGCATCGGcctcgcc < 2:899027/76‑1 (MQ=255)
aaaGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgcc > 1:477232/1‑76 (MQ=255)
ggAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctc < 2:692602/76‑1 (MQ=255)
ggAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctc < 1:587372/76‑1 (MQ=255)
ggAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctc < 2:269967/76‑1 (MQ=255)
gagaTAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGcctcgccctcg < 2:239067/76‑1 (MQ=255)
gaTAACGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCa < 1:638426/76‑1 (MQ=255)
aTAACGATGGGTAANACCCAGCCCTTGCCAATATGAATTACTGGCGGGGGTCGTCGCATCGGCCTCGCCCNCCCAt < 2:1266010/76‑1 (MQ=255)
aCGATGGGTAANACNCAGCCCTTGCCTATATGAATTACTGGCGGAGGTCGTCGCATCCTCCTCGCCCTCGCATGGc < 2:195628/76‑1 (MQ=255)
aCGAGGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGc < 1:682967/76‑1 (MQ=255)
cGATGGGTCAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCa > 1:1172854/1‑76 (MQ=255)
cGATGGGTAAAACCCAGCCCTTGCCAATATGAATTACCGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCa > 1:1062567/1‑76 (MQ=255)
aTGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCTTCGCATCGGCCTCGCCCTCGCATGGCAtt < 2:540861/76‑1 (MQ=255)
tGGGTAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCAttt > 2:824062/1‑76 (MQ=255)
tAAAACCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCCTGGCATTTCAtt > 1:1005419/1‑76 (MQ=255)
aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa < 2:913735/76‑1 (MQ=255)
aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa < 2:913750/76‑1 (MQ=255)
aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa > 2:1076787/1‑76 (MQ=255)
aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa < 1:530720/76‑1 (MQ=255)
aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTa < 1:786142/76‑1 (MQ=255)
aaaaCCCAGCCCTTGCCAATATGAATTACTGGCGCAGGTCGTCGCCGCGGCCACGCCCTCGCATTGCGTGTCAtca > 2:1076781/1‑74 (MQ=255)
cccAGCCCTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCa < 1:210815/76‑1 (MQ=255)
ccAGCCCTTGCCAATATGAATTACTGGCGGAGGTAGTCGCANCGGCCTCGCCCTCGCCCGNGANTTCATTAATCaa > 1:1032253/1‑76 (MQ=255)
ccTTGGCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCa > 1:81336/1‑76 (MQ=255)
ccTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCa > 1:213816/1‑76 (MQ=255)
cTTGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCaa > 2:1177890/1‑76 (MQ=255)
tGCCAATATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAAcc < 2:213968/76‑1 (MQ=255)
aaTATGAATTACTGGCGGAGTTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTTATCAAAAGCAACCGGTg < 1:1253188/76‑1 (MQ=255)
aaTATGAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTg > 1:1025936/1‑76 (MQ=255)
tatGAATTACTGGCGGAGGTCGTCGCATCGGCCTAGCNCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGAt > 1:1232336/1‑76 (MQ=255)
|
GTGGGATAACGTTCATCCCATCGTTGGATAGCAAAGGAGATAACGATGGGTAAAACCCAGCCCTTGCCAATATTAATTACTGGCGGAGGTCGTCGCATCGGCCTCGCCCTCGCATGGCATTTCATTAATCAAAAGCAACCGGTGAT > REL606/1658126‑1658271
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
