Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A142 F1 I1 R1
|
21 |
42.6 |
2691336 |
96.3% |
2591756 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
1,658,770 |
T→G |
N200K (AAT→AAG) |
ydgB → |
short chain dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 1,658,770 | 0 | T | G | 95.6%
| 170.1
/ ‑4.3
| 46 | N200K (AAT→AAG) | ydgB | short chain dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base T (1/1); new base G (26/17); total (27/19) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGC > REL606/1658700‑1658842
|
aTTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATc < 2:606750/76‑1 (MQ=255)
ttCTATTGCGCCATNGCTGATCCTGTTTAATGAACATGATGATGCCGTATATCGACAACAGGCGCTTAATNACTCa < 2:1343665/76‑1 (MQ=255)
ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCa > 1:620491/1‑76 (MQ=255)
ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCa > 1:121363/1‑76 (MQ=255)
ttCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATACCGACAACAGGCGCTGAATAAATCa > 1:695296/1‑76 (MQ=255)
tCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCAc < 1:256506/76‑1 (MQ=255)
tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtg > 2:1198224/1‑76 (MQ=255)
tATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtg > 2:1198236/1‑76 (MQ=255)
aTTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtga > 2:285565/1‑76 (MQ=255)
aTTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtga < 1:385497/76‑1 (MQ=255)
ttGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtgat > 1:1219810/1‑76 (MQ=255)
tGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACtgatg > 2:179161/1‑76 (MQ=255)
gcCATCGCTGATCCNGTTTAATGAACATGATGATGCCGAATACCGACAACAGGCGCTGAACAACTCACTGATGaaa < 1:1144814/76‑1 (MQ=255)
gcCATCGCTGATACTCTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGNAATCACTGATGaaa > 1:1151914/1‑76 (MQ=255)
tCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACcg > 2:880726/1‑76 (MQ=255)
gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACcgcg < 2:988241/76‑1 (MQ=255)
gCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACcgcg < 1:770030/76‑1 (MQ=255)
cTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCgcgc > 2:576015/1‑76 (MQ=255)
gATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCt < 1:830384/76‑1 (MQ=255)
aTCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTg > 1:1222162/1‑76 (MQ=255)
tCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTgg > 1:756500/1‑76 (MQ=255)
cTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCg < 2:103102/76‑1 (MQ=255)
tGTTTAATGAATATGATGATGCCGAATATCGACAACAGGTGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCga > 1:36704/1‑76 (MQ=255)
tttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCgaga > 2:579533/1‑76 (MQ=255)
ttAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGagaa < 1:701537/76‑1 (MQ=255)
tGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGagaaagaa < 1:452629/76‑1 (MQ=255)
tGAACATGATGATGCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGagaaagaa > 2:1102386/1‑76 (MQ=255)
gatgatgCCGAATATCGACAACAGGCGCTGAAGGAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCg > 1:154917/1‑76 (MQ=255)
gatgatgCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCg > 1:1084082/1‑76 (MQ=255)
tgatgCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGAc > 2:779790/1‑76 (MQ=255)
gCCGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTgg > 2:967570/1‑76 (MQ=255)
ccGAATATCGACAACACGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGt < 1:398831/76‑1 (MQ=255)
cGAATATCGACAACAGGCGCTGAAGAAATCCCTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc < 2:617279/76‑1 (MQ=255)
cGAATATCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTc > 2:808939/1‑76 (MQ=255)
tatCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtt > 2:897677/1‑76 (MQ=255)
taccGACAACAGGCGCTGAAGAAATCACTGATGAAACCCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtt < 1:435594/73‑1 (MQ=255)
atCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAtta > 2:1273472/1‑76 (MQ=255)
tCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttac > 1:550633/1‑76 (MQ=255)
tCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttac < 1:256638/76‑1 (MQ=255)
tCGACAACAGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttac < 2:1000501/76‑1 (MQ=255)
caacaGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttac > 2:362527/1‑76 (MQ=255)
aacaGGCGCTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttacn < 1:837668/76‑2 (MQ=255)
aGGCGCTGAAGAAATCACTTATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGAttacttacttac < 2:395670/76‑1 (MQ=255)
cgcTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAg > 1:357613/1‑76 (MQ=255)
cTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTTGATGACTTACTTACCAGtt < 2:117411/76‑1 (MQ=255)
cTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGtt < 2:178802/76‑1 (MQ=255)
cTGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGtt > 1:795619/1‑76 (MQ=255)
tGAAGAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTg < 1:520467/76‑1 (MQ=255)
gaagaaATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGc < 2:304628/76‑1 (MQ=255)
|
ATTCTATTGCGCCATCGCTGATCCTGTTTAATGAACATGATGATGCCGAATATCGACAACAGGCGCTGAATAAATCACTGATGAAAACCGCGCCTGGCGAGAAAGAAGTGATCGACCTGGTCGATTACTTACTTACCAGTTGC > REL606/1658700‑1658842
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
