Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A70 F1 I1 R1
|
18 |
48.6 |
3152226 |
95.8% |
3019832 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,842,808 |
G→A |
A428V (GCG→GTG) |
dnaA ← |
chromosomal replication initiation protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,842,808 | 0 | G | A | 100.0%
| 106.2
/ NA
| 29 | A428V (GCG→GTG) | dnaA | chromosomal replication initiation protein |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (13/16); total (13/16) |
GTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGA > REL606/3842735‑3842876
|
gTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAAcaca < 1:714107/76‑1 (MQ=255)
ctTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAAt < 1:739877/76‑1 (MQ=255)
tCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAAtct < 2:1266979/76‑1 (MQ=255)
cAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGc < 2:379351/76‑1 (MQ=255)
cAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGc < 2:128918/76‑1 (MQ=255)
aCTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAg > 2:1337725/1‑76 (MQ=255)
aCTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAg > 1:175428/1‑76 (MQ=255)
cTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGa > 2:1002771/1‑76 (MQ=255)
gCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACt > 1:104823/1‑76 (MQ=255)
cTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACtg > 2:1090395/1‑76 (MQ=255)
cGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCCCGGCCACCAAACACATCGCCAATCTCCCGCAGACtggg > 2:443103/1‑74 (MQ=255)
cGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACtgtg > 2:133102/1‑76 (MQ=255)
tCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGtt > 1:209852/1‑76 (MQ=255)
aggcaTGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGctc > 2:1370425/1‑76 (MQ=255)
caTGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCttt < 2:523006/76‑1 (MQ=255)
caTGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCttt < 2:1142414/76‑1 (MQ=255)
aTGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTc < 2:104375/76‑1 (MQ=255)
aTGAAGCACCGTCGTGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTc < 2:378675/76‑1 (MQ=255)
cgtcgtGTGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGcgcc > 1:940646/1‑76 (MQ=255)
gtgtGGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGcgccatcg < 2:668239/76‑1 (MQ=255)
tgtgGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGcgccatcgc > 2:725047/1‑76 (MQ=255)
gtgGTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGcgccatcgcc > 1:1276256/1‑76 (MQ=255)
ggTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGcgccatcgccat > 1:168655/1‑76 (MQ=255)
gTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCgccatcgccatc < 1:1090395/76‑1 (MQ=255)
gTCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCgccatcgccatc < 1:1498660/76‑1 (MQ=255)
tCACGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCt > 2:672727/1‑76 (MQ=255)
aCGGCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTgg < 1:495972/76‑1 (MQ=255)
ggCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGgcg < 2:239396/76‑1 (MQ=255)
ggCCACCAAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGgcg < 2:181054/76‑1 (MQ=255)
ccaccaAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGcgcg < 2:574406/76‑1 (MQ=255)
caccaAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCgg < 1:657424/76‑1 (MQ=255)
cccaAACACATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGa < 2:1241431/75‑1 (MQ=255)
|
GTGGCTCTCTTCACGCAACTGCTCGATCTTACGGCAGGCATGAAGCACCGTCGTGTGGTCACGGCCACCAAACGCATCGCCAATCTCCGGCAGACTGTGGTTAGTCAGCTCTTTCGCCAGCGCCATCGCCATCTGGCGCGGA > REL606/3842735‑3842876
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
