Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A34 F1 I1 R1
|
18 |
56.1 |
3547650 |
96.9% |
3437672 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,162,521 |
G→A |
G556S (GGT→AGT) |
rpoB → |
DNA‑directed RNA polymerase subunit beta |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,162,521 | 0 | G | A | 100.0%
| 157.0
/ NA
| 39 | G556S (GGT→AGT) | rpoB | DNA‑directed RNA polymerase subunit beta |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (27/12); total (27/12) |
CGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGC > REL606/4162448‑4162591
|
cGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGt < 2:1291551/76‑1 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTcg < 1:1401853/76‑1 (MQ=255)
cACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTcg < 1:782746/76‑1 (MQ=255)
cccAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGt < 1:816288/76‑1 (MQ=255)
cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTcc > 1:1573833/1‑76 (MQ=255)
cAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTcc > 2:1059950/1‑76 (MQ=255)
aGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCa > 2:1026462/1‑76 (MQ=255)
cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATc < 1:89055/76‑1 (MQ=255)
cGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATc > 1:1679399/1‑76 (MQ=255)
ggTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCg > 1:1366975/1‑76 (MQ=255)
ggTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCg > 1:737687/1‑76 (MQ=255)
tGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAAc > 2:998575/1‑76 (MQ=255)
cccGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAAcccc < 1:12991/76‑1 (MQ=255)
aaCGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAgg > 2:672169/1‑76 (MQ=255)
cAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGaa > 1:549154/1‑76 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc > 2:1246677/1‑76 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc > 2:456253/1‑76 (MQ=255)
aGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAAc > 1:870586/1‑76 (MQ=255)
gCTTCGAAGTTCGAGACGTACCCCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACAt < 1:1566537/76‑1 (MQ=255)
cTTCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCCGACGGCCCGAACATc > 1:870096/1‑76 (MQ=255)
ttCGAAGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCg > 1:851217/1‑76 (MQ=255)
aGTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTg < 1:1292902/76‑1 (MQ=255)
gTTCGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGa > 2:815350/1‑76 (MQ=255)
ngagaCGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 1:1178250/2‑76 (MQ=255)
cGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 2:822600/1‑76 (MQ=255)
cGAGACGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCa > 1:1638542/1‑76 (MQ=255)
gagaCGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCaa > 2:991815/1‑76 (MQ=255)
gagaCGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCaa < 1:207859/76‑1 (MQ=255)
cGTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActct < 2:277794/76‑1 (MQ=255)
gTACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctc < 2:732986/76‑1 (MQ=255)
tACACCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAActctct > 2:391268/1‑76 (MQ=255)
acacCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg > 2:611544/1‑76 (MQ=255)
acacCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTg > 2:1237747/1‑76 (MQ=255)
acCCGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTc > 1:821930/1‑76 (MQ=255)
ccGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCg < 2:1234033/76‑1 (MQ=255)
cGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt > 1:666870/1‑76 (MQ=255)
cGACTCACTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCgt > 1:750264/1‑76 (MQ=255)
aCTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc > 1:1723610/1‑76 (MQ=255)
aCTACAGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGc > 1:375908/1‑76 (MQ=255)
|
CGCACTCGGCCCAGGCGGTCTGACCCGTGAACGTGCAGGCTTCGAAGTTCGAGACGTACACCCGACTCACTACGGTCGCGTATGTCCAATCGAAACCCCTGAAGGTCCGAACATCGGTCTGATCAACTCTCTGTCCGTGTACGC > REL606/4162448‑4162591
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
