Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A86 F1 I1 R1
|
15 |
50.6 |
3276358 |
95.9% |
3142027 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,328,101 |
A→G |
L293P (CTC→CCC) |
mreB ← |
cell wall structural complex MreBCD, actin‑like component MreB |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,328,101 | 0 | A | G | 100.0%
| 157.6
/ NA
| 42 | L293P (CTC→CCC) | mreB | cell wall structural complex MreBCD, actin‑like component MreB |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/22); total (20/22) |
AACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAC > REL606/3328028‑3328168
|
aacaacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGc > 1:1188042/1‑76 (MQ=255)
caacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCAc > 1:990597/1‑76 (MQ=255)
aacGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCAcc < 2:1271603/76‑1 (MQ=255)
cGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCAt < 2:337252/76‑1 (MQ=255)
aCTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGc < 1:1055647/76‑1 (MQ=255)
cTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGcc > 1:95980/1‑76 (MQ=255)
aTGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCt < 2:903428/76‑1 (MQ=255)
tGCCGGTTTCTTCCATTACCAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTc < 2:1116596/76‑1 (MQ=255)
gCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCg > 2:1402084/1‑76 (MQ=255)
gCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCg > 1:646341/1‑76 (MQ=255)
cgttttCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGga < 2:1331898/73‑1 (MQ=255)
cGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGga < 2:1571301/76‑1 (MQ=255)
ggTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGgag > 2:564238/1‑76 (MQ=255)
gTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGgaga > 2:1444144/1‑76 (MQ=255)
ttcttcCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATg > 1:77734/1‑76 (MQ=255)
tAACAAGCGGTCAAGGTTACGCAGCAGTGCGCCGCCGCCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGcc < 1:1593386/76‑1 (MQ=255)
tAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGcc < 1:260997/76‑1 (MQ=255)
cAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGt > 1:1201841/1‑76 (MQ=255)
aagcGGTCAAGGTTACGCAGCAGTGCGCCACCGCCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGtt < 1:514850/73‑1 (MQ=255)
aaCGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGCAGCCAGTTc > 2:1592298/1‑76 (MQ=255)
aaCGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTc < 1:1404670/76‑1 (MQ=255)
aCGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTcc < 1:620289/76‑1 (MQ=255)
aCGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTcc < 1:418656/76‑1 (MQ=255)
ngTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcgg > 1:672383/2‑76 (MQ=255)
gTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc > 2:610896/1‑76 (MQ=255)
gTCAAGGTTACGCAGCAGTGCCCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggc < 2:586506/76‑1 (MQ=255)
cAAGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCcggcgg < 2:1403852/76‑1 (MQ=255)
aaGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCggg > 1:269378/1‑76 (MQ=255)
aGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc > 2:1182347/1‑76 (MQ=255)
aGGTTACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGc < 1:1475307/76‑1 (MQ=255)
ttACGCAGCAGTGCGCCACCACCGGAGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCaac > 2:1520743/1‑74 (MQ=255)
tACGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCCCTg > 1:1513927/1‑76 (MQ=255)
aCGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGGACTGt > 2:951809/1‑76 (MQ=255)
cGCAGCAGTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGtt > 2:1467031/1‑76 (MQ=255)
cagcagTGCGCCGCCACCGGTGGGCCCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTcc < 1:636962/76‑1 (MQ=255)
cagcagTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTcc < 1:498130/76‑1 (MQ=255)
cagcagTGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACCCTTcc > 2:348860/1‑76 (MQ=255)
gTGCGCCACCCCCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGc < 2:710100/76‑1 (MQ=255)
tGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCTGTGCa > 2:776844/1‑76 (MQ=255)
tGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCa < 1:1397955/76‑1 (MQ=255)
tGCGCCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACCGGTTCCGTGCa > 2:1362740/1‑76 (MQ=255)
cgcCACCACCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAAc < 1:671093/76‑1 (MQ=255)
caccacCGGTGGGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCAt < 2:139238/76‑1 (MQ=255)
cgccGGTGGGCGCCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAc < 2:1332683/74‑1 (MQ=255)
|
AACAACGACTGGAATGCCGGTTTCTTCCATTAACAAACGGTCAAGGTTACGCAGCAGTGCGCCACCACCGGTGAGCACCATGCCGCGCTCGGAGATGTCGGAAGCCAGTTCCGGCGGGCACTGTTCCAGTGCAACCATTAC > REL606/3328028‑3328168
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
