Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A122 F1 I1 R1
|
27 |
151.2 |
10056804 |
92.4% |
9292486 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,201,960 |
A→C |
D200E (GAT→GAG) |
iclR ← |
DNA‑binding transcriptional repressor |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,201,960 | 0 | A | C | 98.7%
| 653.9
/ ‑3.0
| 154 | D200E (GAT→GAG) | iclR | DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base C (83/69); total (83/71) |
| Fisher's exact test for biased strand distribution p-value = 2.11e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 |
Only 100 of 159 total aligned reads displayed.
CTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAG > REL606/4201887‑4202031
|
tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGctct < 1:3282471/76‑1 (MQ=255)
tGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtca < 2:2928309/76‑2 (MQ=255)
gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAGTAACCCCGTTTGCGCGTTTGGGCGAGCtctt < 2:3544363/76‑1 (MQ=255)
gCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtctt < 2:1433344/76‑1 (MQ=255)
cAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttc > 2:3493491/1‑76 (MQ=255)
aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttct < 1:1005882/76‑1 (MQ=255)
aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttct > 1:2470269/1‑76 (MQ=255)
aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtcttct > 1:484814/1‑76 (MQ=255)
aaGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCtctcct > 2:4999663/1‑76 (MQ=255)
aGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTcttctt < 2:2048356/76‑1 (MQ=255)
ggCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCttt < 2:1056942/76‑1 (MQ=255)
gCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCtttt < 2:723374/76‑1 (MQ=255)
cAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTa < 1:685044/76‑1 (MQ=255)
aaCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTaa < 2:4349314/76‑1 (MQ=255)
aaCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTaa < 1:3772948/76‑1 (MQ=255)
aCGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTaaa > 1:2231895/1‑76 (MQ=255)
cGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAAt > 1:1067545/1‑76 (MQ=255)
gTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATg > 2:624147/1‑76 (MQ=255)
tAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGc < 1:2683573/76‑1 (MQ=255)
aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:1863041/1‑76 (MQ=255)
aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:617953/1‑76 (MQ=255)
aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:493170/1‑76 (MQ=255)
aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 1:1278386/1‑76 (MQ=255)
aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 2:1913227/1‑76 (MQ=255)
aGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGca > 2:419286/1‑76 (MQ=255)
ccccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGcaca < 2:674283/76‑1 (MQ=255)
ccccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGcaca > 2:2749079/1‑76 (MQ=255)
cccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAg < 1:4172669/76‑1 (MQ=255)
ccAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAACTCTTCTTTTAAATGCACAgg < 1:4660783/76‑1 (MQ=255)
cAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGga > 2:1186693/1‑76 (MQ=255)
aGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGgag > 2:747054/1‑76 (MQ=255)
aGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGgag < 2:2319680/76‑1 (MQ=255)
aGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGgag > 1:1283386/1‑76 (MQ=255)
cgcATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGac < 2:4480668/76‑1 (MQ=255)
cgcATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGac > 2:2441711/1‑76 (MQ=255)
gcATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGaca < 2:1731454/76‑1 (MQ=255)
cATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGacac > 1:397175/1‑76 (MQ=255)
cATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGacac > 1:641653/1‑76 (MQ=255)
cATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGacac > 2:3362567/1‑76 (MQ=255)
tGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCa > 1:746656/1‑76 (MQ=255)
gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg > 1:4528958/1‑76 (MQ=255)
gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg < 2:2467550/76‑1 (MQ=255)
gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg > 1:1179115/1‑76 (MQ=255)
gTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAg > 1:1467354/1‑76 (MQ=255)
tCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCg > 1:1412189/1‑76 (MQ=255)
tCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCg > 2:568946/1‑76 (MQ=255)
cTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGtt < 2:2751335/76‑1 (MQ=255)
tCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTg < 2:2204200/76‑1 (MQ=255)
tCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTg < 1:3805966/76‑1 (MQ=255)
tCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGt > 1:3522426/1‑76 (MQ=255)
cGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTg < 2:822588/76‑1 (MQ=255)
gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGGGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 1:2349215/1‑76 (MQ=255)
gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 1:4050219/1‑76 (MQ=255)
gTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTgg > 2:2427313/1‑76 (MQ=255)
tCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTggg < 2:3877688/76‑1 (MQ=255)
tCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTggg > 2:247233/1‑76 (MQ=255)
cAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGt < 2:1230270/76‑1 (MQ=255)
cAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGt < 1:4166119/76‑1 (MQ=255)
aaaTGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGta > 1:2416908/1‑76 (MQ=255)
aaaTGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGta < 1:2299279/76‑1 (MQ=255)
aTGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGtata < 1:464615/76‑1 (MQ=255)
tGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAg > 2:3070142/1‑76 (MQ=255)
tGAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAg < 1:3856869/76‑1 (MQ=255)
gAATAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAgg > 2:1867983/1‑76 (MQ=255)
aaTAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc > 2:4839140/1‑76 (MQ=255)
aaTAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGc < 2:1885017/76‑1 (MQ=255)
aTAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCa > 1:4042782/1‑76 (MQ=255)
tAACCCCGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCAt > 1:210439/1‑76 (MQ=255)
cccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTaa > 2:4217907/1‑76 (MQ=255)
cccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTaa < 2:1722703/76‑1 (MQ=255)
cccGTTTGCGCGTTTGGACGAGCTCTTCGTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTaa < 2:4342284/76‑1 (MQ=255)
ccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc < 2:1528335/76‑1 (MQ=255)
ccGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAc < 1:4547315/76‑1 (MQ=255)
cGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc > 1:1668912/1‑76 (MQ=255)
cGTTTGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAAcc < 2:4656781/76‑1 (MQ=255)
tttGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCt < 1:2671411/76‑1 (MQ=255)
ttGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCtt > 2:4526916/1‑76 (MQ=255)
ttGCGCGTTTGGGCGAGCTCTTCTTTGAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCtt < 2:1300165/76‑1 (MQ=255)
tGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt < 2:300874/76‑1 (MQ=255)
tGCGCGTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCttt > 1:2179880/1‑76 (MQ=255)
cgcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGc > 1:4704828/1‑76 (MQ=255)
cgcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGCATAGGCATGGAACACTTTGc > 1:3765794/1‑76 (MQ=255)
gcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg > 1:1880842/1‑76 (MQ=255)
gcgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCg < 2:1937819/76‑1 (MQ=255)
cgTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCgg > 1:507295/1‑76 (MQ=255)
gTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt < 1:2994603/76‑1 (MQ=255)
gTTTGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGt > 1:3195924/1‑76 (MQ=255)
tttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTg < 2:3946642/76‑1 (MQ=255)
ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc < 1:3413187/76‑1 (MQ=255)
ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc < 2:4410526/76‑1 (MQ=255)
ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc > 1:2487719/1‑76 (MQ=255)
ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc > 2:1314482/1‑76 (MQ=255)
ttGGGCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgc > 1:1159122/1‑76 (MQ=255)
gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGTTgcag > 2:4982090/1‑76 (MQ=255)
gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 1:3602750/1‑76 (MQ=255)
gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 2:3616512/1‑76 (MQ=255)
gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 1:3386610/1‑76 (MQ=255)
gggCGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTgcag > 2:4181853/1‑76 (MQ=255)
cGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGcagcag > 2:3424684/1‑76 (MQ=255)
cGAGCTCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGcagcag > 1:3529525/1‑76 (MQ=255)
|
CTGCAAGGCAACGTAGCCCCAGCGCATGTTCCTCATCGTCAAATGAATAACCCCGTTTGCGCGTTTGGGCGAGATCTTCTTTTAAATGCACAGGAGACACCAGCGTTGCGTGGGTATAGGCATGTAACCCTTTGCGGTGCAGCAG > REL606/4201887‑4202031
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
