breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 649,391 T→G I471L (ATC→CTC)  mrdA ← transpeptidase involved in peptidoglycan synthesis (penicillin‑binding protein 2)
RA 743,241 A→G K310R (AAG→AGG)  sucB → dihydrolipoamide acetyltransferase
RA 931,164 T→G E134A (GAA→GCA)  ybjE ← predicted transporter
RA 1,014,635 (T)7→8 intergenic (‑32/‑324) ssuE ← / → ycbQ NAD(P)H‑dependent FMN reductase/predicted fimbrial‑like adhesin protein
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,609,130 A→C K285Q (AAG→CAG)  insF‑3 → putative transposase for IS3
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 1,822,155 (A)8→7 intergenic (‑56/+72) ynjI ← / ← topB predicted inner membrane protein/DNA topoisomerase III
RA 2,131,466 (A)9→8 intergenic (‑125/+175) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,002,366 G→T A935S (GCT→TCT)  flu → antigen 43 (Ag43) phase‑variable biofilm formation autotransporter
JC JC 3,014,531 IS150 (+) +3 bp coding (1112‑1114/1137 nt) ECB_02815 → Capsule polysaccharide export inner‑membrane protein kpsE
RA 3,136,084 A→G intergenic (+121/+42) cca → / ← uppP fused tRNA nucleotidyl transferase/2'3'‑cyclic phosphodiesterase/2'nucleotidase and phosphatase/undecaprenyl pyrophosphate phosphatase
JC 3,145,983 Δ9 bp coding (120‑128/1842 nt) rpoD → RNA polymerase sigma factor
RA 3,261,861 G→A F103F (TTC→TTT hflB ← protease, ATP‑dependent zinc‑metallo
RA 3,339,403 T→C M81T (ATG→ACG)  fis → DNA‑binding protein Fis
RA 3,464,820 C→T R150H (CGC→CAC)  ompR ← osmolarity response regulator
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
MC JC 3,843,787 Δ54 bp coding (251‑304/1404 nt) dnaA ← chromosomal replication initiation protein
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,911,991 (T)5→4 intergenic (‑192/‑131) yifB ← / → ilvL predicted bifunctional enzyme and transcriptional regulator/ilvG operon leader peptide
RA 4,163,752 T→G I966S (ATC→AGC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,179,127 C→A N4K (AAC→AAA yjaG → hypothetical protein
RA 4,201,960 A→C D200E (GAT→GAG iclR ← DNA‑binding transcriptional repressor
RA 4,396,897 A→T I295N (ATC→AAC)  yjfR ← predicted L‑ascorbate 6‑phosphate lactonase
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 761487–761513 761757–761646 134–271 55 [53] [51] 58 [lysT]–[lysW] [lysT],valT,[lysW]