breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 198 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | REL606 | 1,747,903 | 0 | G | T | 53.2% | 28.0 / inf | 363 | A8S (GCA→TCA) | ydiL | hypothetical protein |
* | REL606 | 2,515,909 | 0 | A | C | 48.9% | 25.5 / 34.3 | 45 | V260G (GTG→GGG) | ypfI | predicted hydrolase |
* | REL606 | 3,774,914 | 0 | C | G | 44.0% | 127.6 / 116.1 | 116 | A20P (GCC→CCC) | yicJ | predicted transporter |
* | REL606 | 571,979 | 0 | C | G | 42.9% | 13.4 / 10.6 | 21 | R20R (CGG→CGC) A504P (GCA→CCA) | ECB_00529 ECB_00530 | hypothetical protein conserved hypothetical protein |
* | REL606 | 2,437,406 | 0 | G | T | 41.2% | 20.0 / 10.2 | 17 | D435E (GAC→GAA) | ypdD | fused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component |
* | REL606 | 2,676,636 | 0 | T | G | 40.8% | 214.6 / 60.7 | 142 | V149G (GTG→GGG) | smpB | SsrA‑binding protein |
* | REL606 | 2,144,295 | 0 | A | C | 40.5% | 194.3 / 78.5 | 131 | G357G (GGT→GGG) | mrp | antiporter inner membrane protein |
* | REL606 | 1,854,401 | 0 | A | C | 40.0% | 77.6 / 23.5 | 60 | K88N (AAA→AAC) | yeaO | hypothetical protein |
* | REL606 | 433 | 0 | T | G | 39.7% | 80.7 / 20.3 | 58 | V33G (GTG→GGG) | thrA | bifunctional aspartokinase I/homeserine dehydrogenase I |
* | REL606 | 1,875,483 | 0 | G | C | 39.5% | 398.7 / inf | 375 | intergenic (+156/‑28) | yeaB/sdaA | predicted NUDIX hydrolase/L‑serine deaminase I |
* | REL606 | 239,163 | 0 | T | G | 37.7% | 164.0 / 74.4 | 114 | V58G (GTG→GGG) | dnaQ | DNA polymerase III subunit epsilon |
* | REL606 | 3,472,700 | 0 | C | G | 37.5% | 89.4 / 48.2 | 64 | A290G (GCG→GGG) | yhgA | predicted transposase |
* | REL606 | 4,570,990 | 0 | C | G | 37.5% | 48.7 / 20.6 | 40 | S250S (TCG→TCC) | hpaB | 4‑hydroxyphenylacetate 3‑monooxygenase |
* | REL606 | 720,464 | 0 | C | G | 36.4% | 23.8 / 14.3 | 22 | A22G (GCC→GGC) | phrB | deoxyribodipyrimidine photolyase, FAD‑binding |
* | REL606 | 1,906,430 | 0 | A | C | 36.1% | 119.2 / 33.4 | 72 | V329G (GTG→GGG) | ptrB | protease II |
* | REL606 | 3,378,885 | 0 | T | G | 35.2% | 239.1 / 31.4 | 125 | T129P (ACC→CCC) | rplB | 50S ribosomal protein L2 |
* | REL606 | 3,575,608 | 0 | T | G | 35.1% | 213.2 / 33.2 | 114 | T89P (ACC→CCC) | prlC | oligopeptidase A |
* | REL606 | 947,119 | 0 | A | G | 35.0% | 130.5 / 41.0 | 80 | F301S (TTC→TCC) | cydD | fused cysteine transporter subunits of ABC superfamily: membrane component/ATP‑binding component |
* | REL606 | 2,701,273 | 0 | C | G | 34.4% | 39.6 / 16.5 | 33 | A171G (GCG→GGG) | proW | glycine betaine transporter subunit |
* | REL606 | 4,320,225 | 0 | T | G | 34.3% | 152.2 / 54.0 | 99 | T40P (ACC→CCC) | melR | DNA‑binding transcriptional dual regulator |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | REL606 | 1951951 = | 140 (0.930) | 3 (0.020) | 3/132 | 12.7 | 2.3% | coding (159/504 nt) | cheW | purine‑binding chemotaxis protein |
? | REL606 | = 3717486 | 130 (0.990) | coding (4496/4851 nt) | ECB_03460 | putative adhesin | |||||
* | ? | REL606 | 4377157 = | 143 (0.950) | 3 (0.020) | 3/148 | 13.4 | 2.0% | coding (1591/1848 nt) | mutL | DNA mismatch repair protein |
? | REL606 | 4453855 = | 158 (1.070) | coding (336/387 nt) | yjgF | ketoacid‑binding protein |