breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 198 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*REL6061,747,9030GT53.2% 28.0 / inf 363A8S (GCA→TCA) ydiLhypothetical protein
*REL6062,515,9090AC48.9% 25.5 / 34.3 45V260G (GTG→GGG) ypfIpredicted hydrolase
*REL6063,774,9140CG44.0% 127.6 / 116.1 116A20P (GCC→CCC) yicJpredicted transporter
*REL606571,9790CG42.9% 13.4 / 10.6 21R20R (CGG→CGC
A504P (GCA→CCA) 
ECB_00529
ECB_00530
hypothetical protein
conserved hypothetical protein
*REL6062,437,4060GT41.2% 20.0 / 10.2 17D435E (GAC→GAAypdDfused predicted PTS enzymes: Hpr component/enzyme I component/enzyme IIA component
*REL6062,676,6360TG40.8% 214.6 / 60.7 142V149G (GTG→GGG) smpBSsrA‑binding protein
*REL6062,144,2950AC40.5% 194.3 / 78.5 131G357G (GGT→GGGmrpantiporter inner membrane protein
*REL6061,854,4010AC40.0% 77.6 / 23.5 60K88N (AAA→AACyeaOhypothetical protein
*REL6064330TG39.7% 80.7 / 20.3 58V33G (GTG→GGG) thrAbifunctional aspartokinase I/homeserine dehydrogenase I
*REL6061,875,4830GC39.5% 398.7 / inf 375intergenic (+156/‑28)yeaB/sdaApredicted NUDIX hydrolase/L‑serine deaminase I
*REL606239,1630TG37.7% 164.0 / 74.4 114V58G (GTG→GGG) dnaQDNA polymerase III subunit epsilon
*REL6063,472,7000CG37.5% 89.4 / 48.2 64A290G (GCG→GGG) yhgApredicted transposase
*REL6064,570,9900CG37.5% 48.7 / 20.6 40S250S (TCG→TCChpaB4‑hydroxyphenylacetate 3‑monooxygenase
*REL606720,4640CG36.4% 23.8 / 14.3 22A22G (GCC→GGC) phrBdeoxyribodipyrimidine photolyase, FAD‑binding
*REL6061,906,4300AC36.1% 119.2 / 33.4 72V329G (GTG→GGG) ptrBprotease II
*REL6063,378,8850TG35.2% 239.1 / 31.4 125T129P (ACC→CCC) rplB50S ribosomal protein L2
*REL6063,575,6080TG35.1% 213.2 / 33.2 114T89P (ACC→CCC) prlColigopeptidase A
*REL606947,1190AG35.0% 130.5 / 41.0 80F301S (TTC→TCC) cydDfused cysteine transporter subunits of ABC superfamily: membrane component/ATP‑binding component
*REL6062,701,2730CG34.4% 39.6 / 16.5 33A171G (GCG→GGG) proWglycine betaine transporter subunit
*REL6064,320,2250TG34.3% 152.2 / 54.0 99T40P (ACC→CCC) melRDNA‑binding transcriptional dual regulator

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 1951951 =140 (0.930)3 (0.020) 3/132 12.7 2.3% coding (159/504 nt) cheW purine‑binding chemotaxis protein
?REL606 = 3717486 130 (0.990)coding (4496/4851 nt) ECB_03460 putative adhesin
* ? REL606 4377157 =143 (0.950)3 (0.020) 3/148 13.4 2.0% coding (1591/1848 nt) mutL DNA mismatch repair protein
?REL606 4453855 = 158 (1.070)coding (336/387 nt) yjgF ketoacid‑binding protein