Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A122 F1 I1 R1
|
27 |
151.2 |
10056804 |
92.4% |
9292486 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
4,396,897 |
A→T |
I295N (ATC→AAC) |
yjfR ← |
predicted L‑ascorbate 6‑phosphate lactonase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 4,396,897 | 0 | A | T | 98.7%
| 586.6
/ 0.1
| 149 | I295N (ATC→AAC) | yjfR | predicted L‑ascorbate 6‑phosphate lactonase |
| Reads supporting (aligned to +/- strand): ref base A (0/2); new base T (54/93); total (54/95) |
| Fisher's exact test for biased strand distribution p-value = 5.35e-01 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.79e-01 |
Only 100 of 152 total aligned reads displayed.
CCGCCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAGG > REL606/4396825‑4396968
|
cgcccAACCTGCCAGATAAACGGCTCGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct < 2:2044977/73‑1 (MQ=255)
ccgccACCCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct < 1:1596178/76‑1 (MQ=255)
ccgccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct > 2:3053043/1‑76 (MQ=255)
cccccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtct < 2:51700/73‑1 (MQ=255)
cgccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTtctc > 1:4287505/1‑76 (MQ=255)
gccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTctct > 1:3760728/1‑76 (MQ=255)
ccAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCtt > 1:4948424/1‑76 (MQ=255)
cAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTg > 2:408509/1‑76 (MQ=255)
cAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTg < 2:2631408/76‑1 (MQ=255)
aCCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCg < 2:1922881/76‑1 (MQ=255)
aCCTGCCAGAAAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCg < 1:860816/76‑1 (MQ=255)
tGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGAt > 2:4495735/1‑76 (MQ=255)
gCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATc > 1:1228762/1‑76 (MQ=255)
ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 1:1400413/76‑1 (MQ=255)
ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 1:4212544/76‑1 (MQ=255)
ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 2:2614418/76‑1 (MQ=255)
ccAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCg < 1:3212366/76‑1 (MQ=255)
ccAGATAAACGGCTTGAAGCCATAATTCAGGAGATGGTTTTTCATTTCCCCCAGCACGCGGTTCTCTTGCGGGTCg > 2:1504141/1‑76 (MQ=255)
cAGAAAAAGGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCgg < 2:4731692/76‑1 (MQ=255)
aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGc > 1:2833423/1‑76 (MQ=255)
aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCCGc > 2:4329923/1‑76 (MQ=255)
aGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCCGc > 2:362975/1‑76 (MQ=255)
aTAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCt > 1:4367148/1‑76 (MQ=255)
tAAGCGGCTTAAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:4543423/76‑1 (MQ=255)
tAAACGGTTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:1644591/76‑1 (MQ=255)
tAAACGGCTGGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:3006006/76‑1 (MQ=255)
aaaaCGGCTTAAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTg < 2:255723/75‑1 (MQ=255)
aaaCGTTTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTgg < 2:1934828/76‑1 (MQ=255)
aaaCGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTgg > 1:1552219/1‑76 (MQ=255)
ccGGCTTAAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGaa < 2:4218559/75‑1 (MQ=255)
aCGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGaa < 1:2960588/76‑1 (MQ=255)
cGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAg < 1:3462426/76‑1 (MQ=255)
cGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAg < 1:1399829/76‑1 (MQ=255)
gCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGtt > 1:112981/1‑76 (MQ=255)
gCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGtt < 1:2971206/76‑1 (MQ=255)
ttGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTg < 2:4883984/76‑1 (MQ=255)
tGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGa < 2:3650325/76‑1 (MQ=255)
tGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGa > 1:1545845/1‑76 (MQ=255)
gAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGAc > 2:1577746/1‑76 (MQ=255)
aaGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGAcc < 1:1093281/76‑1 (MQ=255)
gCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAg < 2:1732071/76‑1 (MQ=255)
gCCATACTACAGGCGATCTTTTTTCATCTCCCACAGCTCGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAg < 2:5023093/76‑1 (MQ=255)
ccATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGa > 1:5011130/1‑76 (MQ=255)
cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat > 1:1838689/1‑76 (MQ=255)
cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat < 1:4620297/76‑1 (MQ=255)
cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat > 1:2609439/1‑76 (MQ=255)
cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat < 1:3438398/76‑1 (MQ=255)
cATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGat < 2:1218918/76‑1 (MQ=255)
aTACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata < 2:3836161/76‑1 (MQ=255)
aTACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata < 2:225078/76‑1 (MQ=255)
aTACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGata > 1:616807/1‑76 (MQ=255)
aCTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATc < 1:2206171/76‑1 (MQ=255)
tCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtg < 2:4126690/76‑1 (MQ=255)
cAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg < 1:877144/76‑1 (MQ=255)
cAGGCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCgtgg > 2:2393266/1‑76 (MQ=255)
gCGATCTTTTTTCGTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGtggtgg < 1:682995/76‑1 (MQ=255)
gCGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGtggtgg < 2:4156010/76‑1 (MQ=255)
cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa < 2:1503093/76‑1 (MQ=255)
cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa > 2:4951952/1‑76 (MQ=255)
cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGa < 1:1670898/76‑1 (MQ=255)
cGATCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGGGGa > 2:101707/1‑76 (MQ=255)
aTCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAAc > 1:4839639/1‑76 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 1:4279649/76‑1 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 2:4234361/76‑1 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 1:2053284/76‑1 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg > 2:3212193/1‑76 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 2:210912/76‑1 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 1:4238723/76‑1 (MQ=255)
tCTTTTTTCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACg < 2:1404413/76‑1 (MQ=255)
ttttttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACggg < 2:2696313/76‑1 (MQ=255)
ttttttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACggg < 1:2659841/76‑1 (MQ=255)
ttttttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGGGGAACggg > 2:3200160/1‑76 (MQ=255)
tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa < 1:2495106/76‑1 (MQ=255)
tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa < 1:2213216/76‑1 (MQ=255)
tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa < 1:4760071/76‑1 (MQ=255)
tttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATa > 1:4990749/1‑76 (MQ=255)
ttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATaa < 1:1822447/76‑1 (MQ=255)
ttCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATaa > 1:3664451/1‑76 (MQ=255)
tCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCCGATAACGTGGTGGAACGGGATAAc > 2:4382046/1‑76 (MQ=255)
tCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAc < 2:2075456/76‑1 (MQ=255)
tCATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAc > 1:3375320/1‑76 (MQ=255)
cATCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAcc < 1:4482219/76‑1 (MQ=255)
aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:3890328/76‑1 (MQ=255)
aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:2582498/76‑1 (MQ=255)
aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:4152902/76‑1 (MQ=255)
aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:114544/76‑1 (MQ=255)
aTCTCCCACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCa < 2:737896/76‑1 (MQ=255)
cccACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAAACACttt > 2:1338961/1‑76 (MQ=255)
ccACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTc < 1:3267604/76‑1 (MQ=255)
ccACAGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTc > 2:727825/1‑76 (MQ=255)
aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 2:2836372/76‑1 (MQ=255)
aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 2:1790292/76‑1 (MQ=255)
aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 1:2321595/76‑1 (MQ=255)
aGCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAt < 1:1258312/76‑1 (MQ=255)
gCACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCAtt < 2:921342/76‑1 (MQ=255)
cACGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTc > 2:4302549/1‑76 (MQ=255)
aCGCGGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCa > 1:2898818/1‑76 (MQ=255)
gcgGTTCTCTTGCGGATCGGCCTGTAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAgg < 2:302187/76‑1 (MQ=255)
gcgGTTCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAgg < 1:2116239/76‑1 (MQ=255)
gcgGTTCTCTCGCGGATCGGCCTGGAAATTTTAGCGGCTATCGTGGTGGGACGGGATAACCCCTTTAGGATTCacg > 2:2432612/1‑74 (MQ=255)
|
CCGCCAACCTGCCAGATAAACGGCTTGAAGCCATACTTCAGGCGATCTTTTTTCATCTCCCACAGCACGCGGATCTCTTGCGGATCGGCCTGGAAGTTTGACCAGATATCGTGGTGGAACGGGATAACCACTTTCGCATTCAGG > REL606/4396825‑4396968
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
