Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A43 F1 I1 R1
|
17 |
61.8 |
3886062 |
97.4% |
3785024 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,844,557 |
C→T |
R233W (CGG→TGG) |
argA → |
N‑acetylglutamate synthase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,844,557 | 0 | C | T | 100.0%
| 183.0
/ NA
| 48 | R233W (CGG→TGG) | argA | N‑acetylglutamate synthase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base T (28/20); total (28/20) |
TTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAG > REL606/2844484‑2844629
|
ttGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTgg < 1:42376/76‑1 (MQ=255)
tGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTggg > 2:740389/1‑76 (MQ=255)
ctTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGa > 2:991202/1‑76 (MQ=255)
tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAg > 2:1777798/1‑76 (MQ=255)
tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAg > 2:1202390/1‑76 (MQ=255)
tCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAg > 1:747986/1‑76 (MQ=255)
aGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCa > 2:1450038/1‑76 (MQ=255)
aGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCa > 2:1231667/1‑76 (MQ=255)
aGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCa < 1:930973/76‑1 (MQ=255)
gggCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAg < 2:991960/76‑1 (MQ=255)
gggCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAg < 2:704479/76‑1 (MQ=255)
gCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGa < 1:835615/76‑1 (MQ=255)
gTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAag < 1:1879314/76‑1 (MQ=255)
tCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCACGCGTGGGTAGAAGCCCAGGAaga > 1:1732436/1‑76 (MQ=255)
cACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAagag < 2:948524/76‑1 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga > 2:1840665/1‑76 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga > 2:282984/1‑76 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga < 1:946633/76‑1 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga > 1:832479/1‑76 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga < 1:821427/76‑1 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga < 1:299019/76‑1 (MQ=255)
aCTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAgaga < 1:1824588/76‑1 (MQ=255)
tAATGACGACTGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGaaa < 1:98617/76‑1 (MQ=255)
aaTGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAg > 2:1281548/1‑76 (MQ=255)
aaTGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAg > 1:1731646/1‑76 (MQ=255)
cgGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTan < 1:1916024/76‑2 (MQ=255)
gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACaa > 1:671210/1‑76 (MQ=255)
gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACaa > 2:510445/1‑76 (MQ=255)
gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACaa > 2:354810/1‑76 (MQ=255)
gTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTACAAGCCCAGGAAGAGAAAGGCGATTACaa > 1:1287996/1‑76 (MQ=255)
gATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACt < 1:1751358/76‑1 (MQ=255)
atatTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTc > 2:248137/1‑76 (MQ=255)
atatTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGCTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTc > 1:1152193/1‑76 (MQ=255)
tGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGt < 2:1908739/76‑1 (MQ=255)
gTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTa < 2:236994/76‑1 (MQ=255)
tctcAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTAc > 1:1206645/1‑76 (MQ=255)
tctcAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTAc < 2:1794693/76‑1 (MQ=255)
ctcAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACg < 1:150958/76‑1 (MQ=255)
cAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGt > 2:848539/1‑76 (MQ=255)
cAGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGt > 1:220157/1‑76 (MQ=255)
aGAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTg > 2:664937/1‑76 (MQ=255)
gAACTTTTCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTgc > 2:374928/1‑76 (MQ=255)
tttCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCttt < 2:1080491/76‑1 (MQ=255)
tttCCCTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCttt < 2:731130/76‑1 (MQ=255)
cccTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTg > 1:1556496/1‑76 (MQ=255)
ccTAACGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGc < 1:595438/76‑1 (MQ=255)
cGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGgc > 1:1472645/1‑76 (MQ=255)
cGAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGgc > 1:1711179/1‑76 (MQ=255)
gAAGCGCAAGCGTGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGgcg > 1:943998/1‑76 (MQ=255)
agcgcaagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCa > 1:1614943/1‑76 (MQ=255)
agcgcaagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCa > 1:1313731/1‑76 (MQ=255)
aagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGan < 1:373562/76‑2 (MQ=255)
aagcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGaa > 2:543968/1‑76 (MQ=255)
gcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAg < 1:492872/76‑1 (MQ=255)
gcgtGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAg < 1:1777798/76‑1 (MQ=255)
|
TTGCTCTTCCCAGGGCGTCACTAATGACGACGGTGATATTGTCTCAGAACTTTTCCCTAACGAAGCGCAAGCGCGGGTAGAAGCCCAGGAAGAGAAAGGCGATTACAACTCCGGTACGGTGCGCTTTTTGCGTGGCGCAGTGAAAG > REL606/2844484‑2844629
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
