breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC 547,700 Δ42,623 bp between IS1 [nmpC][insK‑1] 44 genes
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
RA 1,332,148 (T)6→5 intergenic (+131/‑79) topA → / → cysB DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding
MC JC 1,670,487 Δ9 bp coding (964‑972/1374 nt) uidB ← glucuronide transporter
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 2,781,131 T→C intergenic (+52/‑26) ygcM → / → ygcN 6‑pyruvoyl tetrahydrobiopterin synthase (PTPS)/predicted oxidoreductase with FAD/NAD(P)‑binding domain
RA 2,844,557 C→T R233W (CGG→TGG)  argA → N‑acetylglutamate synthase
RA 3,147,053 G→A R397H (CGT→CAT)  rpoD → RNA polymerase sigma factor
RA 3,339,247 A→G D29G (GAC→GGC)  fis → DNA‑binding protein Fis
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,929,372 A→G I386V (ATC→GTC)  rho → transcription termination factor Rho
RA 4,162,570 T→A I572N (ATC→AAC)  rpoB → DNA‑directed RNA polymerase subunit beta
RA 4,438,531 T→G D110A (GAC→GCC)  fbp ← fructose‑1,6‑bisphosphatase
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? REL606 3014207 =6 (0.100)53 (0.860) 42/150 0.4 89.8% coding (788/1137 nt) ECB_02815 Capsule polysaccharide export inner‑membrane protein kpsE
?REL606 = 3014214 NA (NA)coding (795/1137 nt) ECB_02815 Capsule polysaccharide export inner‑membrane protein kpsE