Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
20 |
54.5 |
3547384 |
95.1% |
3373562 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
2,031,529 |
G→A |
N423N (AAC→AAT) |
manB ← |
Phosphomannomutase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 2,031,529 | 0 | G | A | 100.0%
| 67.2
/ NA
| 24 | N423N (AAC→AAT) | manB | Phosphomannomutase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (10/14); total (10/14) |
TAAGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTGTTGGAGGAGCGCAGGTTAAAGCGCCAGTCGGCA > REL606/2031456‑2031562
|
tAAGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTAtt > 1:517569/1‑76 (MQ=255)
aGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTgg < 2:52339/76‑1 (MQ=255)
aGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTgg < 2:383533/76‑1 (MQ=255)
ttttttCTTTTCTCCCATCAGCGGTACATCCCCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTggag < 2:919307/76‑1 (MQ=17)
tGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGCATTGgaggag > 2:1056907/1‑76 (MQ=255)
tGTCTTTTCGTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTggaggtg < 1:1238376/76‑3 (MQ=14)
cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc > 1:439277/1‑76 (MQ=255)
cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc > 2:510068/1‑76 (MQ=255)
cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc > 2:455603/1‑76 (MQ=255)
cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc < 2:1105292/76‑1 (MQ=255)
cTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTgcgc > 2:231553/1‑76 (MQ=255)
tttCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAg > 2:1530243/1‑76 (MQ=255)
tttCTCCCATCAGCGGTACACCCCCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAg < 1:950435/76‑1 (MQ=14)
ttcttcCATCATCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg > 1:43378/1‑76 (MQ=11)
ttcttcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAgg > 1:655258/1‑76 (MQ=255)
cttcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt < 1:1570369/76‑1 (MQ=255)
cttcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt > 2:1584203/1‑76 (MQ=255)
cttcCATCAGCGGTACATCACCGCGCGATTCCACACTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGtt > 1:359395/1‑76 (MQ=11)
tcCATCAGCGGTACATCCCCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa < 1:1123992/76‑1 (MQ=11)
tcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa < 1:636921/76‑1 (MQ=255)
tcCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTaa < 2:845780/76‑1 (MQ=255)
aTCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCATNTTAAAgc < 2:1433434/76‑1 (MQ=11)
aTCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAgc < 1:348727/76‑1 (MQ=255)
tCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGCGCGCAGGTTAAAgcg < 1:156940/76‑1 (MQ=255)
cGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTATTGGAGGTGCGCAGGTTAAAGCGCCAg < 1:1567148/76‑1 (MQ=255)
aTCACCGCGCGATTCCACATTCCACCGGCCCGCCGGTACGGTGTTGTAGGAGCGCATGTTAATGCGTCAGTCGGCa < 1:1711268/76‑1 (MQ=17)
|
TAAGTTTTGTCTTTTCTTCCATCAGCGGTACATCACCGCGCGATTCCACATTCAACCGCACCACCGGTTCGGTGTTGGAGGAGCGCAGGTTAAAGCGCCAGTCGGCA > REL606/2031456‑2031562
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
