breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
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evidence | position | mutation | annotation | gene | description |
RA | 1,004,121 | C→T | intergenic (‑47/+555) | ompF ← / ← asnS | outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase |
JC JC | 1,124,928 | Δ2 bp :: IS186 (–) +8 bp | coding (1025‑1032/1536 nt) | mdoG → | glucan biosynthesis protein, periplasmic |
RA | 1,329,516 | C→T | H33Y (CAC→TAC) | topA → | DNA topoisomerase I |
JC | 1,332,137 | Δ27 bp | intergenic (+120/‑64) | topA → / → cysB | DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding |
JC | 1,733,297 | (CGTATGAA)1→2 | coding (333/1413 nt) | pykF → | pyruvate kinase |
RA | 2,031,529 | G→A | N423N (AAC→AAT) | manB ← | Phosphomannomutase |
RA | 2,031,537 | A→T | S421T (TCC→ACC) | manB ← | Phosphomannomutase |
JC | 2,103,918 | (CCAG)7→8 | coding (185/216 nt) | ECB_01992 → | hypothetical protein |
RA | 2,193,779 | G→T | L143I (CTT→ATT) | yeiB ← | conserved inner membrane protein |
RA | 3,145,846 | A→C | intergenic (+177/‑18) | dnaG → / → rpoD | DNA primase/RNA polymerase sigma factor |
RA | 3,165,608 | (A)8→7 | coding (1127/2019 nt) | fadH → | 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked |
RA | 3,264,050 | (A)7→6 | intergenic (‑101/‑147) | greA ← / → dacB | transcription elongation factor GreA/D‑alanyl‑D‑alanine carboxypeptidase |
RA | 3,367,986 | G→A | R317C (CGC→TGC) | rpoA ← | DNA‑directed RNA polymerase subunit alpha |
RA | 3,762,741 | A→T | K662I (AAA→ATA) | spoT → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
RA | 3,875,632 | (T)7→8 | intergenic (‑66/+287) | glmU ← / ← atpC | bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon |
RA | 3,893,551 | +G | intergenic (+6/‑50) | kup → / → insJ‑5 | potassium transporter/IS150 hypothetical protein |
MC JC | 3,894,997 | Δ6,934 bp | IS150‑mediated | rbsD–[yieO] | rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO] |
RA | 3,928,515 | A→C | K100T (AAG→ACG) | rho → | transcription termination factor Rho |
RA | 4,163,698 | T→G | I948S (ATC→AGC) | rpoB → | DNA‑directed RNA polymerase subunit beta |
JC JC | 4,524,522 | IS186 (+) +6 bp | coding (494‑499/549 nt) | fimA → | major type 1 subunit fimbrin (pilin) |
Unassigned missing coverage evidence | ||||||||||
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seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | REL606 | 2031542–2031570 | 2031767–2031729 | 160–226 | 20 [15] | [15] 17 | manB | Phosphomannomutase |
* | * | ÷ | REL606 | 2031858–2031887 | 2054816–2054779 | 22893–22959 | 17 [16] | [16] 17 | [manB]–[cpsG] | [manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,wzx,rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG] |