breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 1,004,121 C→T intergenic (‑47/+555) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl‑tRNA synthetase
JC JC 1,124,928 Δ2 bp :: IS186 (–) +8 bp coding (1025‑1032/1536 nt) mdoG → glucan biosynthesis protein, periplasmic
RA 1,329,516 C→T H33Y (CAC→TAC)  topA → DNA topoisomerase I
JC 1,332,137 Δ27 bp intergenic (+120/‑64) topA → / → cysB DNA topoisomerase I/DNA‑binding transcriptional dual regulator, O‑acetyl‑L‑serine‑binding
JC 1,733,297 (CGTATGAA)1→2 coding (333/1413 nt) pykF → pyruvate kinase
RA 2,031,529 G→A N423N (AAC→AAT manB ← Phosphomannomutase
RA 2,031,537 A→T S421T (TCC→ACC)  manB ← Phosphomannomutase
JC 2,103,918 (CCAG)7→8 coding (185/216 nt) ECB_01992 → hypothetical protein
RA 2,193,779 G→T L143I (CTT→ATT)  yeiB ← conserved inner membrane protein
RA 3,145,846 A→C intergenic (+177/‑18) dnaG → / → rpoD DNA primase/RNA polymerase sigma factor
RA 3,165,608 (A)8→7 coding (1127/2019 nt) fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
RA 3,264,050 (A)7→6 intergenic (‑101/‑147) greA ← / → dacB transcription elongation factor GreA/D‑alanyl‑D‑alanine carboxypeptidase
RA 3,367,986 G→A R317C (CGC→TGC)  rpoA ← DNA‑directed RNA polymerase subunit alpha
RA 3,762,741 A→T K662I (AAA→ATA)  spoT → bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase
RA 3,875,632 (T)7→8 intergenic (‑66/+287) glmU ← / ← atpC bifunctional N‑acetylglucosamine‑1‑phosphate uridyltransferase/glucosamine‑1‑phosphate acetyltransferase/F0F1 ATP synthase subunit epsilon
RA 3,893,551 +G intergenic (+6/‑50) kup → / → insJ‑5 potassium transporter/IS150 hypothetical protein
MC JC 3,894,997 Δ6,934 bp IS150‑mediated rbsD[yieO] rbsD, rbsA, rbsC, rbsB, rbsK, rbsR, [yieO]
RA 3,928,515 A→C K100T (AAG→ACG)  rho → transcription termination factor Rho
RA 4,163,698 T→G I948S (ATC→AGC)  rpoB → DNA‑directed RNA polymerase subunit beta
JC JC 4,524,522 IS186 (+) +6 bp coding (494‑499/549 nt) fimA → major type 1 subunit fimbrin (pilin)

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ REL606 2031542–2031570 2031767–2031729 160–226 20 [15] [15] 17 manB Phosphomannomutase
* * ÷ REL606 2031858–2031887 2054816–2054779 22893–22959 17 [16] [16] 17 [manB]–[cpsG] [manB],manC,insB‑14,insA‑14,wbbD,wbbC,wzy,wbbB,wbbA,vioB,vioA,wzx,rmlC,rfbA,rfbD,rfbB,galF,wcaM,wcaL,wcaK,wzxC,wcaJ,[cpsG]