Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A7 F1 I1 R1
|
20 |
54.5 |
3547384 |
95.1% |
3373562 |
76.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,515 |
A→C |
K100T (AAG→ACG) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,515 | 0 | A | C | 100.0%
| 91.0
/ NA
| 25 | K100T (AAG→ACG) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (16/9); total (16/9) |
CTGATGACATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAC > REL606/3928443‑3928586
|
cTGATGACATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGAt > 2:1029067/1‑76 (MQ=255)
tgatgaCATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGAtt < 1:1189095/76‑1 (MQ=255)
atgaCATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTcg > 1:529560/1‑76 (MQ=255)
gaCATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTcgcc < 2:782011/76‑1 (MQ=255)
cATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTcgcccg < 1:126962/76‑1 (MQ=255)
tCTACGTTTCCCTTAGCCANANCCGCCGTNTCAACCTCCGCACTGGTGATANCANCTCTGGTACGATTcgcccgcc < 2:257017/76‑1 (MQ=255)
cTACGTTCCCCGTACCCAACCCCGCCTTTTCACCCTCCGCCCTGGTGATACCATCTCTGGTACGATTCGCccgccg < 2:720782/76‑1 (MQ=255)
tACTTTCCCCTTACCCAAACCCGCCGTTTCACCCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGa < 2:1145361/76‑1 (MQ=255)
cGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGaaa > 1:525166/1‑76 (MQ=255)
tttCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAaaga > 2:819257/1‑76 (MQ=255)
ttccccTTACCCAAACCCGCCGTTTCACCCCCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAaaga < 2:1182059/73‑1 (MQ=255)
tCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAaagaag > 1:1362799/1‑76 (MQ=255)
ccccTTCCCCAACCCCGCCTTTTCACCCCCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAaagaag < 1:1258767/75‑1 (MQ=255)
cTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACNATCTCTGGTACGATTCGCNCGNCGAAAGAAGGTg > 1:1440037/1‑76 (MQ=255)
cTTACCCAACCCCGCCGTTCCACCCCCCGCGCTGGTAATCCCATCTCGGGTAGGATTCGCCCGCCGAAAGAAGGTg < 2:877756/76‑1 (MQ=255)
aGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAAc > 1:1679040/1‑76 (MQ=255)
aTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTAtt > 2:1087273/1‑76 (MQ=255)
tCACCCCCCGCACGGGTAATCCCATCTCTGGTACGTTTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCgctgct < 2:84647/76‑1 (MQ=255)
cacccTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGctgctg < 2:1494404/73‑1 (MQ=255)
cAACCTCCGCACCGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGctgctg > 2:462676/1‑76 (MQ=255)
cccccGCACTGGTAATACCATCCCGGTTACGATCCCCCCGCCGAAAGAAGGTGAACGCTATTGTGCGCTGCTGaaa < 1:955007/73‑1 (MQ=255)
ccGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt > 2:195132/1‑76 (MQ=255)
cGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa > 1:1381264/1‑76 (MQ=255)
cGCACGGGTGATCCCCTCTCTGGTACGATCCCCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa < 1:1029080/76‑1 (MQ=255)
cTGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa > 2:807037/1‑76 (MQ=255)
tGGTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGaa < 2:1647086/76‑1 (MQ=255)
ggTGATACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg > 2:969880/1‑76 (MQ=255)
tGATCCCATCTCTGGTACGTTCCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt < 1:884534/76‑1 (MQ=255)
tGATCCCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt < 2:148767/76‑1 (MQ=255)
tACCATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAAc > 1:629938/1‑76 (MQ=255)
ccctctcGGGTACGTTCCCCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt < 1:942042/73‑1 (MQ=255)
ccATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt < 1:1253065/76‑1 (MQ=255)
ccATCTCGGGTGCGAGTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCAGCTGAAAGTNAACGAAGTTAACtt < 1:613/76‑1 (MQ=255)
cATCTCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc > 2:314178/1‑76 (MQ=255)
cATCTCTGGTACGATCCCCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc < 2:834073/76‑1 (MQ=255)
aTCGCTGGTACGATTCGCCCGCCGAAAGAAGGTGAACGCTAGGTTGCGCTGCTGNAAGTTAACTANGTTTACTTCg < 2:1734430/76‑1 (MQ=255)
tctcGGGTACGATCCCCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGa < 2:757731/76‑1 (MQ=255)
tctGGTGCGATCCCCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACa < 2:1226040/76‑1 (MQ=255)
tgggTAAGATCCCCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa < 1:1373427/74‑1 (MQ=255)
ggTACGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAc > 2:1053695/1‑76 (MQ=255)
|
CTGATGACATCTACGTTTCCCTTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAC > REL606/3928443‑3928586
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
