Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A85 F1 I1 R1
|
15 |
51.9 |
3515760 |
95.5% |
3357550 |
75.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
REL606 |
3,928,593 |
A→T |
N126I (AAC→ATC) |
rho → |
transcription termination factor Rho |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | REL606 | 3,928,593 | 0 | A | T | 93.6%
| 160.4
/ 0.1
| 47 | N126I (AAC→ATC) | rho | transcription termination factor Rho |
| Reads supporting (aligned to +/- strand): ref base A (2/1); new base T (24/20); total (26/21) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGG > REL606/3928521‑3928665
|
gcccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGc > 1:139173/1‑76 (MQ=255)
cccgccGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGcc > 1:587338/1‑76 (MQ=255)
gccgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGc < 1:657926/76‑1 (MQ=255)
cgAAAGAAGGTAAACGCTATTTTGCGCTGCTGAAAGGTAACGAAGTTAACTTCGACAAACAAAAAATCGCCCGcaa < 2:1366485/76‑1 (MQ=255)
gAAAGAAGGTGAACGTTATTTTGCGCTGCTAAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGcaac < 1:1400705/76‑1 (MQ=255)
aagGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAAt < 1:234555/76‑1 (MQ=255)
agGTGAACGCTATTTTGCGCTGCTGAAAGTTAACAAATTAACCTTCGACAAACCTGAAATCGCCCGCAACAAAATc < 2:932633/76‑1 (MQ=255)
aCGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCttt < 2:1316398/76‑1 (MQ=255)
cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTg < 1:1366599/76‑1 (MQ=255)
cGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTg > 1:1556822/1‑76 (MQ=255)
cTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTgag > 1:262021/1‑76 (MQ=255)
cTATTTTGCGCTGCTAAAAGTTAACGAATTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTgag < 1:380663/76‑1 (MQ=255)
tATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTgaga > 1:1232215/1‑76 (MQ=255)
aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGaa > 2:344805/1‑76 (MQ=255)
aTTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGaa > 2:517059/1‑76 (MQ=255)
aTTTTGCGCTGATGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGaa > 1:1307249/1‑76 (MQ=255)
aTTTTGAGCTGCTGAAAGTTAACGAGGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGaa < 1:103603/76‑1 (MQ=255)
ttttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAAc > 2:985344/1‑76 (MQ=255)
ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACtt < 2:1020826/76‑1 (MQ=255)
gcgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTaa < 1:432133/76‑1 (MQ=255)
cgcTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAc > 1:524800/1‑76 (MQ=255)
cgcTGCTGAAAGTAAACGAATTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAc < 2:759948/76‑1 (MQ=255)
gctgctGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc > 1:1688926/1‑76 (MQ=255)
gctgctCAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc > 2:908686/1‑76 (MQ=255)
ctgctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc > 1:803132/1‑76 (MQ=255)
tgctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAAcccc < 2:1714402/76‑1 (MQ=255)
gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 1:909576/1‑76 (MQ=255)
gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 1:909595/1‑76 (MQ=255)
gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 1:131582/1‑76 (MQ=255)
gctgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg > 2:1624107/1‑76 (MQ=255)
tgAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt > 2:422826/1‑76 (MQ=255)
gAAAGTTAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg > 2:202719/1‑76 (MQ=255)
tAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 2:971673/1‑76 (MQ=255)
tAACGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa > 1:1745897/1‑76 (MQ=255)
cGAAGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc > 1:1558641/1‑76 (MQ=255)
aaGTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc < 2:275650/76‑1 (MQ=255)
aaGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc > 2:430257/1‑76 (MQ=255)
gTTAACTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc < 1:76160/76‑1 (MQ=255)
gTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACtctc > 2:1702843/1‑76 (MQ=255)
cTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg < 1:1310433/76‑1 (MQ=255)
cTTCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg < 2:1053783/76‑1 (MQ=255)
ttCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc < 2:1073674/76‑1 (MQ=255)
ttCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc > 2:1052025/1‑76 (MQ=255)
tCGACAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCg < 1:440310/76‑1 (MQ=255)
cAAACCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg < 1:1654576/76‑1 (MQ=255)
aCCTGAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa < 1:908686/76‑1 (MQ=255)
gAAATCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg > 2:572234/1‑76 (MQ=255)
aaatCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg > 2:59527/1‑76 (MQ=255)
|
GCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGG > REL606/3928521‑3928665
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
